Feasibility of biohydrogen production from Gelidium amansii

Park, J.H.,Yoon, J.J.,Park, H.D.,Kim, Y.J.,Lim, D.J.,Kim, S.H. Pergamon Press ; Elsevier Science Ltd 2011 INTERNATIONAL JOURNAL OF HYDROGEN ENERGY - Vol.36 No.21

The feasibility of hydrogen production from red algae was investigated. Galactose, the main sugar monomer of red algae, was readily converted to hydrogen by dark fermentation. The maximum hydrogen production rate and yield of galactose were 2.46 L H<SUB>2</SUB>/g VSS/d and 2.03 mol H<SUB>2</SUB>/mol galactose<SUB>added</SUB>, respectively, which were higher than those for glucose (0.914 L H<SUB>2</SUB>/g VSS/d and 1.48 mol H<SUB>2</SUB>/mol galactose<SUB>added</SUB>). The distribution of soluble byproducts showed that H<SUB>2</SUB> production was the main pathway of galactose uptake. 5-HMF, the main byproduct of acid hydrolysis of red algae causes noncompetitive inhibition of H<SUB>2</SUB> fermentation. 1.37 g/L of 5-HMF decreased hydrogen production rate by 50% compared to the control. When red algae was hydrolyzed at 150 <SUP>o</SUP>C for 15 min and detoxified by activated carbon, 53.5 mL of H<SUB>2</SUB> was produced from 1 g of dry algae with a hydrogen production rate of 0.518 L H<SUB>2</SUB>/g VSS/d. Red algae, cultivable on vast tracts of sea by sunlight without any nitrogen-based fertilizer, could be a suitable substrate for biohydrogen production.

Effect of particle-particle interaction on the bed pressure drop and bubble flow by computational particle-fluid dynamics simulation of bubbling fluidized beds with shroud nozzle

Lim, J.H.,Bae, K.,Shin, J.H.,Kim, J.H.,Lee, D.H.,Han, J.H.,Lee, D.H. Elsevier Sequoia 2016 Powder technology Vol.288 No.-

Computational particle-fluid dynamics (CPFD) simulations were carried out to determine the bed pressure drop and bubble behavior in bubbling fluidized beds produced using a shroud nozzle distributor. The fluidized bed had an internal diameter of 0.3m and height of 2.4m and was modeled using Barracuda, commercial CPFD software. The bed materials consisted of metal-grade silicon particles with d<SUB>p</SUB>, ρ<SUB>p</SUB>, and U<SUB>mf</SUB> of 150μm, 2330kg/m<SUP>3</SUP>, and 0.02m/s, respectively. The total bed inventory and the static bed height were 75kg and 0.8m, respectively. Air was used as the fluidizing gas at room temperature and atmospheric pressure and was uniformly supplied at the inlet boundary below the distributor. The superficial gas velocity was controlled in the range between 0.07 and 0.17m/s, and the restitution coefficient of the collision model and the particle normal stress parameter, which affected the particle-particle interaction, were adjusted to obtain accurate simulation data. The results of each simulation were validated by comparing the pressure drop profile to that obtained through experiments under the same conditions. The values predicted for the bed pressure drop and the bubble volume fraction changed according to the restitution coefficient in the collision model and the particle normal stress. At P<SUB>s</SUB>=5, the bed pressure drop and the bubble flow characteristics were similar to those obtained from the experimental data.

Effect of Curing Time on the Physicochemical and Sensory Properties of Beef Jerky Replaced Salt with Soy Sauce, Red Pepper Paste and Soybean Paste

Lim, H.J.,Kim, G.D.,Jung, E.Y.,Seo, H.W.,Joo, S.T.,Jin, S.K.,Yang, H.S. Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.8

This study was done to investigate the quality properties of beef jerky with soy sauce, red pepper paste, and soybean paste replacing salt. Sliced beef samples were cured in salt (control), soy sauce, red pepper paste, and soybean paste for 24 or 48 h and then dried at $70^{\circ}C$ for 8 h. Treatments showed higher final moisture content and lower $Na^+$ concentration than the control after drying for 8 h. The lightness and shear force values were lower in all treatment samples than in the control during 48 h of curing time. In particular, lower lipid oxidation was found in the jerky cured with red pepper paste than in the control. Sensory evaluation showed that color, flavor, and tenderness of jerky samples were improved by replacing salt with soy sauce, red pepper paste and soybean paste, and higher likeability scores of the beef jerky were obtained among those treatments after 48 h of curing time.

