보충사료 급여수준이 엘크 수사슴의 생산성 및 방목강도에 미치는 영향

이진욱,이상훈,이성수,전다연,김성우,윤영식,김상우,박형수,김관우,Lee, Jinwook,Lee, Sang Hoon,Lee, Sung Soo,Jeon, Dayeon,Kim, Sung Woo,Yun, Yeong Sik,Kim, Sang Woo,Park, Hyung Soo,Kim, Kwan Woo 한국초지조사료학회 2019 한국초지조사료학회지 Vol.39 No.2

본 연구는 엘크 수사슴의 방목 시 보충사료 급여수준에 따른 생산성 변화와 적정 방목강도 구명을 통하여 초지활용성을 향상시키고 사슴 방목에 대한 기초자료를 제공하기 위하여 수행되었다. 공시가축은 평균체중 195kg의 2년생 엘크(Cervus canadensis) 수사슴 15두를 공시하였으며, 보충사료 급여수준에 따라 3개의 처리구(체중의 1.0%(T1), 체중의 1.5%(T2), 체중의 2.0%(T3))에 완전임의배치를 하였다. 실험기간동안 건물섭취량과 일당증체량은 보충사료 급여수준이 높을수록 유의적으로 높게 나타났다. 월별 건물섭취량과 증체량은 봄부터 여름까지 증가하다 가을로 들어서면서 감소하는 일반적인 사슴의 섭식패턴과 유사하게 나타났다. 그러나 뿔 성장기 보충사료 급여수준이 낮을 경우 녹용성장기에 성장지연의 문제가 발생하는 것으로 나타났다. 녹용생산성의 경우 영양수준과의 연관성이 낮은 것으로 나타났다. 방목초지의 사초생산성은 5월에 가장 높았고 8월 이후 급격히 감소하는 것으로 나타났으며, 산야초를 활용한 산지방목에 비해서 조단백질 함량이 높게 나타났다. 엘크 수사슴의 월별 적정 방목강도는 사초생산성이 높은 5~6월에 보충사료 급여수준에 따라 38~59 두/ha로 높게 나타났고, 7월 이후에는 13~32 두/ha로 낮게 나타났다. 또한, 보충사료 급여수준이 증가할수록 사초섭취량이 감소하여 방목강도가 높아지는 것으로 나타났다. 본 연구의 결과로 엘크 수사슴의 방목 시 녹용성장기 성장지연을 예방하고 방목 시 안정적인 증체량을 유지하기 위해서는 보충사료를 체중의 1.5%이상 급여하는 것이 필요하다고 사료되며, 초지의 활용성을 향상시키기 위해서 사초 생산성을 고려하여 적정 방목강도의 조절이 필요한 것으로 판단된다. This study was conducted to investigate the effect of supplementary feeding levels on livestck and forage productivity and grazing intensity in Elk stags (Cervus canadensis). A fifteen 2-year-old Elk stags about 195 kg were randomly assigned to one of three dietary treatments (five animals per treatment). The dietary treatments consisted of a feeding concentrate of 1.0% of body weight (T1), 1.5% of body weight (T2) and 2.0% of body weight. Total dry matter intake (TDMI) was increased with increased with an increasing supplementary feeding levels. Average daily gain (ADG) were significantly increased with an increasing supplementary feeding levels (p<0.05) and reached a maximum on July and was lower in spring than autumn. The velvet antler production was no differences among treatment groups. Forage productivity of pasture and crude protein content were highest on May and decreased thereafter, however, crude fiber content was the reversed. The grazing intensity of Elk stags was increased in spring (38 to 59 head per ha) than summer and autumn (13 to 32 head per ha). The average grazing intensity of Elk stags ranged from 21 to 34 head per ha, which is affected by supplementary feeding levels. This result suggests that feeding supplementary diet at 1.5 % of body weight was needed to maintain the stable wight gain in antler growing periods and control the proper grazing intensity of Elk deer stags.

