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Cryogenic photoluminescence imaging system for nanoscale positioning of single quantum emitters
Liu, Jin,Davanç,o, Marcelo I.,Sapienza, Luca,Konthasinghe, Kumarasiri,De Miranda Cardoso, José,Viní,cius,Song, Jin Dong,Badolato, Antonio,Srinivasan, Kartik American Institute of Physics 2017 Review of scientific instruments Vol.88 No.2
( De Cai Jin ),( Ren Xing Liang ),( Qin Yun Dai ),( Rui Yong Zhang ),( Xue Liu Wu ),( Wei Liang Chao ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.10
Rhodococcus sp. JDC-11, capable of utilizing di-n-butyl phthalate (DBP) as the sole source of carbon and energy, was isolated from sewage sludge and confirmed mainly based on 16S rRNA gene sequence analysis. The optimum pH, temperature, and agitation rate for DBP degradation by Rhodococcus sp. JDC-11 were 8.0, 30oC, and 175 rpm, respectively. In addition, low concentrations of glucose were found to inhibit the degradation of DBP, whereas high concentrations of glucose increased its degradation. Meanwhile, a substrate utilization test showed that JDC- 11 was also able to utilize other phthalates. The major metabolites of DBP degradation were identified as monobutyl phthalate and phthalic acid by gas chromatographymass spectrometry, allowing speculation on the tentative metabolic pathway of DBP degradation by Rhodococcus sp. JDC-11. Using a set of new degenerate primers, a partial sequence of the 3,4-phthalate dioxygenase gene was obtained from JDC-11. Moreover, a sequence analysis revealed that the phthalate dioxygenase gene of JDC-11 was highly homologous to the large subunit of the phthalate dioxygenase from Rhodococcus coprophilus strain G9.
Liu, Ying,Zhou, Yan-Dong,Xiao, Yu-Li,Li, Ming-Hua,Wang, Yu,Kan, Xuan,Li, Qiu-Ying,Lu, Jian-Guang,Jin, De-Jun Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.7
Background: To examine the expression of cysteine-rich 61 (Cyr61/CCN1) protein in laryngeal squamouscell carcinoma (LSCC) tissues, and its relationship with the tumor epithelial-mesenchymal transition (EMT), invasion, metastasis, and prognosis. Materials and Methods: Immunohistochemistry was used to detect the expressions of Cyr61, Vimentin (Vim), and E-cadherin (E-cad) in 88 cases of LSCC tissues and 30 cases of tumor-adjacent normal tissues. Vim and E-cad were used as mesenchymal and epithelial markers, respectively, to determine the relationship between Cyr61 expression and the EMT of LSCC cells. In addition, clinical and histopathological data were combined to analyze the relationship between the positive-expression rates of Cyr61, Vim and E-cad and LSCC invasion, metastasis and prognosis. Results: In LSCC tissues, Vim expression rate was significantly higher than that of the tumor-adjacent tissues, whereas E-cad expression rate was significantly lower than that of the tumor-adjacent tissues. The Vim expression rate was significantly higher in stages T3 and T4 than in stages T1 and T2 LSCC tissues, whereas E-cad expression rate was significantly lower in stages T3 and T4 than in stages T1 and T2 LSCC tissues. Compared to the group without lymph node metastasis, the Vim expression rate was significantly higher and the E-cad expression rate was significantly lower in the group with lymph node metastasis. The expression rate of Cyr61 was significantly higher in LSCC tissues than in the tumor-adjacent normal tissues. In addition, the Cyr61 expression rate was higher in stages T3 and T4 than in stages T1 and T2 LSCC, and higher in the group with lymph node metastasis than in the group without lymph node metastasis. The Vim expression rate was significantly higher in the Cyr61 positive group than in the Cyr61 negative group, whereas the E-cad expression rate was significantly higher in the Cyr61 negative group than in the Cyr61 positive group. Survival analysis indicated that survival rates of Cyr61 positive, Vim positive and E-cad negative groups were significantly lower than that of Cyr61 negative, Vim negative and E-cad positive groups, respectively. Conclusions: Cyr61 expression is closely associated with LSCC invasion and lymph node metastasis. Overexpression of Cyr61 may induce EMT and therefore leads to LSCC invasion and metastasis and poor prognosis. Cyr61 may become a new maker for clinical prediction of LSCC invasion and metastasis and a new target for LSCC treatment.
