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Damage and Fracture for Spheroidized Steels during Uniaxial Tensile Testing
Zhou, Fei.,Suh, Chang-Min,Kim, Seock-Sam 경북대학교 트라이볼로지연구소 2001 INTERNATIONAL SYMPOSIUM ON HIGH PERFORMANCE OF TRI Vol.2001 No.-
The mechanical properties, fracture behaviors and micro-mechnism of damage have been studied for three spheroidized steels. It is shown that void formation and growth induced by decohesion at the interface between the ferrite matrix and non-metallic particles or by particle cracking are the main reasons for the failure of these materials during plastic deformation. Experimental results show that the void volume fractions of these materials increase as strain increases, with the increasing rate being larger for the material with more carbide particles. Analytical expressions of strain hardening exponent and fracture strain for plastically damaged materials with second-phase particles are derived, and shown to be in good agreement with experimental data.
REVIEW : Modifiers of TGF-b1 effector function as novel therapeutic targets of pulmonary fibrosis
( Chang Min Lee ),( Jin Wook Park ),( Won Kyung Cho ),( Yang Zhou ),( Bo Ram Han ),( Pyoung Oh Yoon ),( Jei Wook Chae ),( Jack A Elias ),( And Chun Geun Lee ) 대한내과학회 2014 The Korean Journal of Internal Medicine Vol.29 No.3
Pulmonary fibrosis is a fatal progressive disease with no effective therapy. Transforminggrowth factor (TGF)-b1 has long been regarded as a central mediator oftissue fibrosis that involves multiple organs including skin, liver, kidney, and lung. Thus, TGF-b1 and its signaling pathways have been attractive therapeutic targetsfor the development of antifibrotic drugs. However, the essential biological functionsof TGF-b1 in maintaining normal immune and cellular homeostasis significantlylimit the effectiveness of TGF-b1-directed therapeutic approaches. Thus,targeting downstream mediators or signaling molecules of TGF-b1 could be an alternativeapproach that selectively inhibits TGF-b1-stimulated fibrotic tissue responsewhile preserving major physiological function of TGF-b1. Recent studiesfrom our laboratory revealed that TGF-b1 crosstalk with epidermal growth factorreceptor (EGFR) signaling by induction of amphiregulin, a ligand of EGFR, plays acritical role in the development or progression of pulmonary fibrosis. In addition,chitotriosidase, a true chitinase in humans, has been identified to have modulatingcapacity of TGF-b1 signaling as a new biomarker and therapeutic target of scleroderma-associated pulmonary fibrosis. These newly identified modifiers of TGF-b1effector function significantly enhance the effectiveness and flexibility in targetingpulmonary fibrosis in which TGF-b1 plays a significant role.
Improved resolution in single-molecule localization microscopy using QD-PAINT
Chang Yeonho,Kim Do-Hyeon,Zhou Kai,Jeong Min Gyu,Park Soyeon,Kwon Yonghoon,Hong Triet Minh,Noh Jungeun,Ryu Sung Ho 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-
Single-molecule localization microscopy (SMLM) has allowed the observation of various molecular structures in cells beyond the diffraction limit using organic dyes. In principle, the SMLM resolution depends on the precision of photoswitching fluorophore localization, which is inversely correlated with the square root of the number of photons released from the individual fluorophores. Thus, increasing the photon number by using highly bright fluorophores, such as quantum dots (QDs), can theoretically fundamentally overcome the current resolution limit of SMLM. However, the use of QDs in SMLM has been challenging because QDs have no photoswitching property, which is essential for SMLM, and they exhibit nonspecificity and multivalency, which complicate their use in fluorescence imaging. Here, we present a method to utilize QDs in SMLM to surpass the resolution limit of the current SMLM utilizing organic dyes. We confer monovalency, specificity, and photoswitchability on QDs by steric exclusion via passivation and ligand exchange with ptDNA, PEG, and casein as well as by DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) via automatic thermally driven hybridization between target-bound docking and dye-bound complementary imager strands. QDs are made monovalent and photoswitchable to enable SMLM and show substantially better photophysical properties than Cy3, with higher fluorescence intensity and an improved resolution factor. QD-PAINT displays improved spatial resolution with a narrower full width at half maximum (FWHM) than DNA-PAINT with Cy3. In summary, QD-PAINT shows great promise as a next-generation SMLM method for overcoming the limited resolution of the current SMLM.
