Grem1 accelerates nucleus pulposus cell apoptosis and intervertebral disc degeneration by inhibiting TGF-β-mediated Smad2/3 phosphorylation

Chen Shunlun,Lei Linchuan,Li Zemin,Chen Fan,Huang Yuming,Jiang Guowei,Guo Xingyu,Zhao Zhuoyang,Liu Hui,Wang Hua,Liu Caijun,Zheng Zhaomin,Wang Jianru 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Intervertebral disc degeneration (IVDD) is a main cause of low back pain, and inflammatory factors play key roles in its pathogenesis. Gremlin-1 (Grem1) was reported to induce an inflammatory response in other fields. This study aimed to investigate the mechanisms of Grem1 in the degenerative process of intervertebral discs. Dysregulated genes were determined by analyzing microarray profiles. The expression of Grem1 in 17 human disc samples (male:female = 9:8) and rat models (n = 5 each group) was measured by western blotting (WB), real-time quantitative PCR (RT-qPCR), and immunohistochemistry (IHC). The regulatory effects of Grem1 on apoptosis were examined using siRNAs, flow cytometry, immunofluorescence (IF), and WB. The therapeutic effect was evaluated by locally injecting specific Grem1 siRNA into IVDD rats. The expression of Grem1 was significantly increased in human degenerative intervertebral discs; furthermore, the expression of Grem1 positively correlated with the level of intervertebral disc degeneration. Grem1 was significantly overexpressed in tumor necrosis factor (TNF)-α-induced degenerative NP cells. Apoptosis in degenerative NP cells transfected with siRNA targeting Grem1 was significantly lower than that in the control group. Specific Grem1 siRNA markedly repressed the development of IVDD in surgery-induced IVDD rats. These results indicated that the expression of Grem1 was positively correlated with the severity of intervertebral disc degeneration, and Grem1 siRNA could inhibit Grem1-induced apoptosis and extracellular matrix alterations by mediating the TGF-β/Smad signaling pathway. This study may provide a therapeutic strategy for alleviating inflammation-induced apoptosis associated with intervertebral disc degeneration.

Chemical kinetics of bean straw biofuel pyrolysis using maximum volatile release method

Wei Chen,Kalyan Annamalai,Jiafeng Sun,Yuming Chen 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.8

Maximum volatile release (MVR) methods, including single point (MVR-S) and multiple (MVR-M) points, are proposed to extract the kinetics used to predict the fuel bean straw pyrolysis process. The simulation results were compared to those of the distributed activation energy model (DAEM). For the TGA (thermogravimetric analysis) experiments, fuels were heated from ambient temperature to 1,173 K at the heating rates of 10, 20, 40, 60, 80 K min−1, and the corresponding maximum volatile release points were obtained. For the MVR-S method, both the activation energy (E) and pre-exponential factor (B) increased with increasing heating rates. For the pyrolysis weight loss process, the DAEM showed best agreement with the experimental data, followed by the MVR-M, and then the MVR-S method. However, for weight loss rate prediction, MVR-S method had the best match with the experimental data, whereas the DAEM and MVR-M method generated more errors.

Fuzzy Synthetic Evaluation of Relay Protection based on Variable Weight Value

Renjie Song,Yuming Chen 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.11

To improve the accuracy of the state evaluation is the most important for the relay protection state maintenance. Therefore, a fuzzy synthetic evaluation of relay protection based on variable weight value is proposed in this paper. Pick up the major factor of the device and secondary circuit of relay protection to establish a reasonable index system of state evaluation. Then according to the variable weight comprehensive concept of factor space theory, we introduce the balance function to adjust the weight coefficient of each factor. Final, get the membership function in triangle trapezoid distribution to show the condition information, and select synthetic operator, then an evaluation system of relay protection with objectively and accurately is proposed. Running examples show that after dealing with the variable weight, some factors weight value become larger or smaller, so running state of evaluate factor may be changed, make it close to the real running state.

