The first human case of Clinostomum complantatum(Trematoda: Clinostomidae) infection in Korea

Dong-Il CHUNG(정동일),Chu-Hwan MOON(문주환),Hyun-Hee KONG(공현희),Dong-Wik CHOI(최동익),Dong-Kyu LIM(임동규) 대한기생충학열대의학회 1995 The Korean Journal of Parasitology Vol.33 No.3

Demonstration of the second intermediate hosts of Clinostomum complanatum in Korea

Dong-Il CHUNG(정동일),Hyun-Hee KONG(공현희),Chu-Hwan MOON(문주환) 대한기생충학열대의학회 1995 The Korean Journal of Parasitology Vol.33 No.4

The first human case of Diplogonoporus balaenopterae (Cestoda: Diphyllobothriidae) infection in Korea

Dong-Il CHUNG(정동일),Hyun-Hee KONG(공현희),Chu-Hwan MOON(문주환),Dong-Wik CHOI(최동익),Tae-Hyung KIM(김태형),Dong-Wook LEE(이동욱),Jung-Ja PARK(박정자) 대한기생충학열대의학회 1995 The Korean Journal of Parasitology Vol.33 No.3

慶北 漆谷郡 河濱池에서 採集한 淡水魚의 吸蟲類 被囊幼蟲의 奇生狀

林宗燮,文柱煥,鄭東一,崔東翊 慶北大學校 醫科大學 1991 慶北醫大誌 Vol.32 No.3

1990年 4月부터 同年 9月까지 慶北 漆谷郡 河濱池에서 淡水魚를 投綱, 낚시, 사발모지等으로 採集하여 吸蟲類 被囊幼蟲의 魚種別 寄生狀을 調査하였다. 河濱池에서 採集된 5種의 淡水魚에서 5種의 吸蟲類 被囊幼蟲을 同定하였던 바 몰개(Gnathopogon atromaculatus)에서는 5種의 吸蟲類 被囊幼蟲이, 참붕어(Pseudorasbora parva)에서는 3種의 被囊幼蟲이 檢出되었고, 강준치(Erythroculter erythropterus)와 납지리(Paracheilognathus rhombea)에서는 各各 1種의 被囊幼蟲이 檢出되었다. 붕어(Carassius carassius)에서는 吸蟲類 被囊幼蟲이 전혀 檢出되지 않았다. 肝吸蟲 被囊幼蟲의 寄生率과 寄生程度는 몰개에서 各各 58.9% 및 2.0個로 가장 높았으며, Metagonimus屬 被囊幼蟲은 몰개에서만 그 率 3.6%, 그 程度 1.0個로 檢出되었다. 그외의 3種의 吸蟲類 被囊幼蟲, Cyathocotyle orientalis, Metacercaria hasegawai, Pseudexorchis major는 各各 7.4個, 1.0個, 1.0個의 寄生程度를 나타내었다. 이번 調査로 慶北 漆谷郡 河濱池는 吸蟲類의 輕微한 浸潤池임과 河濱池에서 醫學的으로 重要한 淡水魚 宿主는 몰개였다. From April to September, 1990, fresh-water fish collected in Habin pond, Chilgok country, Kyungpook Province, were examined for the determination of the prevalence and intensity of infestation with digenetic larval trematodes. From 5 species of fresh-water fish collected, 5 species of metacercariae were identified. Gnathopogon atromaculatus, the korean shiner, was found to be infested by 5 species of the metacercariae whereas Pseudorasbora parva, the southern top-mouthed minnow, by 4 kinds. Erythroculter erythropterus, the white fish, and Paracheilognathus rhombea, the flat bitterling, were infested with 3 kinds of metacercariae. However, no digenetic larval trematode was demonstrated from Carassius carassius, the crusian carp. The rate and intensity of infestation with Clonorchis sinensis was the highest in G. atromaculatus, 58.9% and 3.4 cysts per gram of flesh, respectively. Metacercaria of Metagonimus species was detected from G. atromaculatus alone. The intensity of fish with the remaining 3 kinds of metacercariae, Cyathocotyle orientalis, Metacercaria hasegawai, and Metorchis orientalis, 7.4, 1.0 and 1.0 cyst per gram of the flesh, respectively. In the light of these results, it seemed likely that Habin pond might be enzootic focus of digenetic larval trematodes and G. atromaculatus might be a fish host of the greatest medical importance.