Control Effect of Tomato Bacterial wilt caused by Ralstonia solanacearum Using Water Extract of Spent Mushroom Media of Lentinula edodes

Sang Yeob Lee,Jeong Jun Kim,Ji Hee Han,Dayeon Kim 한국농약과학회 2016 한국농약과학회 학술발표대회 논문집 Vol.2016 No.4

Biological Control of Gom-chwi (Ligularia fischeri) Phytophthora Root Rot with Enterobacter asburiae ObRS-5 to Suppress Zoosporangia Formation and Zoospores Germination

Dayeon Kim,Sang Yeob Lee,Seong Ho Ahn,Ji Hee Han,Jin Woo Park 한국식물병리학회 2020 Plant Pathology Journal Vol.36 No.3

Gom-chwi (Ligularia fischeri) is severely infected with Phytophthora drechsleri, the causal organism of Phy- tophthora root rot, an economically important crop disease that needs management throughout the cultiva- tion period. In the present study, Phytophthora root rot was controlled by using bacterial isolates from rhizo- sphere soils collected from various plants and screened for antagonistic activity against P. drechsleri. A total of 172 bacterial strains were isolated, of which, 49 strains showed antagonistic activities by dual culture assay. In the seedling assay, six out of the 49 strains showed a predominant effect on suppressing P. drechsleri. Among the six strains, the ObRS-5 strain showed remarkable against P. drechsleri when treated with seed dipping or soil drenching. The ObRS-5 strain was identified as Enterobacter asburiae based on 16S ribosomal RNA gene sequences analysis. The bacterial cells of E. asburiae ObRS-5 significantly suppressed sporangium formation and zoospore germination in P. drechsleri by 87.4% and 66.7%, respectively. In addition, culture filtrate of E. asburiae ObRS-5 also significantly inhibited sporangium formation and zoospore germination by 97.0% and 67.6%, respectively. Soil drenched bacte- rial cells, filtrate, and culture solution of E. asburiae ObRS-5 effectively suppressed Phytophthora root rot by 63.2%, 57.9%, and 81.1%, respectively. Thus, E.asburiae ObRS-5 could be used as a potential agent for the biological control of Phytophthora root rot infecting gom-chwi.

재래흑염소의 형태적 특징 및 생육특성

이상훈(Sang-Hoon Lee),이진욱(Jinwook Lee),전다연(Dayeon Jeon),김승창(Seungchang Kim),김관우(Kwan-Woo Kim) 한국산학기술학회 2019 한국산학기술학회논문지 Vol.20 No.8

본 연구는 우리나라 고유 재래흑염소 유전자원인 당진계통, 장수계통 및 통영계통의 특성을 조사하였다. 재래흑염소 형태적 특징을 조사한 결과 장수계통과 통영계통의 모색은 전신 흑색을 나타내었으며 당진계통은 흑색 또는 흑갈색으로 긴 털의 특징이 뚜렷하게 나타났다. 일부 개체에서 이모색이 출현하였고 당진계통, 장수계통 및 통영계통 순으로 그 비율이 높게 조사되었다. 재래흑염소의 계통별 육염과 뿔의 유무를 조사한 결과 통영계통에서만 육염이 출현하지 않았으며 당진계통의 암염소와 숫염소에서 각각 1개체씩 무각의 특성을 보였다. 또한 귀의 길이와 꼬리의 길이는 암염소와 숫염소가 거의 비슷한 경향을 보였으나 뿔의 길이와 수염의 길이는 숫염소가 암염소에 비해 긴 것으로 조사되었다. 재래흑염소의 체중을 조사한 결과 생시체중은 평균 2 kg이며, 숫염소가 암염소 보다 조금 높은 경향을 보였으며 3개월령의 이유 시 체중은 모든 계통에서 숫염소가 암염소 보다 조금 높았으며 12개월령의 체중도 암염소가 계통별 16.5~19.3kg, 숫염소가 18.3~22.2 kg으로 높게 조사되었다. 재래흑염소의 체형을 조사한 결과 계통별 체장과 체고는 숫염소가 암염소보다 약간 컸으며, 흉폭과 흉위는 계통 및 성별간의 차이가 나타나지 않았다. 본 연구 결과는 재래종 염소와 교잡종 염소를 구분할 수 있는 기준 마련과 나아가 재래흑염소 개량을 위한 기초자료로 이용될 것이다. This study aims to provide basic data for investigating the phenotypic traits and growth characteristics of Korean native black goats. The Jangsu and Tongyoung strains were shown to have black hair, while the Dangjin strain was shown to have black or dark brown long hair. Only the Tongyeong strain was found to have no wattle and one goat each from the females and males of the Dangjin strain was found to have no horns. The lengths of the ears and tails were similar for the female and male goats, while the lengths of horns and beards were longer in the male goats. The birth weight was 2 kg on average, with that of male goats tending to be slightly heavier than that of the females. Weights at the weaning period were found to be slightly heavier for the males. Also, at 12 months, the weights of the male goats by strain were shown to be heavier than that of the females. The body length and depth by strain were found to be higher for the male goats, while no differences in the chest width and girth between the strains or gender were found. These findings are expected to be used as a basis for differentiating between native and crossbred Korean black goat strains and for improving Korean native black goats.

