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RISS 인기검색어
- 검색키워드
- 저자 : Cutrignelli, Monica I. (검색결과 4 건)
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Bovera, F.,Calabro, S.,Cutrignelli, M.I.,Di Lella, T. Asian Australasian Association of Animal Productio 2002 Animal Bioscience Vol.15 No.5
Among Italian buffalo farmers, it is widely held that administering diets with high energy and protein concentrations is an effective way to increase milk production. In order to assess the validity of this opinion, we verified milk yield and physico-chemical characteristics from buffaloes that, from the $5^{th}$ month of lactation, were fed two total mixed rations (TMRs) which, given the same intake, should have led to satisfaction of protein requirements though with a slight energy deficit (diet A) or excessive amounts of energy and protein (diet B). Estimate of the energy and protein value of the diets and that of the corresponding requirements was carried out both by using two software programs derived from the Cornell Net Carbohydrate and Protein System (1992), and with the method set up by INRA researchers (1988). The results obtained show that the two diets administered did not result in significant changes to the quantity of milk produced. However, with Diet B the protein concentration in the milk was significantly (p<0.01) higher, although this was partly offset by the higher concentration (p<0.05) of non-protein nitrogen (NNP). The Group B buffaloes also showed significantly higher blood urea levels (p<0.01), with concentrations exceeding those considered physiological for lactating buffaloes. Finally, while administering Diet A the Body Condition Score (BCS) was close to 6.5 (Wagner et al., 1988), whereas in buffaloes which used Diet B it sometimes increased by over 0.5 points. As regards which of the two methods compared is more suitable for expressing dietary energy and protein value and corresponding requirements, we feel that due to the high variability in the Italian Mediterranean buffalo's milk production aptitude, it would be premature to express a judgement on methods which rest on a common scientific base and do not differ substantially.
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Nutritional Characteristics of Forage Grown in South of Benin
Musco, Nadia,Koura, Ivan B.,Tudisco, Raffaella,Awadjihe, Ghislain,Adjolohoun, Sebastien,Cutrignelli, Monica I.,Mollica, Maria Pina,Houinato, Marcel,Infascelli, Federico,Calabro, Serena Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.1
In order to provide recommendations on the most useful forage species to smallholder farmers, eleven grass and eleven legume forages grown in Abomey-Calavi in Republic of Benin were investigated for nutritive value (i.e. chemical composition and energy content) and fermentation characteristics (i.e. gas and volatile fatty acid production, organic matter degradability). The in vitro gas production technique was used, incubating the forages for 120 h under anaerobic condition with buffalo rumen fluid. Compared to legume, tropical grass forages showed lower energy (8.07 vs 10.57 MJ/kg dry matter [DM]) and crude protein level (16.10% vs 19.91% DM) and higher cell wall content (neutral detergent fiber: 63.8% vs 40.45% DM), respectively. In grass forages, the chemical composition showed a quite high crude protein content; the in vitro degradability was slightly lower than the range of tropical pasture. The woody legumes were richer in protein and energy and lower in structural carbohydrates than herbaceous plants, however, their in vitro results are influenced by the presence of complex compounds (i.e. tannins). Significant correlations were found between chemical composition and in vitro fermentation characteristics. The in vitro gas production method appears to be a suitable technique for the evaluation of the nutritive value of forages in developing countries.
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Choi, Ji Young,Iacobazzi, Rosa Maria,Perrone, Mara,Margiotta, Nicola,Cutrignelli, Annalisa,Jung, Jae Ho,Park, Do Dam,Moon, Byung Seok,Denora, Nunzio,Kim, Sang Eun,Lee, Byung Chul MDPI 2016 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.17 No.7
<P>The 18-kDa translocator protein (TSPO) levels are associated with brain, breast, and prostate cancer progression and have emerged as viable targets for cancer therapy and imaging. In order to develop highly selective and active ligands with a high affinity for TSPO, imidazopyridine-based TSPO ligand (CB256, <B>3</B>) was prepared as the precursor. <SUP>99m</SUP>Tc- and Re-CB256 (<B>1</B> and <B>2</B>, respectively) were synthesized in high radiochemical yield (74.5% ± 6.4%, decay-corrected, <I>n</I> = 5) and chemical yield (65.6%) by the incorporation of the [<SUP>99m</SUP>Tc(CO)<SUB>3</SUB>(H<SUB>2</SUB>O)<SUB>3</SUB>]<SUP>+</SUP> and (NEt<SUB>4</SUB>)<SUB>2</SUB>[Re(CO)<SUB>3</SUB>Br<SUB>3</SUB>] followed by HPLC separation. Radio-ligand <B>1</B> was shown to be stable (>99%) when incubated in human serum for 4 h at 37 °C with a relatively low lipophilicity (log<I>D</I> = 2.15 ± 0.02). The rhenium-185 and -187 complex <B>2</B> exhibited a moderate affinity (<I>K</I><SUB>i</SUB> = 159.3 ± 8.7 nM) for TSPO, whereas its cytotoxicity evaluated on TSPO-rich tumor cell lines was lower than that observed for the precursor. In vitro uptake studies of <B>1</B> in C6 and U87-MG cells for 60 min was found to be 9.84% ± 0.17% and 7.87% ± 0.23% ID, respectively. Our results indicated that <SUP>99m</SUP>Tc-CB256 can be considered as a potential new TSPO-rich cancer SPECT imaging agent and provides the foundation for further in vivo evaluation.</P>
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Fabio, Zicarelli,Calabro, Serena,Piccolo, Vincenzo,D'Urso, Simona,Tudisco, Raffaella,Bovera, Fulvia,Cutrignelli, Monica I.,Infascelli, Federico Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.1
In vivo and in vitro digestibility of 6 diets with a forage to concentrate ratio (F/C) ranging from 100 to 50:50 (diet 1: all hay, diet 2: 90:10, diet 3: 80:20, diet 4: 70:30, diet 5: 60:40, diet 6: 50:50) were investigated using 6 buffaloes in a $6{\times}6$ Latin square design. For the in vivo trial, the individual faeces of buffaloes were collected 3 times per day for 7 days. Individual pooled faeces and samples of each diet were analysed for chemical composition and insoluble acid ash (AIA) contents in order to estimate the coefficient of apparent digestibility (ADC). On the last day of the in vivo trial a sample of faeces was collected from each animal and used as inoculum for the in vitro test, using the gas production technique (IVGPT). The in vivo organic matter digestibility (ADC) rose as the percentage of concentrate increased up to the 70:30 (F/C) diet (67.01, 73.03, 78.06 and 79.05, respectively for diets 1, 2, 3 and 4); the other two diets (60:40 and 50:50 F/C) unexpectedly did not follow this trend (75.11 and 79.06, respectively for diet 5 and 6). However, these data agree with the results of the in vitro trial. The ADC was positively correlated with the dOM (p<0.001), but not with the gas production at different times; cumulative gas production recorded at the end of incubation (OMCV) showed an irregular trend and was not closely correlated to degraded OM. Estimation of in vivo digestibility from in vitro fermentation data was acceptable, despite leaving room for improvement.
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