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Das, Debabrata,Rai, Avdhesh Kumar,Kataki, Amal Chandra,Barmon, Debabrata,Deka, Pankaj,Sharma, Jagannath Dev,Sarma, Anupam,Shrivastava, Sushruta,Bhattacharyya, Mouchumee,Kalita, Apurba Kumar,Sharma, Mo Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2
Background: Persistent infection of one or more of about 15 high-risk human papillomaviruses (HR-HPVs), most commonly HPV types 16/18, has a significant role in cervical cancer initiation and progression. There are limited data available from north-east India about HPV prevalence though this region has high incidence rates of cervical cancer. The aim of this study was to investigate the HPV genotypes prevalent in cervical cancer patients of north-east India. Materials and Methods: We analyzed 107 cervical cancer patient samples. Nested multiplex PCR assays were employed for detection of 13 high risk and 5 low risk HPV types. Results: HPV was confirmed in 105 samples. The presence of 6 'carcinogenic' HPV types, HPV-16 (88%), -18 (15%), -31(4%),-45 (3%), -59 (4%), -58(1%), and one non carcinogenic, HPV-6/11 (6%), was recorded. Among various demographic and clinical factors only tumour stage showed a statistically significant association with HPV type infection (P=0.019). Conclusions: We suggest that the most prevalent genotype is HPV-16 followed by HPV-18 in cervical carcinoma patients of the north-eastern region of India. Advanced tumour stage may be associated with increased possibility of harbouring multiple HPV genotypes.
Hybrid Capture 2 Assay Based Evaluation of High-Risk HPV Status in Healthy Women of North-East India
Rai, Avdhesh Kumar,Das, Debabrata,Kataki, Amal Chandra,Barmon, Debabrata,Aggarwal, Dippy,Deka, Pankaj,Shrivastava, Sushruta,Sharma, Jagannath Dev,Sarma, Anupam,Baruah, Upasana,Sharma, Mousumi Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2
Background: High risk HPV (HR-HPV) testing has been recommended as an effective tool along with cytology screening in identification of cervical intraepithelial lesions (CINs) and prevention of their progress towards invasive cervical cancer. The aim of this study was to assess the HR-HPV DNA status by Hybrid Capture 2 (HC2) assay in healthy asymptomatic women of North-East India. Materials and Methods: This study examined cervical cell samples of forty three (n=43) healthy women by HC2 assay. A High Risk HPV DNA kit (Qiagen) was used which can detect 13 high risk HPV types: 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 and 68. Results: The mean relative light units (RLU) for samples was in the range of 141-5, 94, 619. HR-HPV DNA was confirmed in 16% (7/43) of participant women samples. Among demographic and clinical parameters, menstrual irregularity (p=0.039) and infection history (p=0.028) has shown statistically significant differences between the HR-HPV-positive and negative groups. In the HR-HPV positive group, two women were confirmed for CINs after colposcopy and histopathologic examination. Conclusions: We suggest that there may be an association between irregular menstruation and infection history of the urogenital tract with HR-HPV DNA prevalence in North-East Indian asymptomatic women. HC2 assay can be a valuable tool for HR-HPV screening.
Yutika Narzary,Sandeep Das,Arvind Kumar Goyal,Su Shiung Lam,Hemen Sarma,Dolikajyoti Sharma 한국식품연구원 2021 Journal of Ethnic Foods Vol.8 No.-
The cleaner production of biomass into value-added products via microbial processes adds uniqueness in terms of food quality. The microbe-mediated traditional process for transforming biomass into food is a sustainable practice in Asian food industries. The 18 fermented fish products derived through this process as well as the associated micro-flora and nutritional composition have been focused. This review aims to update the process of green conversion biomass into value-added food products for a more sustainable future. Fish products are classified based on the substrate and source of the enzymes used in fermentation, which includes the three types of technology processing discussed. According to the findings, these fermented fish contain a plethora of beneficial microbiota, making them a valuable source of probiotics that may confer nutritional and health benefits. Bacillus (12 products), Lactobacillus (12 products), Micrococcus (9 products), and Staphylococcus (9 products) were the most common bacterial genera found in 18 fermented fish products. Consuming fermented fish products is beneficial to human health due to their high levels of carbohydrate, protein, fat, and lactic acid. However, biogenic amines, which are produced by certain bacteria as a by-product of their catabolic activity, are a significant potential hazard in traditionally fermented fish.
Meinam Annebushan Singh,Koushik Das,Deba Kumar Sarma 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.1
Electrical discharge machining or Wire electrical discharge machining have proven to be an alternate mean for machining nonconducting materials to a certain extent. Application of assisting electrode over the surface of the non-conducting material plays an important role in successful machining. A numerical model is formulated to understand the effect of assisting electrode and its thickness on material removal characteristics. The modelling is carried out for a 2-D axis-symmetric geometry using Finite element and Finite volume method. The work piece is subjected to a Gaussian distribution of heat flux through an assisting electrode layer. The thermal analysis show satisfactory results for machining of non-conducting materials by wire electrical discharge machining process. An increase in the assisting electrode thickness has resulted in higher heat dissipation rate along the radial direction as compared to the vertical depth. The developed solver has been validated with the experimental and numerical results of previous literature.