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Hybrid Capture 2 Assay Based Evaluation of High-Risk HPV Status in Healthy Women of North-East India
Rai, Avdhesh Kumar,Das, Debabrata,Kataki, Amal Chandra,Barmon, Debabrata,Aggarwal, Dippy,Deka, Pankaj,Shrivastava, Sushruta,Sharma, Jagannath Dev,Sarma, Anupam,Baruah, Upasana,Sharma, Mousumi Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2
Background: High risk HPV (HR-HPV) testing has been recommended as an effective tool along with cytology screening in identification of cervical intraepithelial lesions (CINs) and prevention of their progress towards invasive cervical cancer. The aim of this study was to assess the HR-HPV DNA status by Hybrid Capture 2 (HC2) assay in healthy asymptomatic women of North-East India. Materials and Methods: This study examined cervical cell samples of forty three (n=43) healthy women by HC2 assay. A High Risk HPV DNA kit (Qiagen) was used which can detect 13 high risk HPV types: 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 and 68. Results: The mean relative light units (RLU) for samples was in the range of 141-5, 94, 619. HR-HPV DNA was confirmed in 16% (7/43) of participant women samples. Among demographic and clinical parameters, menstrual irregularity (p=0.039) and infection history (p=0.028) has shown statistically significant differences between the HR-HPV-positive and negative groups. In the HR-HPV positive group, two women were confirmed for CINs after colposcopy and histopathologic examination. Conclusions: We suggest that there may be an association between irregular menstruation and infection history of the urogenital tract with HR-HPV DNA prevalence in North-East Indian asymptomatic women. HC2 assay can be a valuable tool for HR-HPV screening.
Das, Debabrata,Rai, Avdhesh Kumar,Kataki, Amal Chandra,Barmon, Debabrata,Deka, Pankaj,Sharma, Jagannath Dev,Sarma, Anupam,Shrivastava, Sushruta,Bhattacharyya, Mouchumee,Kalita, Apurba Kumar,Sharma, Mo Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2
Background: Persistent infection of one or more of about 15 high-risk human papillomaviruses (HR-HPVs), most commonly HPV types 16/18, has a significant role in cervical cancer initiation and progression. There are limited data available from north-east India about HPV prevalence though this region has high incidence rates of cervical cancer. The aim of this study was to investigate the HPV genotypes prevalent in cervical cancer patients of north-east India. Materials and Methods: We analyzed 107 cervical cancer patient samples. Nested multiplex PCR assays were employed for detection of 13 high risk and 5 low risk HPV types. Results: HPV was confirmed in 105 samples. The presence of 6 'carcinogenic' HPV types, HPV-16 (88%), -18 (15%), -31(4%),-45 (3%), -59 (4%), -58(1%), and one non carcinogenic, HPV-6/11 (6%), was recorded. Among various demographic and clinical factors only tumour stage showed a statistically significant association with HPV type infection (P=0.019). Conclusions: We suggest that the most prevalent genotype is HPV-16 followed by HPV-18 in cervical carcinoma patients of the north-eastern region of India. Advanced tumour stage may be associated with increased possibility of harbouring multiple HPV genotypes.
Assessing Bank Competition in Nepal Using Panzar-Rosse Model
Prem Bahadur BUDHATHOKI,Chandra Kumar RAI,Arjun RAI 한국유통과학회 2020 The Journal of Asian Finance, Economics and Busine Vol.7 No.11
The purpose of this study is to assess the state of competition in Nepalese banking over the period from 2010 to 2019. This study employs panel data and a non-structural Panzar-Rosse model to measure the degree of competition in the Nepalese banking industry. The first reduced-form equation is applied to gauge competition, and the second model is used to test the long-run equilibrium in the banking market. The finding reveals that the Nepalese banking market is equilibrium in the long-run. It implies that the factor prices do not affect ROA in the long-run. The result of the H-statistic shows that the Nepalese banking system is operating under the state of perfect competition and is shifted from monopolistic competition to perfect competition. The reduced-form model reveals that the interest income is positive and significantly affected by factor prices. Similarly, the macroeconomic variable GDP growth is positively related to interest income. On the contrary, the bank’s specific factors risk and the number of bank branches are inversely associated with the regressand. The outcomes of the study may be advantageous to the policymakers, especially to Nepal Rastra Bank to implement monetary policy and M&A policy for the stability and growth of the financial system of Nepal.
