시중에 판매되고 있는 아이스크림의 일반세균 및 대장균군 오염 실태

정다운,김영환,손종렬,변상훈 高麗大學校 倂設 保健大學 保健科學硏究所 2003 保健科學論集 Vol.29 No.1

Ice cream is a vastly consumed food enjoyed by a wide range of people. Therefore, it is easily accessible and amounts to hundreds of its kind. Ice cream is a ready-made food which could be served without undergoing cooking process and thus putting weight on its hygienic condition. And so we set out to conduct some research on the number of E-coli bactria contained in commercially sold ice creams. We applied Lactose broth method along with Desoxycholate agar method which enables us to verify the existence of E-coli bacteria and how much is contained. We focused our research on soft ice creams and aimed to seek out the degree to which they were contaminated by E-coli bacteria. 4 major distributors of soft ice cream out of 5 didn't meet the maximum allowance of E coli bacteria to be found. The number of general bacteria observed per 1 ㎖ of specimen must be held at less than 100,000, and this wasn't violated by any of the companies' samples. Yet on E-coli testing, all of K, L, B and M companies' samples respectively exceeded the permitted limits of bacteria to be detected.

디메틸포름아미드 노출 근로자의 요중 N-메틸포름아미드 분석시 GC-NPD와 GC-FID 방법간 비교

정다운,김현욱 가톨릭대학산업의학센타 산업의학연구소 1999 韓國의 産業醫學 Vol.38 No.1

This purpose of the this study was to compare the results of two analytical techniques, GC-NPD and GC-FID, of quantifying urinary NMF from 70 DMF exposed workers in synthetic resins, leather, and dye manufacturing industries. In addition, the relationship between conecntrations of airborne DMF and urinary NMF was investigated using samples collected from 12 workers. Airborne DMF was sampled using both charcoal and silicagel tubes. All urine specimens were collected both prior-to and at end-of workshifts. The results were as follows: 1. The detection limits of urinary NMF by GC-FID and GC-NPD were 0.043㎎/l and 0.009㎎/l, respectively. The recovery rates were 96.9% for the GC-NPD and 90.2% for the GC-FID methods. 2. Desorption efficiencies of DMF on Charcoal tubes were very poor with 32.96%, while those on silicagel tubes were marginally acceptable with 85.70%. 3. A statistically significant relationship between concentrations of airborne DMF and urinary NMF from 12 workers determined by GC-NPD (r=0.74) and by GC-FID (r=0.67) was observed. 4. Geometric mean concentrations of urinary NMF of the end-of-shift urines from 70 workers were 15.84㎎/g creatinine by GC-NPD and were 9.88㎎/g creatinine by GC-FID, respectivery. For the prior-to-shift samples, they were 6.05 ㎎/g creatinine by GC-NPD and 0.92㎎/g creatinine by GC-FID. These results of this study suggest that for collecting airborne DMF in the workplace, silicagel tubes should be utilized. For quantifying urinary NMF concentrations as a biological marker of DMF exposed workes, urine should be collected at the end-of-shift. Urinary NMF should be analyzed by GC-NPD because of its lower of detection and better precision than that of GC-FID.

