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M. Angela A. Meireles,Camila G. Pereira,Patr?ia F. Leal,Daisy N. Sato 한국식품영양과학회 2005 Journal of medicinal food Vol.8 No.4
In the present work the antioxidant and antimycobacterial activities were determined for extracts from Taber-naemontana catharinensis. The extracts’ global yields were obtained using supercritical CO2 plus cosolvent. The cosolventsethanol, isopropyl alcohol, methanol, and water and their mixtures were used. The extracts were fractionated and analyzed bythin-layer chromatography and gas chromatography/flame ionization detection. The antimycobacterial activity was measuredagainst Mycobacterium tuberculosis, Mycobacterium avium, and Mycobacterium kansasii. The antioxidant activity was de-termined by the coupled reaction of .-carotene and limonene acid. The average global yield was approximately constant(2.4. 0.1%) for the alcoholic cosolvents and significantly larger (15. 1%) for the cosolvent water and its alcoholic mix-tures. The content of alkaloids in the extracts was strongly affected by the cosolvent. The antioxidant activity of the extractsranged from 53% to 95%. The highest antimycobacterial activity was detected in the alkaloidal fraction (minimum inhibitoryconcentration. 128 .g/mL), while the lowest was verified in the aqueous fraction (minimum inhibitory concentration . 512.g/mL).