A novel canine influenza H3N2 virus isolated from cats in an animal shelter

Jeoung, H.Y.,Lim, S.I.,Shin, B.H.,Lim, J.A.,Song, J.Y.,Song, D.S.,Kang, B.K.,Moon, H.J.,An, D.J. Elsevier Scientific Pub. Co 2013 Veterinary microbiology Vol.165 No.3

The interspecies transmission of avian-origin H3N2 canine influenza virus (CIV) to dogs was first reported in 2007. The present study characterized a novel CIV H3N2 isolated from cats in an animal shelter. A comparative analysis of the deduced amino acid sequences of the A/Canine/Korea/CY009/2010(H3N2) (CY009) and A/Feline/Korea/FY028/2010 (H3N2) (FY028) strains isolated from dogs and cats, respectively, in the animal shelter identified point mutations in 18 amino acid positions within eight viral genes. Interestingly, CY009 and FY028 replicated well in specific pathogen-free embryonated chicken eggs and in mice, respectively. Mice infected with the FY028 strain exhibited significant over expression of IL-10, TNF-α, and IFN-γ (p<0.001) at 3 days postinfection. Thus, an emergency monitoring system should be developed to identify influenza mutations that occur during interspecies transmission in companion animals and for continuous public health surveillance.

한우 경제형질에 미치는 Mitochondrial DNA D-loop 영역의 염기서열 변이효과

오재돈,윤두학,공홍식,임현진,이학교,조병욱,홍기창,전광주 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.6

This study was performed to analyse the sequences of variations of mtDNA D-loop and their effects on carcass traits in Hnawoo(Korean cattle). The resulting sequences were compared with perviously published sequences for other cattle breeds(GenBank J01394). The PCR was used to amplify a total of 964 bp between nucleotide 15758 and 383 within D-loop region of mtDNA using specific primers. Twenty five polymorphic sites by nucleotide substitution were found in mtDNA of Hanwoo. The frequencies of positions at 169, 16042, 16093. 16119, 16255 and 16302 nt with high levels of sequence polymorphism were 0.891, 0.117, 0.109, 0.182, 0.197 and 0.117, respectively. The substitution effect at 169 and 16119 nt was found significant on marbling score. Also substitution effect at 169 and 16042 nt was highly significant(p<0.01) on backfat, thickness. Polymorphisrn of mtDNA sequence in D-loop region could be useful for the analysis of cytoplasmic genetic variation and associations with the other economically important traits and maternal lineage analysis in Hanwoo.

Pharmacokinetics of prothionamide in patients with multidrug-resistant tuberculosis.

Lee, H W,Kim, D W,Park, J H,Kim, S-D,Lim, M-S,Phapale, P B,Kim, E-H,Park, S K,Yoon, Y-R The Union 2009 The international journal of tuberculosis and lung Vol.13 No.9

<P>SETTING: National Masan Tuberculosis Hospital, Masan, South Korea. OBJECTIVE: To evaluate the pharmacokinetics of prothionamide (PTH) in South Korean patients with multidrug-resistant tuberculosis (MDR-TB) and to investigate whether differences in body mass index (BMI) could explain observed differences in PTH disposition. DESIGN: Seventeen patients participated in the study; all had MDR-TB and had received combination anti-tuberculosis treatment, including PTH, cycloserine, ofloxacin, para-aminosalicylic acid and streptomycin or kanamycin, for at least 2 weeks. The patients were divided into two groups based on BMI: Group A (18.5 < or = BMI<23), and Group B (BMI<18.5). Serum samples were collected over 24 h, and the plasma PTH concentration was determined by a validated high-performance liquid chromatography assay. RESULTS: After steady-state administration of PTH, the mean area under the plasma concentration-time curve from time 0 to 12 h (AUC(0-12h)) was 11.0 +/- 3.7 microg h/ml. The mean T(max) and t(1/2) were respectively 3.6 h and 2.7 h. No significant difference in PTH disposition was observed between groups A and B, except for ke and t(1/2). CONCLUSION: In the pharmacokinetic parameter estimates for PTH in MDR-TB patients during routine treatment, the pharmacokinetics of PTH did not appear to correlate with extent of emaciation in MDR-TB patients.</P>

포스터 발표 : 위 ; Helicobacter pylori에 대한 포도주(wine)의 항세균 효과

김도형 ( D. H. Kim ),노임환 ( I. H. Roe ),허재형 ( J. H. Heo ),박상현 ( S. H. Park ),남승우 ( S. W. Nam ),임창영 ( C. Y. Lim ),송일한 ( I. H. Song ),김정원 ( J. W. Kim ),명나혜 ( N. H. Myung ),신지현 ( J. H. Shin ) 대한소화기학회 2002 대한소화기학회 춘계학술대회 Vol.2002 No.-

〈목적〉 위장질환을 일으키는 H. pylori를 억제시키는 항생제가 아닌 물질들은 새로운 대체요법으로 최근 들어와서 여러 가지 식용물질들에 대해 탐색되고 있다. 본 연구에서는 포도주의 항세균 효과를 생체내,외 실험으로 연구하였다. 〈방법〉 포도주는 적포도주(프랑스산, pH 3.9, 알코올농도 12%)와 백포도주(마주앙, pH 3.22, 알콜농도 11%)를 이용하였으며 같은 pH와 같은 알코올농도의 소주(참이슬, pH 7.87, 알코올농도 22%)로 비교

Ahnak functions as a tumor suppressor via modulation of TGFβ/Smad signaling pathway