Jin Zou,Qi‑Jie Zhai,Fang‑Yu Liu,Ke‑Ming Liu,De‑Ping Lu 대한금속·재료학회 2018 METALS AND MATERIALS International Vol.24 No.6
A rotating magnetic field (RMF) was applied in the solidification process of Cu-8Fe alloy. Focus on the mechanism of RMFon the solid solution Fe(Cu) atoms in Cu-8Fe alloy, the influences of RMF on solidification structure, solute distribution, andmaterial properties were discussed. Results show that the solidification behavior of Cu–Fe alloy have influenced through thechange of temperature and solute fields in the presence of an applied RMF. The Fe dendrites were refined and transformed torosettes or spherical grains under forced convection. The solute distribution in Cu-rich phase and Fe-rich phase were changedbecause of the variation of the supercooling degree and the solidification rate. Further, the variation in solute distributionwas impacted the strengthening mechanism and conductive mechanism of the material.
The Achievements and Prospects of Forage Breeding in China
Jin-Feng Yun,Lan Yun,De-Fu Liu 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08
The main develop stages of forage breeding in China was reviewed. The achievements in forage germplasm collection, preservation and evaluation were briefed. Registration varieties were classified. The improvement of forage breeding methods and techniques were summarized. Finally, make prospects on forage breeding of China.
MR T1 Image Segmentation of a Prostate Based on Distance Regularized Level Set Evolution
Yong-de Zhang,Jing-chun Peng,Gang Liu,Jin-gang Jiang,Yan-jiang Zhao 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.7
Prostate segmentation from MRI is a necessary first step and plays an important role in clinical decision making process. A MR T1 Image segmentation method of the prostate based on distance regularized level set evolution (DRLSE) is proposed. To smooth the prostate image to reduce the noise, a preprocessed prostate image with Gaussian kernel and get an edge indicator is convolved. We construct an energy function with a distance regularization term and an external energy term containing the edge indicator and minimize it by solving the gradient flow which can be implemented with a finite difference scheme. In segmentation experiments, the impact of parameters λ , μ , α and ε in DRLSE model on the image segmentation is analyzed, and an optimal value of these parameters is given. The experiment results confirm the effectiveness of the MRI segmentation method of prostate for different situation of different patients.
Study on Springback Properties of Different Orthodontic Archwires in Archwire Bending Process
Jiang Jin-gang,Wang Zhao,Zhang Yong-de,Jiang Ji-xiong,Niu Suo-liang,Liu Yi 보안공학연구지원센터 2014 International Journal of Control and Automation Vol.7 No.12
The archwire bending is one of processes the most frequently used in the orthodontic treatment. Furthermore, the springback of sheet metal, which is defined as elastic recovery of the part during unloading, should be taken into consideration so as to produce formed archwire within acceptable tolerance limits. In this paper, the springback angle of different alloy archwires (including NiTi alloy wire, Beta-Ti alloy wires, Chinese stainless steel wires, and Australian stainless steel wires) were performed by the theoretical calculation based on large deformation theory and the finite element analysis. A series of numerical simulations has been conducted for the bending test, which physically simulates the actual bending of alloy archwire with a robotic apparatus. The finite element analysis of springback is shown to be very consistent with the theoretical calculation results.
Comparison of Three Kinds of Explants in Culture of Russian Wildrye Grass
Lan Yun,Jin-Feng Yun,De-Fu Liu 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08
Thee kinds of explants, young spikes, immature and mature embryos, was compared in callus induction, embryogenic callus formation, differentiation and plant regeneration of Russian wildrye. Callus induction efficient of young spikes is highest. The imature embryos formed more somatic embryogenesis, and with highest frequency of embryogenic callus.
Isolation and Characterization of GSTs Gene Homology Sequences from Agropyron mongolicum Keng
Yan Zhao,Jin-feng Yun,De-Fu Liu 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08
In this study, GSTs gene homology fragment from Mongolian wheatgrass(Agropyron mongolicum Keng) was isolated using homology-based method. one partials gene sequences have been obtained by reverse transcription polymerase chain reaction (RT-PCR). Sequence analysis using Software of DNAman and DNAuser etc, and showed that the cDNA sequences was 344 base pairs, encoding 62 amino acids. The partial gene had C-terminal conserved domains of substrate binding pocket (H-site) of GSTs superfamily. Homology comparison with GSTs gene amino acid sequences in other plants showed that it was 91% identical to 19E50 of wheat, 90% to pk0078 and 88% to gstf6. It was named as MwGSTs.