유아의 실행기능이 또래유능성에 미치는 영향: 유아 놀이성의 매개효과

조다연(DaYeon Cho),성지현(Jihyun Sung) 한국아동학회 2020 아동학회지 Vol.41 No.4

Objectives: The purpose of this study was to examine the associations among executive function (EF), playfulness, and peer competence of 4, 5, and 6-year-old children, as well as the impacts of EF on peer competence. In addition, this study attempted to verify whether children’s playfulness has a mediating effect on the relationship between EF and peer competence. Methods: Study participants were 94 children from daycare centers and kindergartens in Gyeonggido. The three EF subcategories (working memory, inhibition, and shifting) were measured using the Digit Span task, the Day-Night task, and the Dimensional Change Card Sort (DCCS). Playfulness was measured using the Korean version of Childrens Playfulness Scale (CPS), and peer competence was measured using the Peer Competence Scale. The mediating effect of playfulness was confirmed using hierarchical multiple regression analysis. Results: Study results revealed the following: (1) Significant differences were seen in childrens EF according to age groups; however, there were no significant difference in playfulness and peer competence between the age groups. (2) After controlling for age, positive correlations were shown among EF, playfulness, and peer competence. (3) Playfulness fully mediated the relationship between EF and peer competence. Conclusion: This study confirms that children’s playfulness is an essential factor both forming and maintaining successful peer relationships, and makes clear the necessity of developing and implementing approaches for cultivating children’s playfulness in order for them to create and maintain successful peer relationships.

Development of pound cake with Alaska pollack roe and their nutritional and antioxidant properties

In Sang Kwon,Dayeon Lee,Jae Hee An,Min Kyo Kim,Min Soo Heu 한국수산과학회 양식분과 2022 한국수산과학회 양식분과 학술대회 Vol.2022 No.10

Prediction and conformity of nutritional properties of pound cake doughs with Alaska pollack roe added using big data on nutritional ingredients

In Sang Kwon,Dayeon Lee,Jae Hee An,Min Kyo Kim,Jin-Soo Kim,Min Soo Heu 한국수산과학회 양식분과 2022 한국수산과학회 양식분과 학술대회 Vol.2022 No.10

혈청과 난포액 및 성선자극호르몬 첨가가 염소 난자의 체외성숙에 미치는 영향

이상훈(Sang-Hoon Lee),전다연(Dayeon Jeon),이진욱(Jinwook Lee),이성수(Sung-Soo Lee),김승창(Seungchang Kim),김찬란(Chan-Lan Kim),김관우(Kwan-Woo Kim) 한국산학기술학회 2019 한국산학기술학회논문지 Vol.20 No.9

본 연구는 염소 난자의 체외배양 시 혈청과 호르몬 첨가의 효과를 알아보기 위하여 체외성숙 효율, 유전자발현 및 체외수정 후 배발달율을 조사하였다. 본 실험에서는 기본 체외성숙 배양액에 10% gBS, 10% gFF를 첨가하여 혈청유무에 따른 효과와 FSH를 조합하여 체외성숙 효율을 조사한 결과, 처리구 모두 대조구보다 유의적으로 높은 성숙률을 보였다. 특히 gBS와 FSH를 조합하였을 때 처리구 중에서도 77.8%로 유의적으로 가장 높은 체외성숙 결과를 보였다. 또한, 각 처리구로부터 얻은 성숙 난자를 이용하여 체외수정 배발달 효율을 조사한 결과 모두 유의적으로 향상되었으며 특히 gBS와 gFF에 FSH를 혼합 처리한 두 그룹이 우수한 결과를 보였다. 난자의 성숙과 관련된 BMP15와 GDF9의 mRNA 발현량을 비교한 결과, 처리구간 특이점이나 유의적 차이가 나타나지 않았다. 본 연구의 결과 염소 난자의 체외성숙에 관한 추가적인 실험이 필요할 것으로 생각되나, gBS, gFF 및 FSH 첨가가 성숙 효율 향상에 도움이 됨을 알 수 있었다. This study investigated the effects of goat blood serum (gBS), goat follicular fluid (gFF) and gonadotropin (FSH) on the in vitro maturation, fertilization and development of Korean native black goat oocytes. Our results indicate that the gBS combined with FSH treated group showed significantly higher maturation rate than the other groups. Furthermore, blastocyst formation rate was significantly increased in all treated groups, and gBS and gFF combined with FSH treated groups were higher than other groups. However, gene expression levels of BMP15 and GDF9 in COC, both oocyte maturation related genes, remained unaffected after 24 h maturation. The results of the present study indicate that supplementation of the maturation medium with gBS, gFF and FSH is efficacious in improving the in vitro maturation, fertilization and development of Korea native black goat oocytes.

