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Rapid and Simple Method for the Determination in Sialic Acid of Yak Milk Fat Globule Membrane
Dehai Li 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.5
A rapid and simple method for the determination of sialic acid (Neu5Ac) of yak milk fat globule membrane (MFGM) by HPLC with a diode array detector was developed and validated. Samples were cleaned up just by hydrolysis and derivatization before HPLC analysis. Separation was achieved with an Agilent TC-C18 column. The method showed a good linearity (r=0.997), the sensitivity results showed that the limits of detection and limits of quantification for sialic acid were 10.0 and 21.0 μg/mL,respectively. The recovery of Neu5Ac was 95-97%. The method proved very simple and rapid for Neu5Ac analysis since separation was completely achieved at 12 min.
A New Cytotoxic Phenazine Derivative from a Deep Sea Bacterium Bacillus sp.
Dehai Li,Fengping Wang,Xiang Xiao,Xiang Zeng,Qian-Qun Gu,Weiming Zhu 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.5
A novel phenazine derivative (1) together with six known compounds (2-7) were isolated by bioassay-guided fractionation from the culture broth of a bacterium, Bacillus sp., collected from a Pacific deep sea sediment sample (depth 5059 m). The structures of these compounds were determined using spectroscopic methods. Their cytotoxic effects on P388 and K562 cell lines were preliminarily examined using the sulforhodamine-B (SRB) assay.
Cell Division Cycle Associated 8 Is a Key Regulator of Tamoxifen Resistance in Breast Cancer
Dehai Yu,Libo Shi,Yuhui Bu,Weidong Li 한국유방암학회 2019 Journal of breast cancer Vol.22 No.2
Purpose: Breast cancer (BC) is one of the most common malignancies globally, and millions of women worldwide are diagnosed with BC every year. Up to 70% of BC patients are estrogen receptor (ER)-positive. Numerous studies have shown that tamoxifen has a significant therapeutic effect on both primary and metastatic ER-positive BC patients. Although tamoxifen is currently one of the most successful therapeutic agents for BC, a significant proportion of patients will eventually become resistant to tamoxifen, leading to tumor recurrence and metastasis. Knowledge about the development of tamoxifen resistance in BC patients is still limited. Methods: We applied a loss-and-gain method to study the biological functional role of cell division cycle associated 8 (CDCA8) in tamoxifen resistance in BC cells. Results: We found that CDCA8 was significantly elevated in tamoxifen-resistant BC cells. Knockdown of CDCA8 expression significantly inhibited the proliferation of tamoxifen-resistant BC cells and reduced their resistance to tamoxifen. In contrast, overexpression of CDCA8 promoted the growth of tamoxifen-sensitive BC cells and induced their resistance to tamoxifen. Conclusion: In this study, we reported that CDCA8 is a key regulator of tamoxifen resistance in BC, suggesting that CDCA8 may serve as a potential therapeutic target for BC treatment.
A New Cytotoxic Phenazine Derivative from a Deep Sea Bacterium Bacillus sp
Zhu, Weiming,Li, Dehai,Wang, Fengping,Xiao, Xiang,Zeng, Xiang,Gu, Qian-Qun 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.5
A novel phenazine derivative (1) together with six known compounds (2-7) were isolated by bioassay-guided fractionation from the culture broth of a bacterium, Bacillus sp., collected from a Pacific deep sea sediment sample (depth 5059 m). The structures of these compounds were determined using spectroscopic methods. Their cytotoxic effects on P388 and K562 cell lines were preliminarily examined using the sulforhodamine-B (SRB) assay.
Two Indolocarbazole Alkaloids with Apoptosis Activity from a Marine-derived Actinomycete Z2039-2
Rui Liu,Tianjiao Zhu,Dehai Li,Jingyan Gu,Wei Xia,Yuchun Fang,HongbingLiu,Weiming Zhu,Qianqun Gu 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.3
Bioassay-guided fractionation of the EtOAc extract from the fermentation broth of a marinederived actinomycete Z2039-2 led to the isolation of two known indolocarbazole alkaloids, K252c (1) and arcyriaflavin A (2). 1 and 2 exhibited moderate cytotoxic activities against the K562 cell line, and induced apoptotic activities at 10 and 100 µM, respectively. This is the first report on the significant apoptosis inducing effect of indolocarbazole alkaloids against K562 cancer cells.
Meng Kang,Chenglong Li,Dehai Yu,Guodong Li,Zhaoping Song,Huili Wang,Wenxia Liu 한국섬유공학회 2022 Fibers and polymers Vol.23 No.7
Cellulose nanopaper has attracted tremendous attention as an alternative to fossil-based flexible substrates. However, the fabrication of nanopaper from cellulose nanofibers (CNFs) is a time-consuming process. Herein, positivelycharged CNFs (PCNFs) were employed to heterocoagulate negatively charged CNFs (NCNFs) at various PCNF-to-NCNFratios in order to decrease the formation time of nanopaper. At a PCNF-to-NCNF ratio of 1:1, the formation time was reducedfrom 98 min to 20 min. Although heterocoagulation increases the roughness of cellulose nanopaper, nanopapers made from amixture of NCNFs and PCNFs still possess a nanosized network structure and therefore show similar transparency tonanopapers made of pure NCNFs. Compared to the nanopaper made from pure NCNFs, the nanopaper made from themixture of NCNFs and PCNFs showed reduced tensile strength but improved resistance to deformation. This study providesa practical method for the production of cellulose nanopaper.
Two Indolocarbazole Alkaloids with Apoptosis Activity from a Marine-derived Actinomycete Z$_2$039-2
Liu, Rui,Zhu, Tianjiao,Li, Dehai,Gu, Jingyan,Xia, Wei,Fang, Yuchun,Zhu, Weiming 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.3
Bioassay-guided fractionation of the EtOAc extract from the fermentation broth of a marine-derived actinomycete Z$_2$039-2 led to the isolation of two known indolocarbazole alkaloids, K252c (1) and arcyriaflavin A (2). 1 and 2 exhibited moderate cytotoxic activities against the K562 cell line, and induced apoptotic activities at 10 and 100 ${\mu}$M, respectively. This is the first report on the significant apoptosis inducing effect of indolocarbazole alkaloids against K562cancer cells.
Furong Xin,Huili Wang,Feixiang Guan,Guodong Li,Zhaoping Song,Dehai Yu,Wenxia Liu 한국섬유공학회 2020 Fibers and polymers Vol.21 No.9
Paper-based material is receiving more and more attention as an alternative of plastics in flexible electronics. However, conventional paper made of cellulose fibers is opaque owing to its micron-sized void space among fibers. Herein,cellulose fiber paper was changed into transparent paper by sequentially coating cationic cellulose nanofibers (CNFs) andpolyvinylpyrrolidone (PVP). The morphology, transparency, thermal and mechanical properties were analyzed. The resultsshow that the coating of CNFs reduces the micron-sized void space in the cellulose fiber paper, favoring the furtherimprovement on the transparency of paper by coating PVP. By optimizing the coating amount of CNFs and PVP, atransparent paper with a transmittance of 88.5 % at 550 nm is obtained. The as-prepared transparent paper also showsimproved thermal stability, slightly increased tensile strength and significantly enhanced deformation resistance. It was apotential candidate of flexible electronic substrates.