Guidance and Counseling in Aotearoa, New Zealand, Secondary Schools: Revisiting the Issues

Ada Crowe 한국상담학회 2014 Journal of Asia Pacific counseling Vol.4 No.2

The changes in education policies in Aotearoa, New Zealand, since the 1980s have significantly affected guidance and counseling in secondary schools, leading to variations in provision and effectiveness. Issues being faced by school guidance counselors were identified in “Guidance and Counselling in New Zealand Secondary Schools: Exploring the Issues,” which represented a call for an external government authority who understood guidance and counseling and their value in schools to create national guidelines and standards for this work (Crowe, 2006). This 2014 article revisits the issues and reports on the findings and recommendations of a 2013 evaluation of guidance and counseling in Aotearoa, New Zealand secondary schools. The government requested the evaluation in response to the need to provide good guidance and counseling for young people at risk of developing mental health issues. The results and recommendations from the evaluation have highlighted the issues being faced by guidance counselors, other guidance staff, and their schools. The effectiveness of guidance and counseling in schools in Aotearoa, New Zealand will depend upon the implementation of the recommendations made in the report to the Ministry of Education, and, in particular, the review of the guidance staffing formula and the establishment of guidelines/expectations for schools/wharekura regarding the provision of guidance and counseling.

Translational Inhibition Enhances mRNA Stability of the Retinoic Acid (RA) Responsive Keratin 18 Gene Expressed by Oral Squamous Cell Carcinoma Lines but Blocks RA Dependent Stabilization

Crowe, David L.,Shuler, Charles F. Korean Academy of Oral Biology and the UCLA Dental 1997 International Journal of Oral Biology Vol.22 No.3

The keratin intermediate filament K18 has been implicated in tumor cell invasion and resistance to chemotherapy. K18 is normally not expressed in adult stratified squamous epithelium, but is reactivated in carcinomas arising from this tissue (SCCs). To understand the role of K18 in the malignant phenotype of SCCs, it is necessary to understand how expression of this gene is regulated in these cells. The vitamin A metabolite retinoic acid (R4) regulates keratin expression through both transcriptional and posttranscriptional mechanisms. RA has been shown to stabilize keratin mRNAs by as yet undefined mechanisms. Studies have shown that translation plays an important role in regulating mRNA stability. To begin to elucidate the mechanisms by which R4 exerts its posttranscriptional effects, we examined how translational inhibition affects the mRNA stability of the R4 responsive keratin 18 gene (K18) in SCC25 cells. Cycloheximide (CHX)-induced translational inhibition enhanced K18 gene expression by mRNA stabilization. CHX blocked the R4 induced stabilization of K18 transcripts, indicating that a newly synthesized or labile protein was required for R4 to mediate this effect. These studies point to the existence of multiple mRNA stabilization pathways in these cells and provide further evidence for the existence of R4 dependent factors that regulate target gene expression at the posttranscriptional level.

Optical Biopsy of Peripheral Nerve Using Confocal Laser Endomicroscopy: A New Tool for Nerve Surgeons?

Crowe, Christopher S,Liao, Joseph C,Curtin, Catherine M Korean Society of Plastic and Reconstructive Surge 2015 Archives of Plastic Surgery Vol.42 No.5

Peripheral nerve injuries remain a challenge for reconstructive surgeons with many patients obtaining suboptimal results. Understanding the level of injury is imperative for successful repair. Current methods for distinguishing healthy from damaged nerve are time consuming and possess limited efficacy. Confocal laser endomicroscopy (CLE) is an emerging optical biopsy technology that enables dynamic, high resolution, sub-surface imaging of live tissue. Porcine sciatic nerve was either left undamaged or briefly clamped to simulate injury. Diluted fluorescein was applied topically to the nerve. CLE imaging was performed by direct contact of the probe with nerve tissue. Images representative of both damaged and undamaged nerve fibers were collected and compared to routine H&E histology. Optical biopsy of undamaged nerve revealed bands of longitudinal nerve fibers, distinct from surrounding adipose and connective tissue. When damaged, these bands appear truncated and terminate in blebs of opacity. H&E staining revealed similar features in damaged nerve fibers. These results prompt development of a protocol for imaging peripheral nerves intraoperatively. To this end, improving surgeons' ability to understand the level of injury through real-time imaging will allow for faster and more informed operative decisions than the current standard permits.