Reddy, Rangareddygari Manohar,Suryanarayana, Nagabathula,Rai, Suresh,Sinha, Manoj Kumar,Hansda, Ganga,Ojha, Nand Gopal,Prasad, Bhagwan Chandra Korean Society of Sericultural Science 2010 International Journal of Industrial Entomology Vol.20 No.2
The bivoltine tropical tasar ecoraces of Antheraea mylitta Drury produces cocoons for seed production under seed crop rearing (July-August with $22{\sim}30^{\circ}C$ temperature and 50~70% relative humidity) and for silk production under commercial crop rearing (September-December with $17{\sim}27^{\circ}C$ temperature and 60~80% relative humidity). To consider the impact of hybrid vigour on egg, larva, cocoon and silk related commercial traits, the $F_1$ hybrids made among Daba, Jata and Raily ecoraces were assessed successively for three years. The hybrid vigour in the $F_1$ hybrid of Daba$\times$Jata ($T_7$) was positive for egg fertility (+23.1%), shell weight (+25.6%), silk yield (+79.0%) and filament length (+68.1%), with filament of high denier (11.98d) and reduced larval span (-7.1%). The Jata$\times$Daba ($T_8$) hybrid has shown negative heterosis in egg fertility (-8.0%) compared to the other F1 hybrids, Daba$\times$Raily ($T_9$) and Raily$\times$Daba ($T_{10}$), when they rise simultaneously during the commercial crop season. The better performance of parental ecoraces ($T_4$ to $T_6$) in their commercial traits during commercial crop over parents of seed crop ($T_1$ to $T_3$) and the superior performance of $F_1$ hybrids ($T_7$ to $T_{10}$) over parents of commercial crop ($T_4$ to $T_6$) during commercial crop season indicates the apparent hybrid vigour in tasar $F_1$ hybrids. As the Daba$\times$Jata ($T_7$) and Jata$\times$Daba ($T_8$) $F_1$ hybrids have shown highest hybrid vigour, their rearing during commercial crop can optimize the silk productivity and commercial sustenance of the tasar silk industry.
( Rangareddygari Manohar Reddy ),( Nagabathula Suryanarayana ),( Suresh Rai ),( Manoj Kumar Sinha ),( Ganga Hansda ),( Nand Gopal Ojha ),( Bhagwan Chandra Prasad ) 한국잠사학회 2010 International Journal of Industrial Entomology Vol.20 No.2
The bivoltine tropical tasar ecoraces of Antheraea mylitta Drury produces cocoons for seed production under seed crop rearing (July-August with 22~30˚C temperature and 50~70% relative humidity) and for silk production under commercial crop rearing (September-December with 17~27˚C temperature and 60~80% relative humidity). To consider the impact of hybrid vigour on egg, larva, cocoon and silk related commercial traits, the F1 hybrids made among Daba, Jata and Raily ecoraces were assessed successively for three years. The hybrid vigour in the F1 hybrid of Daba×Jata (T7) was positive for egg fertility (+23.1%), shell weight (+25.6%), silk yield (+79.0%) and filament length (+68.1%), with filament of high denier (11.98d) and reduced larval span (-7.1%). The Jata×Daba (T8) hybrid has shown negative heterosis in egg fertility (-8.0%) compared to the other F1 hybrids, Daba×Raily (T9) and Raily×Daba (T10), when they rise simultaneously during the commercial crop season. The better performance of parental ecoraces (T4 to T6) in their commercial traits during commercial crop over parents of seed crop (T1 to T3) and the superior performance of F1 hybrids (T7 to T10) over parents of commercial crop (T4 to T6) during commercial crop season indicates the apparent hybrid vigour in tasar F1 hybrids. As the Daba×Jata (T7) and Jata×Daba (T8) F1 hybrids have shown highest hybrid vigour, their rearing during commercial crop can optimize the silk productivity and commercial sustenance of the tasar silk industry.