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- 검색키워드
- 저자 : De Sotto, R. (검색결과 9 건)
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De Sotto, R. B.,Kim, K. I.,Kim, S.,Song, K. G.,Park, Y. IWA Publishing 2015 Water Science & Technology Vol.72 No.7
<P>Pharmaceuticals are very useful in treating human diseases but they are excreted to the environment sometimes in their original form or as byproducts of human metabolism. Pharmaceuticals and their metabolites have been proven by studies to be harmful to non-target ecological species and may be persistent in different water matrices. In this regard, there is an emergent need to eliminate these compounds to prevent their adverse effects on aquatic species. Biodegradation using white-rot fungi is a promising technology for the removal of recalcitrant compounds; however, products of fungal biodegradation can also be detrimental. In this novel study, we evaluated the ability of <I>Phanerochaete chrysosporium</I> to degrade amlodipine, an anti-hypertensive drug which was recently found in water systems. Analysis of amlodipine metabolites was done using quadrupole time-of-flight liquid chromatography mass spectrometry after the degradation set-up of 120 hours. Pharmaceutical degradation was seen using triple quadrupole liquid chromatography tandem mass spectrometry. Ninety-two significant metabolites (<I>P</I>-value ≤ 0.05) were significantly expressed after false discovery rate adjustment at a significance threshold of <I>q</I> = 0.05. Pyridine derivatives which were identified from samples became the basis of the proposed degradation pathway of amlodipine in this study.</P>
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De Sotto, R.B.,Medriano, C.A.D.,Salcedo, D.E.,Lee, H.,Cho, Y.,Kim, S. Academic Press 2016 Journal of Environmental Management Vol. No.
<P>In the event of a foot and mouth disease outbreak, further spread of the virus is generally prevented by culling of infected animals in burial pits. This practice may eventually lead to groundwater contamination through leaching of wastewater from the animal carcasses. Wastewater from carcass leachate often contains antibiotic resistant bacteria and genes as well as traces of pharmaceuticals, and a high nutrient content. The role of operational parameters used in activated sludge treatment of this wastewater in the spread of antibiotic resistance has not been fully understood. This study investigated the fate of tetracycline-resistant bacteria and genes in sequencing batch reactors with synthetic carcass leachate at different solid retention times. Escherichia coli DH5 alpha was used as the representative tetracycline-resistant bacteria with multiple antibiotic-resistant genes encoded in plasmid pB10. Solids retention time contributed to an increase in antibiotic resistance in SBRC (SRT = 25 days) with TRB values up to 1.25 x 107 CFU/mL which is one log higher than the influent. Microbial community analysis of the DNA samples from effluent of SBRC showed four major phyla: Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria under which are ecologically-important microbial species. It was shown that antibiotic resistance genes cannot be eliminated during treatment of synthetic carcass leachate in a lab-scale sequencing batch reactor. (C) 2016 Elsevier Ltd. All rights reserved.</P>
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Sotto, Ryan B. De,Medriano, Carl D.,Cho, Yunchul,Kim, Hyuk,Chung, In-Young,Seok, Kwang-Seol,Song, Kyung Guen,Hong, Seok Won,Park, Youngja,Kim, Sungpyo Elsevier 2017 Journal of hazardous materials Vol.339 No.-
<P><B>Abstract</B></P> <P>Antibiotics in the aquatic environment are dispersed through anthropogenic activities at low concentrations. Despite their sub lethal concentration, these biologically active compounds may still have adverse effects to non-target species. This study examined the response of adult zebrafish to 0.1mg/L concentration of clarithromycin, florfenicol, sulfamethazine, and their mixture using environmental metabolomics. Embryo and larvae of the fish were also used to assess fish embryo acute toxicity and behavior tests respectively. The fish embryo toxicity test did not show any inhibition of growth and development of the embryos after 96h of exposure to the antibiotics. Changes in swimming activity were seen in 5-dpf larvae which is believed to be correlated with the length of exposure to the compounds. Meanwhile, environmental metabolomics revealed diverse metabolites and pathways that were affected after 72h of exposure of the adult fish to sub-lethal concentration of the compounds. We found that even at low concentration of the antibiotics, behavioral and metabolic effects were still observed despite the lack of visible morphological changes. Further studies involving other aquatic organisms and bioactive compounds are encouraged to strengthen the findings presented in this novel research.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Individual and mixture of antibiotics at 0.1mg/L did not impair growth of embryos. </LI> <LI> Zebrafish larvae preferred blue over yellow regardless of antibiotic’s presence. </LI> <LI> Swimming behavior of 5-dpf larvae significantly changed relative to exposure time. </LI> <LI> Metabolites choline, guanosine, and ADP were regulated in the exposed zebrafish. </LI> <LI> Antibiotics’ mechanism of action seems to play a role in zebrafish’s metabolism. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
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De Sotto, R.,Medriano, C.,Cho, Y.,Seok, K.S.,Park, Y.,Kim, S. Academic Press 2016 Ecotoxicology and environmental safety Vol.127 No.-