New Two-Weight Imbedding Inequalities for A-Harmonic Tensors

GAO, HONGYA,CHEN, YANMIN,CHU, YUMING 대한수학회 2007 Kyungpook mathematical journal Vol.47 No.1

In this paper, we first define a new kind of two-weight-A_(r)^(λ_(3))(λ_(1), λ_(2), Ω)-weight, and then prove the imbedding inequalities for A-harmonic tensors. These results can be used to study the weighted norms of the homotopy operator T from the Banach space L^(P)(D, ∧^(l)) to the Sobolev space W^(1, p)(D, ∧^(l-l), l= 1, 2, · · , n, and to establish the basic weighted L^(P)-estimates for A-harmonic tensors.

Containment Control of Heterogeneous Liner Singular Multi-agent Systems by Distributed Event-triggered Control

Jie Gao,Yuming Chen,Fei Yun 제어·로봇·시스템학회 2024 International Journal of Control, Automation, and Vol.22 No.3

This paper investigates the containment control of heterogeneous linear singular multi-agent systems(MASs) by distributed event/self-triggered control strategy. Firstly, a novel distributed observer is designed to estimate the convex combination of the leaders’ states. Then, a state-feedback controller and an output-feedbackcontroller based on the realtime updating strategy are proposed to obtain the leader-following consensus of the linersingular MASs. Next, in order to reduce the number of the information updating, a novel distributed event/selftriggered controller is designed which only uses the sampled-data information between neighboring agents at theevent-triggered instants. Furthermore, it is proved that the inter-event time is lower bounded by a positive constant and then the Zeno behavior is excluded. Finally, an example is given to verify the effectiveness of the control strategy.

Genetic variation of Vp1 in Sichuan wheat accessions and its association with pre-harvest sprouting response

Jirui Wang,Yaxi Liu,Yan Wang,Zhenhong Chen,Shuai Dai,Wenguang Cao,George Fedak,Xiujing Lan,Yuming Wei,Dengcai Liu,Youliang Zheng 한국유전학회 2011 Genes & Genomics Vol.33 No.2

Pre-harvest sprouting (PHS) in bread wheat is a major abiotic constraint reducing yield and influencing the production of high quality grain. In China both spring and winter wheat regions are affected by PHS. Sichuan lies in southwest China,where the most of rainfall occurs during April to September when wheat is harvested. The present investigation was conducted to identify the allelic variability of Vp1, a gene that plays a role in maintenance and induction of dormancy,among Sichuan landraces and recent cultivars with different dormancy levels and to find potential sources of PHS resistance for breeding. Sichuan landrace and cultivar wheat accessions had a wide range of dormancy levels. The average germination index (GI) of Sichuan landrace accessions was 0.232, whereas at 0.674 it was much higher for cultivars. The different dormancy levels between landraces and cultivars indicated that pre-harvest sprouting resistance might have been neglected in recent Sichuan wheat breeding programs. The average GI of white grained accessions was higher than for red grained accessions. Particular Vp-1B gene fragments were specific in landraces or cultivars and in white or red grained accessions. The results indicated that Vp-1B markers could be used to distinguish cultivars and landraces. Significant relationships between certain Vp-1B allelesand GI of Sichuan wheat accessions were shown by Spearman’s rank correlation analysis.

An Improved Model Predictive Torque Control Strategy of a Shearer Semi-direct Permanent Magnet Synchronous Motor Based on Duty Cycle

Du Wenlong,Li Wei,Lu En,Sheng Lianchao,Chen Yuming,Jiang Song 대한전기학회 2021 Journal of Electrical Engineering & Technology Vol.16 No.5

In order to improve the stability of low-speed and high-torque permanent magnet synchronous motor control in the shearer semi-direct cutting system under large load fl uctuation and strong impact conditions, an improved model predictive torque control strategy based on duty cycle is proposed which can eff ectively reduce the fl ux and torque ripples and improve steadystate control performance. This method combines the separate process of the voltage vector selection and the duty cycle calculation through the predictive model combined with duty cycle. Then, the optimal voltage vector and its duration is selected simultaneously based on minimizing the cost function. In addition, in order to mitigates the computational burden of the proposed method, the voltage vectors in the fi nite control set is pre-selected using hysteresis comparator according to the motor operating status. Furthermore, the duty cycle pulse width modulation is introduced to obtain a fi xed switching frequency of the inverter. The results of simulation experiments confi rm that the proposed strategy can signifi cantly reduce the fl ux and torque ripples and improve the steady state performance while maintaining fast dynamic response.