Lee, I H,Sohn, M,Lim, H J,Yoon, S,Oh, H,Shin, S,Shin, J H,Oh, S-H,Kim, J,Lee, D K,Noh, D Y,Bae, D S,Seong, J K,Bae, Y S Macmillan Publishers Limited 2014 Oncogene Vol.33 No.38

We provide detailed mechanisms of Ahnak-mediated potentiation of transforming growth factor β (TGFβ) signaling, which leads to a negative regulation of cell growth. We show that Smad3 interacts with Ahnak through MH2 domain and that Ahnak stimulates Smad3 localization into nucleus leading to potentiating TGFβ-induced transcriptional activity of R-Smad. Moreover, overexpression of Ahnak resulted in growth retardation and cell cycle arrest through downregulation of c-Myc and cyclin D1/D2. We describe results from analyses of Ahnak<SUP>−/−</SUP> mouse model expressing middle T antigen in a mammary gland-specific manner (MMTV<SUP>Tg/+</SUP>Ahnak<SUP>−/−</SUP>), which showed significantly progressed hyperplasia of mammary glands compared with MMTV<SUP>Tg/+</SUP>Ahnak<SUP>+/+</SUP>. Finally, we screened multiple human breast cancer tissues and showed that the expression of Ahnak in cancer tissues is lower than that in control tissues by 50%. Taken together, these data indicate that Ahnak mediates a negative regulation of cell growth and acts as novel tumor suppressor through potentiation of TGFβ signaling.

Effect of Anodic Gas Compositions on the Overpotential in a Molten Carbonate Fuel Cell

Lee C.G.,Kim D.H.,Hong S.W.,Park S.H.,Lim H.C. The Korean Electrochemical Society 2006 한국전기화학회지 Vol.9 No.2

Anodic overpotential has been investigated with gas composition changes in a $100cm^2$ class molten carbonate fuel cell. The overpotential was measured with steady state polarization, reactant gas addition (RA), inert gas step addition (ISA), and electrochemical impedance spectroscopy (EIS) methods at different anodic inlet gas compositions, i.e., $H_2:CO_2:H_2O=0.69:0.17:0.14\;atm\;and\;H_2:CO_2:H_2O=0.33:0.33:0.33\;atm$, at a fixed $H_2$ flow rate. The results demonstrate that the anodic overpotential decreases with increasing $CO_2\;and\;H_2O$ flow rates, indicating the anode reaction is a gas-phase mass-transfer control process of the reactant species, $H_2,\;CO_2,\;and\;H_2O$. It was also found that the mass-transfer resistance due to the $H_2$ species slightly increases at higher $CO_2\;and\;H_2O$ flow rates. EIS showed reduction of the lower frequency semi-circle with increasing $H_2O\;and\;CO_2$ flow rate without affecting the high frequency semi-circle.

Administration of vitamin D3 by injection or drinking water alters serum 25-hydroxycholecalciferol concentrations of nursery pigs

Young Dal Jang,Jingyun Ma,Ning Lu,Jina Lim,H. James Monegue,Robert L. Stuart,Merlin D. Lindemann 아세아·태평양축산학회 2018 Animal Bioscience Vol.31 No.2

Objective: Two experiments were conducted to evaluate vitamin D3 administration to nursery pigs by injection or in drinking water on serum 25-hydroxycholecalciferol (25-OHD3) concentrations. Methods: At weaning, 51 pigs (27 and 24 pigs in experiments 1 and 2, respectively) were allotted to vitamin D3 treatments. Treatments in experiment 1 were: i) control (CON), no vitamin administration beyond that in the diet, ii) intramuscular (IM) injection of 40,000 IU of vitamin D3 at weaning, and iii) water administration, 5,493 IU of vitamin D3/L drinking water for 14 d postweaning. Treatments in experiment 2 were: i) control (CON), no vitamin administration, and ii) water administration, 92 IU of d-α-tocopherol and 5,493 IU of vitamin D3/L drinking water for 28 d postweaning. The lightest 2 pigs within each pen were IM injected with an additional 1,000 IU of d-α-tocopherol, 100,000 IU of retinyl palmitate, and 100,000 IU of vitamin D3. Results: In both experiments, serum 25-OHD3 was changed after vitamin D3 administration (p<0.05). In experiment 1, injection and water groups had greater values than CON group through d 35 and 21 post-administration, respectively (p<0.05). In experiment 2, serum values peaked at d 3 post-administration in the injection groups regardless of water treatments (p<0.05) whereas CON and water-only groups had peaks at d 14 and 28 post-administration, respectively (p<0.05). Even though the injection groups had greater serum 25-OHD3 concentrations than the non-injection groups through d 7 post-administration regardless of water treatments (p<0.05), the water-only group had greater values than the injection-only group from d 21 post-administration onward (p<0.05). Conclusion: Serum 25-OHD3 concentrations in pigs increased either by vitamin D3 injection or drinking water administration. Although a single vitamin D3 injection enhanced serum 25-OHD3 concentrations greater than water administration in the initial period post-administration, a continuous supply of vitamin D3 via drinking water could maintain higher serum values than the single injection.