제1유구치의 조기 상실로 인한 공간 변화에 대한 3차원적 분석

김지연,정다운,곽소연,유승은,박기태 大韓小兒齒科學會 2008 大韓小兒齒科學會誌 Vol.35 No.2

본 연구는 제1유구치의 조기 상실 시 공간 변화에 대한 분석을 위하여 3-Dimensional Laser Scanner를 이용하여 그 유용성을 평가하고자 하는 예비논문이다. 6세-8세 사이의 제1대구치가 맹출하여 확실한 교합 관계를 이루는 혼합치 열기 어린이 중 편측 제1유구치의 조기 발치가 필요한 6명의 발치 전 후의 모형을 이용하여 다음과 같은 결과를 얻었다. l. 유구치 공간변화는 상악은 실험군과 대조군 모두 현저한 감소를 보이지 않았고, 하악은 실험군에서 3명 중 2명에서 공간감소가 관찰되었다. 2. 치열궁 너비,치열궁 둘레는 초기모형과 최종모형에서 비슷한 값을 보였다. 3. 협설측 경사도 변화는 유견치에서는 상하악 모두 특별한 경향을 보이지 않았으며,제2유구치는 상악은 실험군과 대조군이 유사하였고,하악에서는 3명중 2명에서 실험군이 더 큰 설측경사도 변화를 보였다. 제1대구치는 하악에서 실험군이 더 큰 설측경사도 변화가 관찰되었다. 4. 근원심 경사도 변화 측정에서 유견치는 상하악 모두 특별한 경향이 관찰되지 않았고 제2유구치의 경우 하악에서 실험군이 더 큰 근심경사도 증가가 보였다. 제1대구치는 상악에서 실험군,대조군 모두 원심 경사도가 증가되었다. The purpose of this study was to evaluate a 3-Dimensional laser scanner for the space analysis after loss of a primary first molar. Six children with premature loss of a primary first molar were examined using study models taken before and after the extraction. The results were as follows: 1. There was no change in primary molar space after the extraction of a maxillary primary first molar However, 2 out of 3 children experienced primary molar space loss in extraction side of a mandibular primary first molar. 2. Arch width and arch perimeter showed no difference between initial and final model. 3. All primary canines did not show any changes in inclination. Maxillary primary second molars had similar changes in both extraction and control side. However, 2 out of 3 mandibular primary second molars in extraction side showed more lingual tipping compared to control side. Mandibular permanent first molars tipped more lingually in extraction side. 4. In angulation, primary canines showed nothing of significance. Mandibular primary second molars tipped more mesially in extraction side than in control side. Maxillary permanent first molars have increased distal angulation after extraction of primary first molars in both side.

A novel zebrafish human tumor xenograft model validated for anti-cancer drug screening

Jung, Da-Woon,Oh, Eun-Sang,Park, Si-Hwan,Chang, Young-Tae,Kim, Cheol-Hee,Choi, Seok-Yong,Williams, Darren R. The Royal Society of Chemistry 2012 Molecular bioSystems Vol.8 No.7

<P>The development of a relatively simple, reliant and cost-effective animal test will greatly facilitate drug development. In this study, our goal was the establishment of a rapid, simple, sensitive and reproducible zebrafish xenograft model for anti-cancer drug screening. We optimized the conditions for the cancer cell xenograft in terms of injected cell numbers, incubation temperature and time. A range of human carcinoma cell types were stained with a fluorescent dye prior to injection into the fish larvae. Subsequent cancer cell dissemination was observed under fluorescent microscopy. Differences in injected cell numbers were reflected in the rate of dissemination from the xenograft site. Paclitaxel, known as a microtubule stabilizer, dose-dependently inhibited cancer cell dissemination in our zebrafish xenograft model. An anti-migratory drug, LY294002 (phosphatidylinositol 3-kinase inhibitor) also decreased the cancer cell dissemination. Chemical modifications to increase cancer drug pharmacokinetics, such as increased solubility (17-DMAG compared to geldanamycin) could also be assessed in our xenograft model. In addition to testing our new model using known anti-cancer drugs, we carried out further validation by screening a tagged triazine library. Two novel anti-cancer drug candidates were discovered. Therefore, our zebrafish xenograft model provides a vertebrate animal system for the rapid screening and pre-clinical testing of novel anti-cancer agents, prior to the requirement for testing in mammals. Our model system should greatly facilitate drug development for cancer therapy because of its speed, simplicity and reproducibility.</P> <P>Graphic Abstract</P><P>This study reports the first rapid reproducible zebrafish human tumor xenograft model that is validated for anti-cancer drug screening. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2mb05501e'> </P>

Mixed-dye staining method for protein detection in polyacrylamide gel electrophoresis using calconcarboxylic acid and rhodamine B

Jung, Da Woon,Yoo, Gyurng Soo,Choi, Jung Kap 전남대학교 약품개발연구소 1998 약품개발연구지 Vol.7 No.1

A Unique Small Molecule Inhibitor of Enolase Clarifies Its Role in Fundamental Biological Processes

Jung, Da-Woon,Kim, Woong-Hee,Park, Si-Hwan,Lee, Jinho,Kim, Jinmi,Su, Dongdong,Ha, Hyung-Ho,Chang, Young-Tae,Williams, Darren R. American Chemical Society 2013 ACS CHEMICAL BIOLOGY Vol.8 No.6

