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María de la Luz Galván-Ramírez,Ana Luisa Madriz-Elisondo,Jorge de Jesús Romero Rameño,Dania Araceli de la O Carrasco,Marco Antonio Cardona López,Cynthia Guadalupe Temores Ramírez 질병관리본부 2019 Osong Public Health and Research Persptectives Vol.10 No.1
Objectives: To determine the prevalence and risk factors associated with intestinal parasites in the population of San Juan Cosala, Jalisco, Mexico. Methods: A total of 277 samples from 104 participants were analysed using direct smear, flotation, formaldehyde/ethyl acetate, and modified Kinyoun’s acid-fast stain methods. The Graham method was applied only for samples from children under 12 years of age for the diagnosis of Enterobius vermicularis. Results: The prevalence of parasite infections in the study population was 77.9% including: Entamoeba histolytica/E. dispar/E. moshkovskii/E. bangladeshi (37.5%), Giardia intestinalis (11.5%); commensals: Endolimax nana (44.2%), Entamoeba coli (27.9%), Chilomastix mesnili (6.7%) and Iodamoeba bütschlii, (2.9%); emerging intestinal protozoans: Blastocystis spp. (49%), Cryptosporidium spp. (7.7%) and Cyclospora cayetanensis (2.9%); and helminths: Enterobius vermicularis (18.3%) and Ascaris lumbricoides (5.8%). The results also showed that 58.64% of the studied population presented polyparasitism. A significant association was found between protozoan infections and housewives, and houses that were not built with concrete ceilings, brick walls and cement floors (p < 0.05). Conclusion: Polyparasitism was observed in over half the study population. The most prevalent parasite was Blastocystis spp, whilst the prevalence of helminths was less than that of protozoans. The risk factors for infection to intestinal parasites were being a housewife and not having solid brick, cement and concrete materials for house construction.
A Girl with 15q Overgrowth Syndrome and dup(15)(q24q26.3) that Included Telomeric Sequences
María de los Ángeles Gutiérrez-Franco,María de la Luz Madariaga-Campos,Ana I. Vásquez-Velásquez,Esmeralda Matute,Roberto Guevara-Yáñez,Horacio Rivera 대한진단검사의학회 2010 Annals of Laboratory Medicine Vol.30 No.3
Distal 15q trisomy or tetrasomy is associated with a characteristic phenotype that includes mild to moderate intellectual disability, abnormal behavior, speech impairment, overgrowth, hyperlaxity, long face, prominent nose, puffy cheeks, pointed chin, small ears, and hand anomalies (mainly arachno- and camptodactyly). We present the case of a 13-yr-old girl with the main clinical features of 15q overgrowth syndrome and a 46,XX,dup(15)(q24q26.3)[117]/46,XX[3].ish dup(15)(q24q26.3) (SNPRN+,PML+,subtel++,tel++) de novo karyotype. The findings in this case are consistent with those in the previous distal 15q trisomy cases that presented with overgrowth and mental retardation. Further, the rearranged chromosome had a double set of directly oriented telomeric and subtelomeric sequences. (Korean J Lab Med 2010;30:318-24)
Victor E. Balderas Hernandez,Luz M. T. Paz Maldonado,Emilio Medina Rivero,Ana P. Barba de la Rosa,Leandro G. Ordonez Acevedo,Antonio De Leon Rodriguez 한국생물공학회 2008 Biotechnology and Bioprocess Engineering Vol.13 No.1
The production of human interferon gamma (hIFN-γ) using a synthetic gene in Escherichia coli BL21-SI was optimized by response surface methodology (RSM) and a Box-Behnken design. The process variables studied were temperature, biomass concentration at induction time and the NaCl concentration as inducer. According to the Box-Behnken design, a second order response function was developed. The optimal expression conditions were a temperature of 32.6℃, induction biomass of 0.31 g/L and 0.3 M NaCl in minimal medium. The model prediction for the maximum hIFN-γ production was 77.3 mg/L, which corresponded satisfactorily with the experimental data. The hIFN-γ concentration attained under optimized conditions was 13-times higher than that obtained using the non-optimized conditions. We conclude that RSM is an effective method for the optimization of recombinant protein expression using synthetic genes in E. coli.
Could Acupuncture Be Useful in the Treatment of Temporomandibular Dysfunction?
Ca´ssia Maria Grillo,Giancarlo De la Torre Canales,Ronaldo Seichi Wada,Marcelo Correˆa Alves,Ce´lia Marisa Rizzatti Barbosa,Fausto Berzin,Maria da Luz Rosa´rio de Sousa 사단법인약침학회 2015 Journal of Acupuncture & Meridian Studies Vol.8 No.4
In this study, the effects of acupuncture in comparison with flat occlusal plane appliance were evaluated in patient with myogenic temporomandibular dysfunction (TMD). The sample consisted of 40 women with TMD and unbalanced energy predominance of Yang Liver Ascension, selected using the Renying and Cunkou pulses, randomly divided into two groups: acupuncture and splint. The effect of treatments on the masseter and anterior temporal muscles was evaluated after 4 weeks of treatment, by means of electromyographic activity (root mean square) and pain pressure threshold. Pain intensity was measured using the visual analog scale, and range of mouth opening was evaluated using a millimeter ruler. All evaluations were performed at the beginning and end of the treatment. Visual analog scale score was reduced equally in the two groups (p < 0001), and the increase in range of mouth opening was significant in both groups. A significant difference was detected only in pain pressure threshold of the left masseter in the acupuncture group (p < 0.05). Only root mean square in the at rest position of the right temporal muscle diminished in the final stage of the splint group (p < 0.05). Both treatments reduced the pain intensity of myogenic TMD in the short term and may be considered strategies for control of chronic pain related to TMD.
E. E. Pérez‑Ramírez,M. de la Luz‑Asunción,A. L. Martínez‑Hernández,G. de la Rosa‑Álvarez,S. Fernández‑Tavizón,P. Salas,C. Velasco‑Santos 한국탄소학회 2019 Carbon Letters Vol.29 No.2
One- and two-dimensional carbon nanomaterials were tested as adsorbents for the elimination of two anionic dyes, reactive red 2 and methyl orange, and the cationic dye methylene blue from aqueous solutions under the same conditions. Carbon nanomaterials performed well in the removal of dyes. Surface oxygenated groups in the nanomaterials improved the cationic dyes’ adsorption, but not the adsorption of the anionic dye. The interactions between nanomaterials and dyes were verified by infrared and Raman spectroscopy. The pseudo-second order kinetic model was better fitted to the kinetic experimental data than the Elovich and pseudo-first order models. The equilibrium adsorption data were best fitted by the Langmuir model. The dimensions and morphology of the carbon nanomaterials play an important role in the adsorption of the three dyes. The main mechanism of adsorption of anionic dyes is by the interactions of the aromatic rings of the dye structures and π delocalized electrons on carbon nanostructures; the adsorption of cationic dye is mainly due to electrostatic interactions.
Extraction of Pectinesterase from Jalapeno Chili Pepper (Capsicum annuum) and Its Thermal Stability
Sonia Marisela Mejia-Cordova,Julio Cesar Montanez,Cristobal Noe Aguilar,Maria de la Luz Reyes-Vega,Heliodoro de la Garza,Roque Alberto Hours,Juan Carlos Contreras-Esquivel 한국식품과학회 2005 Food Science and Biotechnology Vol.14 No.2