慶北 永川郡 大勝池에서 採集한 淡水魚의 吸蟲類 肝吸蟲 被囊幼蟲 奇生狀의 變動

李鎭植,文柱煥,鄭東一,崔東翊 慶北大學校 醫科大學 1991 慶北醫大誌 Vol.32 No.3

1990年 4月부터 同年 9月까지 慶北 永川郡 大勝池(사근다리못)에서 採集되는 各種 淡水魚에서 肝吸蟲 被囊幼蟲의 寄生狀을 調査하여 1980年 同貯水池에서 調査한 成績과 比較한 바 採集魚種과 寄生狀의 差異를 觀察하였으므로 報告하는 바이다. 1980년 大勝池에서 採集된 魚種은 붕어(Carassius carassius), 강준치(Erythroculter erythropterus), 버들치(Moroco oxycepharus), 납지리(Paracheilognathus rhombea), 참붕어(Pseudorasbora parva), 참중고기(Sarcocheilichthys sinensis)의 6種이었으나 1990年에는 붕어, 파랑볼우럭(Lepomis macrorchirus), 참붕어의 3種만이 採集되었다. 1980年에 採集한 淡氷魚에서의 肝吸蟲 被囊幼蟲의 寄生率과 寄生程度는 강준치 25.0%, 5.4개, 납지리 13.0%, 0.5개, 참붕어 71.1%, 24.4개, 참중고기 46.7%, 5.8개였고, 1990年에는 참붕어에서만 25.0%, 7.7개로 檢出되었다. 以上의 成績으로 미루어 보아 大勝池는 지난 10年間 生態界가 變化하였고 그간에 移植된 파랑볼우럭이 生態界變化에 多少의 影響을 미친 것으로 생각된다. From April to September, 1990, fresh-water fish were collected in Daesong pond and examined for the presence of Clonorchis sinensis metacercaria in order to compare the infestation with C. sinensis metacercaria with that in 1980. Six species of fresh-water fish, Carassius carassius, the crusian carp, Erythroculter erythropterus, the white fish, Moroco oxycepharus, the fat minnow, Paracheilognathus rhombea, the flat bitterling, Pseudorasbora parva, the southern top-mouthed minnow, and Sarcocheilichthys sinensis, the oily shiner, had been collected in the pond in 1980. In 1990, however, only 3 species of fresh-water fish, C. carassius, P. parva and Lepomis macrorchirus, the blue gill, were collected. The rates and intensity of infestation with C. sinensis had been 71.1% and 24.4 cysts per gram of flesh in P. parva, followed by 46.7% and 5.8 in S. sinensis, 25.0% and 5.4 in E. erythropterus, 13.0% and 0.5 in P. rhombea in 1980. The metacercaria were demonstrated from P. parva alone, with the rate 27.3% and the intensity 7.7, in 1990. In the light of these results, it is likely that L. macrorchirus caused the ecosystem of the pond to be changed.

Efeect of Necropsy Days on Number of Clonorchis sinensis and Plaque Forming Cells after Challenge in Hamsters

Moon, Han Ku,Moon, Chu Hwan,Kong, Hyun Hee,Choi, Dong Wik 慶北大學校 醫科大學 1994 慶北醫大誌 Vol.35 No.2

Challenge 感染된 햄스터에서 屠殺日이 肝吸蟲과 PFC의 數에 미치는 影響을 決定하기 위해 肝吸蟲 成蟲의 代謝産物과 Freund's incomplete adjuvant로 感作된 golden 햄스터의 腹腔渗出細胞와 血淸을 recipient 햄스터의 腹腔內에 注入하였다. 第1次 感作한지 7日 後에 肝吸蟲 被囊幼蟲 20個씩 經口感染시킨 다음, 50日, 70日, 90日 그리고 110日에 各各 屠殺하였다. Challenge感染 後, EpG는 對照群에서 感作群보다 하루 일찍 나타났고, 對照群과 感作群 모두 漸次로 增加되다가 40日에서 65日사이에 最高値를 나타낸 후 漸次 減少하였다. 그러나, 感作群 사이에 EpG의 有意的 差는 認定할 수가 없었다. 蟲體回收率은 感作群과 對照群 사이에 有意的 差를 認定할 수 있었다. 蟲體回收率은 Challenge感染후, 약 70日 前後에 減少되었다. Plaque 形成細胞는 感作後 50日과 70日의 感作群에서 檢出되었으나, 그 以後의 感作群 및 對照群에서는 檢出되지 않았다. 以上의 成績으로 미루어 보아 golden 햄스터의 適切한 屠殺日은 Challenge感染 後 50日에서 70日이 適當함을 알 수 있었다. OBJECTIVE, MATERIALS AND METHODS: To determine the appropriate necropsy day in the passive transfer of immunity against Clonorchis sinensis in golden hamsters, the donor hamsters were sensitized with two injections of the admixture of the metabolic products of C. sinensis and Freund's incomplete adjuvant into footpads at 2 weeks' intervals. The peritoneal cells and sera of sensitized and control donor hamsters were injected intraperitoneally to the recipient hamsters, seven days after primary sensitiza-tion. Four groups of the recipient and control hamsters were challenged with 20 metacercariae of C. sinensis, and killed on the 50th, 70th, 90th and 110th day after challenge infection. RESULTS: The eggs of control hamsters appeared one day earlier than those of the recipient groups, and the eggs per gram of feces in both recipient and control groups increased stepwise and continued at high levels from the 40th to 65th day and then decreased irregularily after challenge infection. There was no significant difference in EpG of feces between the sensitized recipient and control groups, but there was significant difference in the worm burden of C. sinensis between the recipient and control groups. The recovery number of the fluke in the recipient and control hamsters decreased about the 70th day after challenge infection. CONCLUSIONS: These results indicate in part that the appropriate necropsy day of golden hamsters would be estimated around the 50th day after challenge infection of C. sinensis.