Introduction to Transposons in Drosophila

Crow, James F 한국유전학회 1984 Genes & Genomics Vol.6 No.3

Episodes of high mutability were reported many years ago in Drosophila, but proved to be refractory to analysis. The work of Rhoades, McClintock, and Brink provided a solid basis for transposable elements in maize. But a deep understanding came only with the discovery and molecular analysis of transposons in microorganisms. In recent years there has been a burst of discovery of transposable elements in Drosophila. They make up as much as 10 or 20 percent of the genome. Many of them, and most of the best understood, fall into three categories: copia-like, with two direct long terminal repeats; foldback, with two long inverted terminal repeats; and P type, with short inverted terminal repeats. Some are actively mobile; the others probably are occasionally so, or have been in the past. The molecular biology of these elements has recently been reviewed by Saigo (Jap. J. Genet. 59:103, 1984). A substantial fraction of spontaneous mutations are turning out to be caused by insertions, making it seem likely that transposons have been a major cause of mutagenesis in Drosophila populations. The P factor is of particular interest because of some special properties. It causes specific phenotypes (temperature dependent sterility, mutability, chromosome breakage, and others); the rate of transposition can be exceedingly high; the rate of transposition is strictly under genotypic and cytoplasmic control, so that high or low rates of transposition can be controlled experimentally; transposition seems to be confined to the germ cells; and the molecular biology is now well understood. Cloned P factors can be injected into Drosophila eggs, where they readily integrate, bringing along whatever genes are associated with them. This opens a wealth of experimental possibilities. The P factor also exists in a modified, partially active form called Q. A similar system, the I-R system, is distinct but has many similar properties. The condition of "hybrid dysgenesis", produced when males carrying one or more P factors are mated to females without them, not only induces P-factor activity, but enhances the transposition of several other factors; so that, among the mutants induced by this system some have insertions of other elements. This implies some close similarities among the different kinds of elements, so that their relationship becomes of great interest. It is also of great interest to know how wide-spread P factors are. In situ and blot test have failed to show any material with P-homology in the great majority of Drosophila species tested, but it has been found in a few. Some developmental, mutagenic, and evolutionary aspects of transposons will be discussed.

Inhibition of Acidic Ribosomal Phosphoprotein P1 Expression Reduces Translational Efficiency and Cell Proliferation of Mouse Embryo Fibroblasts

Crowe, David L.,Cui, Xiao-Mei,Shuler, Charles F. Korean Academy of Oral Biology and the UCLA Dental 1998 International Journal of Oral Biology Vol.23 No.2

Acidic ribosomal proteins interact with multiple translation factors to regulate protein synthesis in prokaryotic and eukaryotic organisms. We have isolated and sequenced the complete cDNA of the mouse acidic ribosomal phosphoprotein P1 (mARP P1). The 495 base pair cDNA encodes a single open reading frame of 114 amino acids. Northern amalysis revealed a messenger RNA of appropriate size expressed in all tissues. Stable transfection of 3T3 cells with a vector containing an antisense mARP P1 construct reduced expression of the mRNA by 90%. Cells expressing the antisense construct did not incorporate radiolabeled methionine as efficiently as 3T3s transfected with vector alone. Cells expressing the antisense construct were also less prolifeeerative in vitro. Anlaysis of the deduced sequence and comparison to P proteins from other organisms revealed homology to the L12eILA protein in yeast. The carboxy termini of these proteins are highly conserved across species. The central alanine rich region of the mouse P1 protein likely evlived through duplications of a conserved sequence and may represent evolutionary modifications of an ancestral gene.