Grazing Behavior and Forage Selection of Goats (Capra hircus)

Lee, Sang-Hoon,Lee, Jinwook,Chowdhury, M.M.R.,Jeon, Dayeon,Lee, Sung-Soo,Kim, Seungchang,Kim, Do Hyung,Kim, Kwan-Woo The Korean Society of Grassland and Forage Science 2019 한국초지조사료학회지 Vol.39 No.3

The normal feeding approach of goats might be due to their precise anatomical and physiological characteristics of entity, which permit them to be highly selective, to eat legume silages and wild green grass. This review has been designed to consider the grazing behavior, fodder selection, and feed composition of goats. Various herbs and corns consumed by goats have numerous nutritive resources. Based on the general herbaceous intake activities and behavior of goats, they prefer wild grass such as grass grown in the steep hills than soft grass. Because the digestion capacity of cellulose feed has higher digestion level compared to other non-ruminants within rumen and it is advantageous to use wild forest or mountain grass which comprises high proportion of cellulose feed for goat. In South Korea, there are abundant feed resources for goats because of occupying large areas of mountains. Thus, goat production and feeding costs could be reduced if plants are used from the wild forest as a feed for goats relative to grassland grazing. Also, it is expected to contribute in improvement of goat farming with harmonious relationship between the grassland and wild forest while satisfying animal welfare and physiological desires of livestock.

Stent containing CD44-targeting polymeric prodrug nanoparticles that release paclitaxel and gemcitabine in a time interval-controlled manner for synergistic human biliary cancer therapy

Yun, Dayeon,Kim, Hyun-Ouk,Son, Hye Young,Choi, Yuna,Noh, Ilkoo,Lim, Jong-Woo,Kim, Jihye,Chun, Haejin,Park, Geunseon,Lee, Dong Ki,Jang, Sung Il,Jang, Eunji,Huh, Yong-Min,Haam, Seungjoo Royal Society of Chemistry 2017 Journal of Materials Chemistry B Vol. No.

<P>The use of drug-eluting stents (DESs) is a promising strategy for non-vascular diseases, especially human biliary cancer. However, the implementation of DESs suffers from two major obstacles: the side effects of drugs and the difficulty of controlling the drug release. These problems can be overcome if the stent elutes targeting nanoparticles that release drugs at time intervals that are dictated by the mechanisms of those drugs. We designed temporally controlled polymeric multi-prodrug nanoparticles (TCMPNs) that can be eluted from stents comprising polyurethane (PU) nanofiber as a polymeric matrix and paclitaxel (PTX)-loaded, CD44-targeting, hyaluronic acid-conjugated poly(lactic-<I>co</I>-glycolic acid) and gemcitabine (GEM) (P-H-G). TCMPNs enable two different types of drugs to be released temporally; PTX is released first owing to the collapse of the structure in the endosomes, and GEM, which induces synergistic anticancer activities, is hydrolyzed from P-H-G later in response to low pH. Embedded in the PU nanofiber, the TCMPNs demonstrate low initial burst behavior and sustainable release of the prodrug <I>in vitro</I>. Furthermore, TCMPN-eluting stents (TESs) exhibit continuous synergistic efficacy as available targeted cellular uptake prodrug delivery systems in tumor-bearing mice. These results demonstrate that this technology will open up cancer therapy by combining localized delivery and functional multi-drug-loaded nanoparticles.</P>