Infestation of Clonorchis sinensis Metacercaria from Fresh-water Fish in Pond Kechon

KIM, Byung Chul,MOON, Chu Hwan,KONG, Hyun Hee,CHOI, Dong Wik 慶北大學校 醫科大學 1993 慶北醫大誌 Vol.34 No.1

1991年 5月부터 同年 9月까지 慶北 義城郡 開天池에서 淡水魚를 投網, 사발모지 等으로 採集하여 魚種別 肝吸蟲 被囊幼蟲의 寄生狀을 調査하였다. 開天池에서 採集된 9種의 淡水魚 中 2種의 淡水魚에서 肝吸蟲 被囊幼蟲을 同定하였다. 肝吸蟲 被囊幼蟲의 寄生率은 참붕어(Pseudorasbora parva)에서 48.0%로 몰개(Gnathopogon atromaculatus)의 25.0%보다 높게 나타났다. Cyathocotyle orientalis 被囊幼蟲은 참붕어에서만 42.0%로 나타났다. 寄生程度는 몰개에서 9.5個, 참붕어에서 8.7個 였으며, C. orientalis 被囊幼蟲은 참붕어에서 13.5個로 나타났다. 以上의 成績으로 미루어 보아 慶北 義城郡 開天池는 肝吸蟲의 輕微한 浸潤地였다. In order to determine the rate and intensity of infestation with the metacercaria of Clonorchis sinensis, fresh-water fish were collected in pond Kechon. They were examined for the presence of digenetic larval trematodes in the flesh. Nine kinds of fish were collected in the pond. Of these, the metacercaria of C. sinensis was found in 2 kinds of fish, Gnathopogon atromaculatus and Pseudorasbora parva. The cyst of Cyathocotyle orientalis was found in one kind of fish, P. parva. The infestation rate for the metacercaria of C. sinensis in P. parva was higher than that in G. atromoculatus. The rate of C. orientalis in P. parva was moderate, 42.0%. The intensity of infestation with the metacercariae of G sinensis and C orientalis in the fish was low ranging from 8.7 to 13.5 cysts. It was found that pond Kechon is a weak endemic focus of C. sinensis.

Changing Infestation of Clonorchis sinensis Metacercaia from Fresh-water Fish in Lake Upo

SHIN, Ji Yeol,MOON, Chu Hwan,KONG, Hyun Hee,CHOI, Dong Wik 慶北大學校 醫科大學 1988 慶北醫大誌 Vol.34 No.1

1991年 5月부터 同年 9月까지 慶南 昌寧郡 牛浦에서 淡水魚를 投網, 사발모지 等으로 採集하여 魚種別 肝吸蟲 被囊幼蟲의 寄生狀을 調査하였다. 牛浦에서 採集된 10種의 淡水魚 中 4種의 淡水魚에서 肝吸蟲 被囊幼蟲을 同定하였다. 肝吸蟲 被囊幼蟲의 寄生率은 참붕어(Pseudorasbora parva)에서 85.7%로 가장 높았으며 몰개(Gnathopogon atromaculatus)79.2%, 모래주사(Microphysogobio koreensis) 22.6%의 順으로 나타났다. 寄生程度는 큰납지리에서 58.4個로 가장 높았고 참붕어는 49.3個 였으며, 몰개 및 모래주사는 各各 20.4個와 5.1個로 多少 낮았다. 以上의 成績으로 미루어 보아 慶南 昌率郡 牛浦는 肝吸蟲의 甚한 浸潤地였다. From May to September in 1991, ten kinds of fresh-water fish were collected in lake Upo, Changyong county, Kyungnam Province (Kyung Sang Nam Do), Korea. They were examined for the" ""presence of the metacercaria of Clonorchis sinensis. Four species of fish harbored the cyst, the most frequently infested fish was Pseudorasbora parva 85.7%, followed by Gnathopogon atromaculatus 79.2% and Microphysogobio koreensis 57.1%, and the least frequently infested was Acanthorhodeus taenianalis 22.6%. In intensity of infestation with the metacercaria of C. sinensis, A. taenianalis was the most heavily infested with an average of 58.4 cysts per gram of flesh, followed by P. parva 49.3 cysts. G. atromaculatus and M. koreensis were lightly infested. The results indicate that lake Upo is an heavy endemic focus of C. sinensis.

