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- 저자 : Chandra Shakher (검색결과 2 건)
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Role of Arbitrary Intensity Profile Laser Beam in Trapping of RBC for Phase-imaging
Ranjeet Kumar,Vishal Srivastava,Dalip Singh Mehta,Chandra Shakher 한국광학회 2016 Current Optics and Photonics Vol.20 No.1
Red blood cells (RBCs) are customarily adhered to a bio-functionalised substrate to make them stationaryin interferometric phase-imaging modalities. This can make them susceptible to receive alterations in innatemorphology due to their own weight. Optical tweezers (OTs) often driven by Gaussian profile of a laserbeam is an alternative modality to overcome contact-induced perturbation but at the same time a steeplyfocused laser beam might cause photo-damage. In order to address both the photo-damage and substrateadherence induced perturbations, we were motivated to stabilize the RBC in OTs by utilizing a laser beamof ‘arbitrary intensity profile’ generated by a source having cavity imperfections per se. Thus the immobilizedRBC was investigated for phase-imaging with sinusoidal interferograms generated by a compact and robustMichelson interferometer which was designed from a cubic beam splitter having one surface coated withreflective material and another adjacent coplanar surface aligned against a mirror. Reflected interferogramsfrom bilayers membrane of a trapped RBC were recorded and analyzed. Our phase-imaging set-up is limitedto work in reflection configuration only because of the availability of an upright microscope. Due to RBC’smembrane being poorly reflective for visible wavelengths, quantitative information in the signal is weakand therefore, the quality of experimental results is limited in comparison to results obtained in transmissionmode by various holographic techniques reported elsewhere.
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Scientific and Engineering Applications of Full-field Swept-sourceOptical Coherence Tomography
Dalip Singh Mehta,Tulsi Anna,Chandra Shakher 한국광학회 2009 Current Optics and Photonics Vol.13 No.3
We report the development of full-field swept-source optical coherence tomography (SS-OCT) in the wavelength range of 815-870 nm using a unique combination of super-luminescent diode (SLD) as broad-band light source and acousto-optic tunable filter (AOTF) as a frequency-scanning device. Some new applications of full-field SS-OCT in forensic sciences and engineering materials have been demonstrated. Results of simultaneous topography and tomography of latent fingerprints, silicon microelectronic circuits and composite materials are presented. The main advantages of the present system are completely non-mechanical scanning, wide-field, compact and low-cost.
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