自然法의 探求

M. B. 크로우(Michael B. Crowe) 광주가톨릭대학교 신학연구소 1977 神學展望 Vol.- No.39

정신병과 연루된 Xq21.3 영역과 인간 태아의 뇌로부터 유래된 cDNA Library 에서 내생레트로바이러스 HERV - K LTR 의 클로닝과 계통분류

김희수,Crow, Timothy J . 한국유전학회 2001 Genes & Genomics Vol.23 No.2

Phenological properties of woody species were compared between two urban climates during 1997 and 1998. The study areas were Chungdam Park, Chungdam-dong, Kangnam-gu, Seoul (the urban center, 43 species) and Namhansansung Area, Sansung-ri, Joongbu-myon, Kwangju Gun, Kyonggi Province (the urban periphery,16 species). Distance between these sites was 13.5㎞. The differences of budding, foliation, and flowering times (1997 versus 1998) were 10.9, 3.2, and 7.4 days, respectively Species that budded and flowered earlier were strongly influenced by Nuttonson's Index (Tn) of February and March, but those with later dates were only weakly influenced. Unlike for budding and flowering times, foliation time was determined by air temperature or other factors in the leaf-growing season rather than by Tn. The Tn influence over phenology was stronger in shrubs and lianas than in trees. Phenophases in Chungdam Park appeared earlier than those in the Namhansansung area. The phenological differences between the two areas were 7.3 days in budding time, 8.3 days in foliation time, and 10.2 days in flowering time in mean values, with variations among species. Based on flowering-time data, the phenological variation between the two areas was equivalent to a 2.5°latitude difference. Budding time varied the most (20 days) in Zelkova serrata, compared with only 3 days for Prunus padus. Differences in foliation time ranged from 15 days (in Alnus hirsuta and Styrax obassia) to 0 days (P. padus). Flowering time differences were largest (24 days) in Rhododendron mucronulatum and smallest (2 days) in P. padus. One can conclude that heat pollution in the urban center in Seoul severely changed phenology, and that sensitivity to that pollution differed among plant species.

A Study on Women`s Underwear in the 1920s Using Real Materials

Sang Rye Lee,Laura Crow 한국복식학회 2005 International journal of costume and fashion Vol.5 No.2

A Study on Women's Underwear in the 1920s Using Real Materials

Lee Sang-Rye,Crow Laura The Korean Society of Costume 2005 International journal of costume and fashion Vol.5 No.2

La $d{\acute{e}}marche$ et l'objet de cette recherche sont d'analyser, psychiquement, biologiquement, les marques de $l'intersexualit{\acute{e}}$ $pr{\acute{e}}sentent$ en Occident et les elements intersexuels, binaires dans le $v{\hat{e}}tement$, et ${\acute{e}}galement$ de comprendre $l'ambigu{\ddot{i}}t{\acute{e}}$ ou encore la fusion des $identit{\acute{e}}s$ $sexu{\acute{e}}es$. qui $pr{\acute{e}}sente$ symboliquement ${\grave{a}}$ travers le $v{\hat{e}}tement$, dans la $soci{\acute{e}}t{\acute{e}}$ moderne. $Malgr{\acute{e}}$ tous les points communs et $diff{\acute{e}}rents$, la base fondamentale dans les $th{\acute{e}}ories$ de I'Occident est que tout individu est psychiquement, biologiquement en quelque sorte un intersexuel qui s'ignore, avec des variations selon l'individu. En $d{\acute{e}}finitive$, les compositions doubles des ${\acute{e}}l{\acute{e}}ments$ dans le $v{\hat{e}}tement$ occidental montraient $embl{\acute{e}}matiquement$ nos $qualit{\acute{e}}s$ doubles, masculins et $f{\acute{e}}minins$, et les trois ${\acute{e}}l{\acute{e}}ments$ semblent primordiaux pour $l'{\acute{e}}lucidation$ de la $pr{\acute{e}}disposition$ du porteur : la couleur du tissu, sa texture et la forme du $v{\hat{e}}tement$. La mode d'aujourd'hui bascule entre masculin et $f{\acute{e}}minin$. Cette fusion de la mode semble le reflet d'une tendance intersexuelle et d'une fusion du genre.

TAX RULES FOR TRANSFER PRICING : An Interjurisdictional Analysis For Pacific Rim Businesses

Kim,Jong H.,Crow, Stephen 明知大學校 經濟硏究所 1994 경영연구 Vol.10 No.1