<P>Recently, environmental metabolomics has been introduced as a next generation environmental toxicity method which helps in evaluating toxicity of bioactive compounds to non-target organisms. In general, efficient metabolite extraction from target cells is one of the keys to success to better understand the effects of toxic substances to organisms. In this regard, the aim of this study is (1) to compare two sample extraction methods in terms of abundance and quality of metabolites and (2) investigate how this could lead to difference in data interpretation using pathway analysis. For this purpose, the antibiotic sulfamethazine and zebrafish (Danio rerio) were selected as model toxic substance and target organism, respectively. The zebrafish was exposed to four different sulfamethazine concentrations (0, 10, 30, and 50 mg/L) for 72 h. Metabolites were extracted using two different methods (Bligh and Dyer and solid phase extraction). A total of 13,538 and 12,469 features were detected using quadrupole time-of-flight liquid chromatography mass spectrometry (QTOF LC-MS). Of these metabolites, 4278 (Bligh and Dyer) and 332 (solid phase extraction) were found to be significant after false discovery rate adjustment at a significance threshold of 0.01. Metlin and KEGG pathway analysis showed comprehensive information from fish samples extracted using Bligh and Dyer compared to solid phase extraction. This study shows that proper selection of sample extraction method is critically important for interpreting and analyzing the toxicity data of organisms when metabolomics is applied. (C) 2016 Elsevier Inc. All rights reserved.</P>
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Ryan De Sotto,Carl Medriano,Youngja Park,Sungpyo Kim 한국방재학회 2015 한국방재학회 학술발표대회논문집 Vol.14 No.-
The presence of pharmaceuticals in the environment has led to apparent toxicity with different aquatic species. Clarithromycin, for example, is used in treating respiratory tract infections, has been recently found in the surface waters and rivers which might threaten non-targeted organisms in these matrices. In this study, a model vertebrate Danio rerio (zebrafish) was exposed to 100ppb clarithromycin for 72 hours to evaluate acute toxicity through significantly affected metabolic compounds in the fish’s pathway. Metabolites obtained from q-TOF LC/MS were identified and mapped with the zebrafish’s metabolic pathway using Metlin, and KEGG respectively. 335 compounds are believed to have been significantly altered by the acute exposure of the antibiotic with the fish. The most affected pathways are ABC transporters, steroid hormone biosynthesis, arachidonic acid metabolism, purine metabolism, and biosynthesis of amino acids. With the said findings, it can be concluded that, although concentration of some pharmaceuticals may be as low as the one used in this study, its effects on the aquatic species exposed to it might be significant and should be given immediate attention
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de Andrade, Priscila Ferreira,Silva, Jesca Neftali Nogueira,Sotto-Maior, Bruno Salles,Ribeiro, Cleide Gisele,Devito, Karina Lopes,Assis, Neuza Maria Souza Picorelli Korean Academy of Oral and Maxillofacial Radiology 2017 Imaging Science in Dentistry Vol.47 No.3
Purpose: The classification of impacted maxillary third molars(IMTMs) facilitates interdisciplinary communication and helps estimate the degree of surgical difficulty. Thus, this study aimed to develop a 3-dimensional classification of the position and depth of impaction of IMTMs and to estimate their prevalence with respect to gender and age. Materials and Methods: This cross-sectional retrospective study analyzed images in sagittal and coronal cone-beam computed tomography (CBCT) sections of 300 maxillary third molars. The proposed classification was based on 3 criteria: buccolingual position (buccal, lingual, or central), mesial-distal position (mesioangular, vertical, or distoangular), and depth of impaction (low, medium, or high). CBCT images of IMTMs were classified, and the associations of the classifications with gender and age were examined using analysis of variance with the Scheffe post-hoc test. To determine the associations among the 3 classifications, the chi-square test was used (P<.05). Results: No significant association of the classifications with gender was observed. Age showed a significant relationship with depth of impaction (P=.0001) and mesial-distal position (P=.005). The most common positions were buccal(n=222), vertical(n=184), and low (n=124). Significant associations among the 3 tested classifications were observed. Conclusion: CBCT enabled the evaluation of IMTMs in a 3-dimensional format, and we developed a proposal for a new classification of the position and depth of impaction of IMTMs.
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Priscila Ferreira de Andrade,Jesca Neftali Nogueira Silva,Bruno Salles Sotto-Maior,Cleide Gisele Ribeiro,Karina Lopes Devito,Neuza Maria Souza Picorelli Assis 대한영상치의학회 2017 Imaging Science in Dentistry Vol.47 No.3
Purpose: The classification of impacted maxillary third molars (IMTMs) facilitates interdisciplinary communication and helps estimate the degree of surgical difficulty. Thus, this study aimed to develop a 3-dimensional classification of the position and depth of impaction of IMTMs and to estimate their prevalence with respect to gender and age. Materials and Methods: This cross-sectional retrospective study analyzed images in sagittal and coronal conebeam computed tomography (CBCT) sections of 300 maxillary third molars. The proposed classification was based on 3 criteria: buccolingual position (buccal, lingual, or central), mesial-distal position (mesioangular, vertical, or distoangular), and depth of impaction (low, medium, or high). CBCT images of IMTMs were classified, and the associations of the classifications with gender and age were examined using analysis of variance with the Scheffé post-hoc test. To determine the associations among the 3 classifications, the chi-square test was used (P<.05). Results: No significant association of the classifications with gender was observed. Age showed a significant relationship with depth of impaction (P=.0001) and mesial-distal position (P=.005). The most common positions were buccal (n=222), vertical (n=184), and low (n=124). Significant associations among the 3 tested classifications were observed. Conclusion: CBCT enabled the evaluation of IMTMs in a 3-dimensional format, and we developed a proposal for a new classification of the position and depth of impaction of IMTMs.
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