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Kim, Choon-Mee,Kim, Sam-Cheol,Shin, Sung-Heui The Korean Society for Microbiology 2011 Journal of Bacteriology and Virology Vol.41 No.2
Vibrio vulnificus produces Hemolysin/cytolysin (VvhA), which is one of the most potent exotoxins capable of killing mice at submicrogram levels. However, V. vulnificus growth and vvhA expression are severely repressed and extracellular VvhA produced at low levels is easily inactivated in human body fluids. This study was conducted to obtain additional unequivocal evidence of the enigmatic characteristic of VvhA. V. vulnificus growth was stimulated, vvhA expression was de-repressed, and extracellular VvhA production was increased in cirrhotic ascites, a human ex vivo experimental system, by a mutation of fur encoding ferric uptake regulator, which acts as a transcriptional repressor. However, regardless of the presence or absence of the for mutation, extracellular VvhA activity was not detected in cirrhotic ascites. These results indicate that VvhA is easily inactivated even when vvhA expression and extracellular VvhA production are maintained at high levels in cirrhotic ascites.
Kim, Choon-Mee,Kim, Sam Cheol,Shin, Sung-Heui 朝鮮大學校 附設 醫學硏究所 2009 The Medical Journal of Chosun University Vol.34 No.2
In Vibrio vulnificus, the production of a metalloprotease VvpE is known to be positively regulated by cyclic AMP-receptor protein (CRP), a well-known global regulator. Recently, we found that both 35 and 45 kDa-VvpE, the authentic products of vvpE, are produced within bacterial cells and secreted with the 35 kDa-VvpE being the major secreted form, but the related mechanism remains unknown. In this study, we attempted to determine whether the production profile of VvpE can be affected by the change of CRP. In lacZ-fused transcription reporter assays, crp transcription was increased with V. vulnificus growth and was contemporary with vvpE transcription. The production of VvpE was decreased and delayed along with the down-regulation of vvpE transcription by a null mutation of crp, and recovered by an in trans complementation of crp. However, the production profile of VvpE was not affected by the crp mutation or complementation. These results indicate that CRP controls VvpE production by regulating only vvpE transcription, not affecting the production profile of VvpE.