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- 저자 : Dabwan Khaled Homoud Mohammed (검색결과 2 건)
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Yazeed A. Al-Sherikh,Lakshmana K. Gowda,M. Marie Mohammed Ali,James John,Dabwan Khaled Homoud Mohammed,Pradeep Chikkabidare Shashidhar 대한진단검사의학회 2014 Annals of Laboratory Medicine Vol.34 No.3
Background: Streptococcus pneumoniae causes life-threatening infections such as men- ingitis, pneumonia, and febrile bacteremia, particularly in young children. The increasing number of drug-resistant isolates has highlighted the necessity for intervening and control- ling disease. To achieve this, information is needed on serotype distribution and patterns of antibiotic resistance in children. Methods: All cases of invasive pneumococcal disease (IPD) in children aged less than 15 yr recorded at King Khalid University Hospital, King Saud University, Riyadh, Saudi Arabia, were reviewed for serotyping and antibiotic susceptibility. Isolates were collected from 78 consecutive patients with IPD between 2009 and 2012. All collected isolates were sub- jected to serotyping by co-agglutination, sequential multiplex PCR, and single PCR se- quetyping as previously described. Results: The most frequently isolated IPD serotypes were 23F, 6B, 19F, 18C, 4, 14, and 19A, which are listed in decreasing order and cover 77% of total isolates. The serotype coverage for the pneumococcal conjugate vaccine (PCV)7, PCV10, and PCV13 was 77%, 81%, and 90%, respectively. Results from sequential multiplex PCR agreed with co-agglu- tination results. All serotypes could not be correctly identified using single PCR sequetyp- ing. Minimum inhibitory concentration showed that 50 (64%) isolates were susceptible to penicillin, whereas 70 (90%) isolates were susceptible to cefotaxime. Conclusions: The most common pneumococcal serotypes occur with frequencies similar to those found in countries where the PCV has been introduced. The most common sero- types in this study are included in the PCVs. Addition of 23A and 15 to the vaccine would improve the PCV performance in IPD prevention.
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Yazeed A. Al-Sheikh,Lakshmana K. Gowda,M. Marie Mohammed Ali,James John,Dabwan Khaled Homoud Mohammed,Pradeep Chikkabidare Shashidhar 대한진단검사의학회 2014 Annals of Laboratory Medicine Vol.34 No.5
Background Streptococcus pneumoniae causes life-threatening infections such as meningitis, pneumonia, and febrile bacteremia, particularly in young children. The increasing number of drug-resistant isolates has highlighted the necessity for intervening and controlling disease. To achieve this, information is needed on serotype distribution and patterns of antibiotic resistance in children. Methods All cases of invasive pneumococcal disease (IPD) in children aged less than 15 yr recorded at King Khalid University Hospital, King Saud University, Riyadh, Saudi Arabia, were reviewed for serotyping and antibiotic susceptibility. Isolates were collected from 78 consecutive patients with IPD between 2009 and 2012. All collected isolates were subjected to serotyping by co-agglutination, sequential multiplex PCR, and single PCR sequetyping as previously described. Results The most frequently isolated IPD serotypes were 23F, 6B, 19F, 18C, 4, 14, and 19A, which are listed in decreasing order and cover 77% of total isolates. The serotype coverage for the pneumococcal conjugate vaccine (PCV)7, PCV10, and PCV13 was 77%, 81%, and 90%, respectively. Results from sequential multiplex PCR agreed with co-agglutination results. All serotypes could not be correctly identified using single PCR sequetyping. Minimum inhibitory concentration showed that 50 (64%) isolates were susceptible to penicillin, whereas 70 (90%) isolates were susceptible to cefotaxime. Conclusions The most common pneumococcal serotypes occur with frequencies similar to those found in countries where the PCV has been introduced. The most common serotypes in this study are included in the PCVs. Addition of 23A and 15 to the vaccine would improve the PCV performance in IPD prevention.
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