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수종 구취제거법이 구강내 휘발성 메틸머캅탄 감소에 미치는 영향
이안희,기우천 전남대학교 치과대학 1992 전남치대논문집 Vol.4 No.1
In order to evaluate the effectiveness of tooth brushing mouth gargling and gum chewing in reducing halitosis, 84 individuals ranging in age from 22 to 28 years old were examined. These individuals had no gross oral abnormalities, other than mild gingival inflammation, dental caries, nasopharyngel disorder, or systemic diseases that were associated with halitosis. They were divided into a tooth brushing group, a mouth gargling group, a gum chewing group and a control group that did not use any halitosis removing method. Each of the groups included 21 persons. B.B. Cheker (Tokuyama Soda Co., LTD., Japan) was used to measure the concentrations of intraoral volatile methyl mercaptan of each group. The concentrations of intraoral volatile methyl mercaptan were measured before and after lunch, and after removing halitosis by tooth brushing, mouth gargling and gum chewing. The obtained results were as follows ; 1. The average concentration of intraoral volatile methyl mercaptan before lunch was 1.79ppm and after lunch it was 2.02ppm, an incerase of 12.9%. 2. In the tooth brushing group th average concentration of intraoral volatile methyl mercaptan was 0.61ppm, in the mouth gargling group it was 1.15ppm, in the gum chewing group it was 1.64ppm and in the control group it was 1.92ppm. It decreased 69.5% in the tooth brushing group, 43.8% in the mouth gargling group, 18.4% in the gum chewing group and 5.4% in the control group(P<0.05). 3. There were significant differences between the tooth brushing and control group, tooth brushing and gum chewing group and between mouth gargling and control group in concentrations of intraoral volatile methyl mercaptan after using the halitosis removing methods(P<0.05). According to the above results, tooth brushing and mouth gargling are effective ways to reduce halitosis.
Streptomyces griseus HUT 6037 균주의 키토산분해효소에 의한 키토산 가수분해물 생산 연구
이천우,손정우,김광 동아대학교 생산기술연구소 2000 生産技術硏究所硏究論文集 Vol.5 No.2
To investigate the enzyme activity according to substrate, we used different deacetylated chitosan and CM_-chitosan as a carbon source. We found that this strain accumulated chitosanase in the culture medium containing chitosanaceous substrates instead of chitinaceous substrates. The highest chitosanaceous activity was presented at 4 days cultivation with 99% deacetylated chitosan. The 53% deacetylated chitosan was found that it can produce chitinase as well as chitosanes. It was defined as a soluble chitosan. The specific activities of chitinase and chitosanase were 0.89 and 1.33U/mg protein at 3 and 5 days, respectively. From studies of the enzymatic digestibility of various dgree of deacetylated chitosan and CM_-chitosan, the following results were obtained : chitoanase 99% deacetylated chitosan was suitable for producing the highest specific activity of chotosanase. On the other hand, CM_-chitosan was suitable for producing chitooligosaccharides of (GlcN)_1, (GlcN)_6.
이천우,최종정,김광 東亞大學校附設遺傳工學硏究所 1996 遺傳工學硏究 Vol.- No.3
고탈아세틸화 키토산의 제조에서 중간 생성물의 증류수 세척에 의해서 알칼리 처리시킴으로서 효과적인 탈아세틸화 키토산의 제조가 가능하였고, CM-Chitin은 수용액 상태에서 독특한 고분자 전해질 거동을 나타내며, 점도곡선은 높은 NaCl 농도에서 CM-Chitin 농도에 비례함을 보였다. 그리고, 반응시간에 따른 Chitin의 탈아세틸화는 반응시간에 비례함을 알 수 있었다. Chitin derivatives are difficult to prepare due to the limited alkali-swelling properties of chitin. This communication reports the preparation of a carboxymethylchitin whose properties are sufficiently defined and reproducible to allow the polymer to be used as a potential lysozyme substrate. The carboxymethylchitin whose properties by a new procedure which is more rapid than earlier methods. In the present study, CM-chitin was prepared successfully by simple procedures involving freezing and the addition of a detergent such as sodium dodecyl sulfate(SDS). The solution properties of the water soluble chitin derivatives were studied first. Infrared absorption spectroscopy was employed to investigate the initial alkylation sites on the n-acetylglucosamine residue in chitin molecules.