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        Nutrient requirements and evaluation of equations to predict chemical body composition of dairy crossbred steers

        Silva Flavia Adriane de Sales,Valadares Filho Sebastião de Campos,Costa e Silva Luiz Fernando,Fernandes Jaqueline Gonçalves,Lage Bruno Corrêa,Chizzotti Mario Luiz,Felix Tara Louise 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.4

        Objective Objectives were to estimate energy and protein requirements of dairy crossbred steers, as well as to evaluate equations previously described in the literature (HH46 and CS16) to predict the carcass and empty body chemical composition of crossbred dairy cattle. Methods Thirty-three Holstein×Zebu steers, aged 19±1 months old, with an initial shrunk body weight (BW) of 324±7.7 kg, were randomly divided into three groups: reference group (n = 5), maintenance level (1.17% BW; n = 4), and the remaining 24 steers were randomly allocated to 1 of 4 treatments. Treatments were: intake restricted to 85% of ad libitum feed intake for either 0, 28, 42, or 84 d of an 84-d finishing period. Results The net energy and the metabolizable protein requirements for maintenance were 0.083 Mcal/EBW0.75/d and 4.40 g/EBW0.75, respectively. The net energy (NEG) and protein (NPG) requirements for growth can be estimated with the following equations: NEG (Mcal/kg EBG) = 0.2973(±0.1212) ×EBW0.4336(±0.1002) and NPG (g/d) = 183.6(±22.5333)×EBG−2.0693(±4.7254)×RE, where EBW, empty BW; EBG, empty body gain; and RE, retained energy. Crude protein (CP) and ether extract (EE) chemical contents in carcass, and all the chemical components in the empty body were precisely and accurately estimated by CS16 equations. However, water content in carcass was better predicted by HH46 equation. Conclusion The equations proposed in this study can be used for estimating the energy and protein requirements of crossbred dairy steers. The CS16 equations were the best estimator for CP and EE chemical contents in carcass, and all chemical components in the empty body of crossbred dairy steers, whereas water in carcass was better estimated using the HH46 equations. Objective: Objectives were to estimate energy and protein requirements of dairy crossbred steers, as well as to evaluate equations previously described in the literature (HH46 and CS16) to predict the carcass and empty body chemical composition of crossbred dairy cattle.Methods: Thirty-three Holstein × Zebu steers, aged 19 ± 1 months old, with an initial shrunk body weight of 324 ± 7.7 kg, were randomly divided into three groups: reference group (n = 5), maintenance level (1.17% BW; n = 4), and the remaining 24 steers were randomly allocated to 1 of 4 treatments. Treatments were: intake restricted to 85% of <i>ad libitum</i> feed intake for either 0, 28, 42, or 84 d of an 84-d finishing period.Results: The net energy and the metabolizable protein requirements for maintenance were 0.083 Mcal/EBW<sup>0.75</sup>/d and 4.40 g/EBW<sup>0.75</sup>, respectively. The net energy (NE<sub>G</sub>) and protein (NP<sub>G</sub>) requirements for growth can be estimated with the following equations: NE<sub>G</sub> (Mcal/kg EBG) = 0.2973<sub>(± 0.1212)</sub> × <i>EBW</i><sup>0.4336(± 0.1002)</sup> and NP<sub>G</sub> (g/d) = 183.6<sub>(± 22.5333)</sub> × EBG – 2.0693<sub>(± 4.7254)</sub> × RE, where EBW = empty body weight, EBG = empty body gain, and RE = retained energy. Crude protein (CP) and ether extract (EE) chemical contents in carcass, and all the chemical components in the empty body were precisely and accurately estimated by CS16 equations. However, water content in carcass was better predicted by HH46 equation.Conclusion: The equations proposed in this study can be used for estimating the energy and protein requirements of crossbred dairy steers. The CS16 equations were the best estimator for CP and EE chemical contents in carcass, and all chemical components in the empty body of crossbred dairy steers, whereas water in carcass was better estimated using the HH46 equations.

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        Comparative Study on the Effects of Paullinia cupana, PC-18 and Magnesium Chloride on Solid Ehrlich Tumor

        Emerson Barbosa da Silva,Felipe Ko Chen,Matheus Moreira Perez,Giuliana Petri,David Feder,Fernando Luiz Affonso Fonseca,Beatriz da Costa Aguiar Alves 한국식품영양과학회 2022 Journal of medicinal food Vol.25 No.9

        New perspectives arise in the therapeutic practice for cancer, with the objective to not only treat patients, but also improve their quality of life. Guarana, a plant from Brazilian Amazon presents a wide range of pharmacological actions. This study evaluated the effect of Guarana (Paullinia cupana) extract, pure and dry Guarana (PC-18) extract and magnesium chloride (MgCl2) in mice of the Balb/c strain inoculated with the Ehrlich tumor regarding gene expression of inflammatory markers transforming growth factor-β1 and tumor necrosis factor alpha and oxidative stress (OS) and fatigue, superoxide dismutase, catalase, and glutathione peroxidase 4 and analyzed myelotoxicity and hepatotoxicity. After euthanasia, blood was collected to analyze the complete blood count and measured the levels of liver enzymes (alanine aminotransferase and aspartate aminotransferase). Hepatoprotective actions of the crude extract of P. cupana and PC-18 extract were noticed. The PC-18 and MgCl2 group showed the best result regarding animal welfare. There were no associations between compounds and gene expression regarding fatigue and OS. PC-18 reduced the tumor and may have an antitumor action. The crude extract of Guarana presented hepatoprotective action.

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