Fin-Width Effects on Characteristics of InGaAs-Based Independent Double-Gate FinFETs
Chang, Sung-Jae,Zhou, Hong,Gong, Nanbo,Kang, Dong-Min,Lim, Jong-Won,Si, Mengwei,Ye, Peide D.,Ma, T. P. IEEE 2017 IEEE electron device letters Vol.38 No.4
<P>We report the characteristics of InGaAs-based independent double-gate FinFETs with Al2O3/LaAlO3 as gate dielectric. The device can be operated in three different modes ( i. e., single-, double-, and independent double-gate) made possible by the physically separated two sidewall gates. When the device is operated in the double-gatemode, it exhibits better performance in terms of the On/Off current ratio, subthreshold swing, Off current, and channel mobility than in the single-gate mode. In addition, independent double-gate operation makes it possible to modulate channel properties by applying a bias at the opposite gate via gate coupling effects. Our systematicmeasurements reveal that gate control and coupling effects are enhanced with reduced fin width.</P>
Modification and Characterization of Nano-TiO2 for Efficient Fixation on Cotton Fibers
Min Wei,Shugen Wang,Chang Sun,Change Zhou,Jiadong Ni 한국섬유공학회 2018 Fibers and polymers Vol.19 No.11
In this paper, a silane coupling agent, 3-aminopropyltriethoxysilane, was reacted with nano-TiO2 to introduce amino group onto it which was then reacted with trichlorotriazine to obtain a dichlorotriazine functionalized nano-TiO2 for the firm fixation of it on cotton fibers. The reaction process was monitored by the titration of primary and secondary amino groups, and the reaction conditions were optimized with orthogonal method accordingly. The dichlorotriazine functionalized nano-TiO2 was reacted with cotton fabric by the nucleophile substitution reaction to afford nano-TiO2 functionalized cotton fabric, the structure and surface morphology of the nano-TiO2 finished cotton fibers were studied by FT-TR and SEM. In addition, the fixation duration of the nano-TiO2 modified cotton was studied according to AATCC test method 61-2010. The results show that the washing fastness of the nano-TiO2 is excellent.
Jeong, Chang-Bum,Won, Eun-Ji,Kang, Hye-Min,Lee, Min-Chul,Hwang, Dae-Sik,Hwang, Un-Ki,Zhou, Bingsheng,Souissi, Sami,Lee, Su-Jae,Lee, Jae-Seong American Chemical Society 2016 Environmental science & technology Vol.50 No.16
<P>In this study, we evaluated accumulation and adverse effects of ingestion of microplastics in the monogonont rotifer (Brachionus koreanus). The dependence of microplastic toxicity on particle size-was investigated by measuring several in vivo end points and studying the ingestion and egestion using 0.05-, 0.5-, and 6-mu m nonfunctionalized polystyrene microbeads. To identify the defense mechanisms activated in response to microplastic exposure, the activities of several antioxidant-related enzymes and the phosphorylation status of mitogen-activated protein kinases (MAPKs) were determined. Exposure to polystyrene microbeads of all sizes led to significant size dependent effects, including reduced groWth rate, reduced fecundity, decreased lifespan and longer reproduction time. Rotifers exposed to 6-mu m fluorescently labeled microbeads exhibited almost no fluorescence after 24 h, while rotifers exposed to 0.05- and 0.5-mu m fluoresceatly labeled inicrobeads displayed fluorescence until 48 h, suggesting that 6-mu m microbeads are more effectively egested from B. koreanus than 0.05- or 0.5-mu m microbeads. This observation provides a potential explanation for our findings that microbead toxicity was size dependent and smaller microbeads were more toxic. In vitro tests revealed that antioxidant-related enzymes and MAPK signaling pathways were significantly activated in response to microplastic exposure in a size-dependent manner.</P>
Lin, Chang-Ming,Ma, Ji-Min,Zhang, Li,Hao, Zong-Yao,Zhou, Jun,Zhou, Zhen-Yu,Shi, Hao-Qiang,Zhang, Yi-Fei,Shao, En-Ming,Liang, Chao-Zhao Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.10
Transient receptor potential melastain 7 (TRPM7) is a bifunctional protein with dual structure of both ion channel and protein kinase, participating in a wide variety of diseases including cancer. Recent researches have reported the mechanism of TRPM7 in human cancers. However, the correlation between TRPM7 and prostate cancer (PCa) has not been well studied. The objective of this study was to investigate the potential the role of TRPM7 in the apoptosis of PC-3 cells, which is the key cell of advanced metastatic PCa. In this study, we demonstrated the influence and potential function of TRPM7 on the PC-3 cells apoptosis induced by TNF-related apoptosis inducing-ligand (TRAIL). The study also found a novel up-regulated expression of TRPM7 in PC-3 cells after treating with TRAIL. Suppression of TRPM7 by TRPM7 non-specific inhibitors ($Gd^{3+}$ or 2-aminoethoxy diphenylborate (2-APB) ) not only markedly eliminated TRPM7 expression level, but also increased the apoptosis of TRAIL-treated PC-3 cells, which may be regulated by the phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) signaling pathway accompany with up-regulated expression of cleaved Caspase-3, (TRAIL-receptor 1, death receptors 4) DR4, and (TRAIL-receptor 2, death receptors 5) DR5. Taken together, our findings strongly suggested that TRPM7 was involved in the apoptosis of PC-3 cells induced by TRAIL, indicating that TRPM7 may be applied as a therapeutic target for PCa.
Jeong Min Gyu,Zhou Kai,Park Soyeon,An HyeongJeon,Kwon Yonghoon,Chang Yeonho,Kim Do-Hyeon,Ryu Sung Ho 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-
Various repertoires of membrane protein interactions determine cellular responses to diverse environments around cells dynamically in space and time. Current assays, however, have limitations in unraveling these interactions in the physiological states in a living cell due to the lack of capability to probe the transient nature of these interactions on the crowded membrane. Here, we present a simple and robust assay that enables the investigation of transient protein interactions in living cells by using the single-molecule diffusional mobility shift assay (smDIMSA). Utilizing smDIMSA, we uncovered the interaction profile of EGFR with various membrane proteins and demonstrated the promiscuity of these interactions depending on the cancer cell line. The transient interaction profile obtained by smDIMSA will provide critical information to comprehend the crosstalk among various receptors on the plasma membrane.