Infestation of Digenetic Trematode Cercariae from Semisulcospira Snails in Stream Odong

SUH, Chang Sik,MOON, Chu Hwan,KONG, Hyun Hee,CHOI, Dong Wik 慶北大學校 醫科大學 1993 慶北醫大誌 Vol.34 No.1

1991年 6月부터 同年 10月까지 慶北 達城郡 梧洞에서 다슬기를 採集하여 吸蟲類 有尾幼蟲의 寄生狀을 調査하였다. 다슬기에서의 有尾幼蟲의 寄生率은 26%였으며, 3種의 吸蟲類 有尾幼蟲 즉, Centrocestus armatus(Tanabe, 1922) Yamaguti, 1933, Metagonimus species 및 Notocolylus magniovatus, Yamaguti, 1934를 찾아볼 수 있었다. 이 가운데서 Metagonimus species 有尾幼蟲은 가장 많이 遊出되었고, 그 다음은 C. armatus, N. magniovatus의 順位로 遊出되었다. 이번 調査 結果 3種의 吸蟲類 有尾幼蟲의 寄生率은 1980年 보다 多少 增加되었으나, 肺吸蟲 有尾幼蟲은 發見할 수 없었다. 따라서 過去, 肺吸蟲 浸潤地였던 梧洞은 肺吸蟲의 浸潤地가 아님을 알았다. From June to October in 1991, Semisulcospira snails collected in the stream Odong, in Kachang Myon, Dalsong county, Kyungpook Province were examined for the presence of cercariae of digenetic trematodes. The infestation rate for cercariae in 2,437 snails was 26%. Three kinds of cercariae, Centrocestus armatus(Tanabe, 1922), Yamaguti, 1933, Metagonimus species, and Notocotylus magniovatus, Yamaguti, 1934, were found. However, no Paragonimus westermani cercariae was found. The most frequently found cercariae was Metagonimus species 23.5%, followed by C. armalus 2.3%, and the least frequently was N. magniovatus 0.2%. The low density of Semisulcospira snails and no P. westermani cercaria indicate that the stream Odong is no longer an endemic focus of P. westermani.

Infestation of Clonorchis sinensis Metacercaria from Fresh-water Fish in River Wichon

KIM, Hyo Kyung,MOON, Chu Hwan,KONG, Hyun Hee,CHOI, Dong Wik 慶北大學校 醫科大學 1993 慶北醫大誌 Vol.34 No.1

1991年 5月부터 同年 9月까지 慶北 義城郡 渭川에서 淡水魚를 投網, 사발모지 等으로 採集하여 魚種別 肝吸蟲 被囊幼蟲의 寄生狀을 調査하였다. 渭川에서 採集된 14種의 淡水魚 中 6種의 淡水魚에서 肝吸蟲 被囊幼蟲을 同定하였다. 肝吸蟲 被囊幼蟲의 寄生率은 누치(Hemibarbus labeo) 81.1%, 물개(Gnathopogon atromaculatus) 73.3%, 참붕어(Pseudorasbora parva) 66.7%, 큰납지리(Acanthorhodeus taenianalis) 38.9%의 順으로 나타났다. 寄生程度는 참붕어에서 63.0個로 가장 높았고 물개와 돌고기는 各各 10.3個와 16.7個였으며 큰납지리와 누치는 10個 未滿으로 多少 낮게 나타났다. 以上의 成績으로 미루어 보아 慶北 義城郡 渭川은 肝吸蟲의 甚한 浸潤地였다. Fresh-water fish collected in the river Wichon, in Kyungpook Province, Korea were examined for the presence of the metacercaria of Clonorchis sinensis from May to September in 1991. A total of fourteen species of fish were collected, six of which were infested with the metacercaria of C. sinensis. The infestation rates for the cyst were higher in Hemibarbus labeo 100%, Microphysogobio koreensis 81.1% and Pungtungia herzi 100% than Acanthorhodeus taenianalis 38.9%, Gnathopogm atromaculatus 73.7% and Pseudorasbora parva 66.7%. The intensity of infestation with the cyst in the six species of fish except for P. parva was low. The average number of cysts per gram of flesh varied from 2.0 to 16.7. While it was high, 63.0 in P. parva. The results indicate that six kinds of fish collected in the river Wichon are a source of infection of human clonorchiasis.