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Dwi Sendi Priyono,Dedy Duryadi Solihin,Achmad Farajallah,Bambang Purwantara 국립중앙과학관 2020 Journal of Asia-Pacific Biodiversity Vol.13 No.2
The mitochondrial genome is able to provide important information for evolutionary biology. Therefore,this research aims at characterizing that of Bubalus quarlesi mitochondrial genome. Furthermore, it wasentirely 16,354 bp in length, consisting of 13 protein coding genes (PCGs), 22 tRNA, and two rRNA genes,one putative control region. All PCGs, however, used a typical ATN as start codon, while the stop codonwere typically TAA, TGA, and TGA, with the exception of nad3 and nad6 genes using TCA. It was alsoobserved that the lowest evolution rate of PCG was cox1, while the highest was atp8. Meanwhile, aphylogenetic analysis based on 13 PCGs was performed on 14 bovine species, using Bayesian andmaximum likelihood, and its tree indicated that B. quarlesi belongs to the subtribe of Bubalina, closelyrelated to Bubalus depressicornis. These, therefore, provide novel meaningful insights for the conservationgenetics and population genetics of B. quarlesi.
Endah Retno Palupi,Sudarsono Sudarsono,Sjamsoe’oed Sadjad,Dedy Duryadi Solihin,John N. Owens 한국산림과학회 2023 Forest Science And Technology Vol.19 No.3
Teak is an insect-pollinated species, and seed production depends on pollinators. The objec- tives of this study were to determine potential pollinators of teak and their foraging behav- ior in obtaining alternate food sources as the basis for management recommendations. Four traps, i.e. Moczarsky-Winkler selector traps, sticky traps, yellow-pan traps, and manual traps (insect net), were put up among blooming inflorescences during the flowering period of March to May. Insects were collected and counted weekly and identified. The presence and location of the pollen on their bodies were observed. The time and duration of foraging behavior, the duration of a single visit, the number of visits in an hour, and the landing pos- ition when approaching the teak flower as well as the understory were also observed. The result showed Ceratina sp., and Braunsapis sp. (Apidae), Nomia sp. (Halictidae) were potential teak pollinators in the CSO in East Java. The insect foraging behavior supports the ability to deposit pollen onto the stigma. The presence of Mimosa pudica in the understory plays as an alternate food source for pollinators. Intensive weeding and trimming of old or dead branches should be less practiced to enhance pollinators’ populations and nesting sites.
Zulfi Nur Amrina Rosyada,Berlin Pandapotan Pardede,Ekayanti Mulyawati Kaiin,Ligaya I.T.A Tumbelaka,Dedy Duryadi Solihin,Bambang Purwantara,Mokhamad Fakhrul Ulum Asian Australasian Association of Animal Productio 2023 Animal Bioscience Vol.36 No.12
Objective: This study aims to identify heat shock protein70-2 (HSP70-2) and protamine-1 (PRM1) mRNA and protein in Madura bull sperm and demonstrate their relation as bull fertility biomarkers. Methods: The Madura bull fertility rates were grouped based on the percentage of first service conception rate (%FSCR) as high fertility (HF) (79.04%; n = 4), and low fertility (LF) (65.84%; n = 4). mRNA of HSP70-2 and PRM1 with peptidylprolyl isomerase A (PPIA) as a housekeeping gene were determined by quantitative real-time polymerase chain reaction, while enzyme-linked immunoassay was used to measure protein abundance. In the post-thawed semen samples, sperm motility, viability, acrosome integrity, and sperm DNA fragmentation index were analyzed. Data analysis was performed on the measured parameters of semen quality, relative mRNA expression, and protein abundance of HSP70-2 and PRM1, among the bulls with various fertility levels (HF and LF) in a one-way analysis of variance analysis. The Pearson correlation was used to analyze the relationship between semen quality, mRNA, proteins, and fertility rate. Results: Relative mRNA expression and protein abundance of HSP70-2 and PRM1 were detected and were found to be highly expressed in bulls with HF (p<0.05) and were associated with several parameters of semen quality. Conclusion: HSP70-2 and PRM1 mRNA and protein molecules have great potential to serve as molecular markers for determining bull fertility.