<P>Enolase is a component of the glycolysis pathway and a “moonlighting” protein, with important roles in diverse cellular processes that are not related to its function in glycolysis. However, small molecule tools to probe enolase function have been restricted to crystallography or enzymology. In this study, we report the discovery of the small molecule “ENOblock”, which is the first, nonsubstrate analogue that directly binds to enolase and inhibits its activity. ENOblock was isolated by small molecule screening in a cancer cell assay to detect cytotoxic agents that function in hypoxic conditions, which has previously been shown to induce drug resistance. Further analysis revealed that ENOblock can inhibit cancer cell metastasis <I>in vivo</I>. Moreover, an unexpected role for enolase in glucose homeostasis was revealed by <I>in vivo</I> analysis. Thus, ENOblock is the first reported enolase inhibitor that is suitable for biological assays. This new chemical tool may also be suitable for further study as a cancer and diabetes drug candidate.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/acbcct/2013/acbcct.2013.8.issue-6/cb300687k/production/images/medium/cb-2012-00687k_0009.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/cb300687k'>ACS Electronic Supporting Info</A></P>

Novel use of fluorescent glucose analogues to identify a new class of triazine-based insulin mimetics possessing useful secondary effects

Jung, Da-Woon,Ha, Hyung-Ho,Zheng, Xuexiu,Chang, Young-Tae,Williams, Darren R. Royal Society of Chemistry 2011 Molecular bioSystems Vol.7 No.2

<P>There is an urgent need to discover new compounds that effectively treat diabetes by mimicking the action of insulin (insulin mimetics). Traditional approaches to studying anti-diabetic agents in cells are inconvenient for screening chemical libraries to identify insulin mimetics. 2-(<I>N</I>-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG) and 6-NBDG are fluorescent analogues of glucose that could be employed in screening. However, there are no published data about the use of these analogues to identify new insulin mimetics. We have developed a screening system based on 6-NBDG using 3T3-L1 adipocytes in a 96-well culture plate format. 6-NBDG was found to produce a larger signal than 2-NBDG in this screening system. 6-NBDG uptake in 3T3-L1 adipocytes was sensitive to insulin, known insulin mimetics, inhibitors of glucose transport and insulin-sensitizing compounds. To validate our screening system, a chemical library of 576 tagged, triazine-based small molecules was screened. The screening results were identical to that obtained from a commercial enzyme-based glucose assay. Two inducers of glucose uptake were shown to be non-cytotoxic and confirmed as insulin mimetic compounds by their inhibition of epinephrine-stimulated free fatty acid release from adipocytes. These novel insulin mimetics functioned at a markedly lower concentration than two widely studied insulin mimetics, zinc(<SMALL>II</SMALL>) complexes and vanadium compounds, and also showed novel, beneficial effects on endothelial cell function (a key determinant of secondary complications in diabetes). The discovery of new insulin mimetics using 6-NBDG validates the use of this probe in the development of large-scale, cell-based screening systems based on the uptake of fluorescent-tagged glucose analogues. This research should aid the development of novel strategies to discover new drugs and drug targets for combating the increasing prevalence of diabetes.</P> <P>Graphic Abstract</P><P>The authors describe a new approach to screen for anti-diabetic drugs, using 6-(<I>N</I>-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose to identify novel, triazine-based insulin mimetics. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c0mb00089b'> </P>

Antioxidant Activity and Inhibitory Effect of Adipogenesis in 3T3-L1 Adipocyte by Extracts of Sanguisorba officinalis L.

Da-Woon Jung,Yu-Hua Huang,Sun-Hyuk Im,Jeong Eun Ban,Yoon-Hee Choi,Il-Jun Kang 한국식품영양과학회 2015 한국식품영양과학회 학술대회발표집 Vol.2015 No.8

Subcutaneous Emphysema and Pneumomediastinum during Extraction of Maxillary Third Molar

Da-Woon Jung,Hyun-Joong Yoon,Sang-Hwa Lee 대한치의학회 2014 Journal of korean dental science Vol.7 No.1

Subcutaneous Emphysema and Pneumomediastinum during Extraction of Maxillary Third Molar: A Case Report

Jung, Da-Woon,Yoon, Hyun-Joong,Lee, Sang-Hwa Korean Academy of Dental Science 2014 Journal of korean dental science Vol.7 No.1

Subcutaneous emphysema and pneumomediastinum is a relatively uncommon phenomenon. It may occur secondary to dental treatment using high-speed air turbine handpieces, especially after extraction of tooth. Subcutaneous emphysema is often limited only to the areas of head and neck, but also can involve deeper structures. Thorough examination and conservative treatment of these problems are essential in preventing life-threatening complications such as airway obstruction and mediastinitis. The subject of this report is a 57-year-old woman with subcutaneous emphysema and pneumomediastinum during the extraction of maxillary third molar using high-speed air turbine handpiece. If there isn't any appropriate measure, severe complications may occur. Therefore it is important to be well-informed of proper diagnosis and treatment. This article shall present a case report with literature review.