http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
María del Carmen Oliver-Salvador,Elisa Morales-López,Enrique Durán-Páramo,Carlos Orozco-Álvarez,Sergio García-Salas 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.4
Cysteine proteases from Jacaratia mexicana,an endemic Mexican plant, could compete in industrial applications with papain. Currently the only way to obtain these proteases is by extracting them from the wild plant. An alternative source of these enzymes is by J. mexicana suspension culture. In this work, this culture was carried out in airlift, bubble column and stirred tank bioreactors,and the effects of shear rate and microturbulence on cell growth, protein accumulation and proteolytic activity were determined. The shear rates in the stirred tank, bubble column and airlift bioreactors were 274 1/s, 13 1/s and 36 1/s respectively, and microturbulences (symbolized by λ, in units of μm) were 46, 79, and 77 μm, respectively. Protein levels and proteolytic activity were linearly correlated with both shear rate and microturbulence. A higher shear rate and a more intensive microturbulence occurred in the stirred tank, producing higher protein accumulation and higher proteolytic activity compared with those of the other two bioreactor systems. Higher shear rate and microturbulence had an elicitor effect on protease synthesis, because microturbulence in stirred tank bioreactors was lower than the average length of J. mexicana cells. Furthermore, cells in the stirred tank were smaller and thinner than those grown in shake flask, bubble column and airlift bioreactors. In summary, proteases were produced by J. mexicana cell cultures in a stirred tank under conditions of high shear rate and intensive microturbulence, which are similar to those which occur in industrial stirred tanks. These results encourage continuation of the process development for large scale production of these proteases by this technology.
Ilse-Yazmín Arciniega-Carreón,María-Guadalupe Ramírez-Sotelo,Ana C. Ramos-Valdivia,Carlos Edmundo Salas,Arturo Ortega,Carmen Oliver-Salvador 한국원예학회 2020 Horticulture, Environment, and Biotechnology Vol.61 No.6
Ibervillea sonorae (S. Watson) Greene, (huereque) is an endemic shrub whose roots are used in traditional medicine to treatdiabetes. Here, we establish cell cultures of I. sonorae to evaluate the presence of antidiabetic metabolites. Stem and rootssections were incubated on media with diff erent concentrations of growth regulators to induce calli. Using B5 mediumcontaining 25 g L −1 sucrose, 150 mg L −1 ascorbic acid, 6 g L −1 agar–agar, supplemented with 0.5 mg L −1 α-naphthaleneaceticacid, 0.5 mg L −1 benzyladenine and 1 mg L −1 indole-3-acetic acid, 95% calli induction was attained. Flavonoids andphenols in I. sonorae cultured cell suspension were investigated. The methanol extracts exhibited antioxidant activity thatwas attributed to phenolic compounds. These extracts stimulated glucose uptake more effi caciously than I. sonorae plantroots. This study demonstrates that in vitro cultured cells stand as a good option for production of hypoglycemic metabolites.