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Yin, Dongfeng,Chu, Cang,Ding, Xueying,Gao, Jing,Zou, Hao,Gao, Shen The Polymer Society of Korea 2009 Macromolecular Research Vol.17 No.1
In order to enhance the gene delivery efficiency and decrease the cytotoxicity of polyplexes, we synthesized Solutol-g-PEI by conjugating polyethyleneimine (PEI) to Solutol (polyoxyethylene (10) stearate), and evaluated its efficiency as a possible nonviral gene carrier candidate. Structural analysis of synthesized polymer was performed by using $^1H$-NMR. Gel retardation assay, particle sizes and zeta potential measurement confirmed that the new gene carrier formed a compact complex with plasmid DNA. The complexes were smaller than 150 nm, which implicated its potential for intracellular delivery. It showed lower cytotoxicity in three different cell lines (Hela, MCF-7, and HepG2) than PEI 25 kDa. pGL3-lus was used as a reporter gene, and the transfection efficiency was in vitro measured in Hela cells. Solutol-g-PEI showed much higher transfection efficiency than unmodified PEI 25 kDa.
Zhou Jinjin,Hao Xiuzhen,Chu Longgang,Cang Long 한국화학공학회 2023 Korean Journal of Chemical Engineering Vol.40 No.7
Organic pollutant removal can be realized via electrochemical degradation, and anodic oxidation plays an important role in the degradation process. However, the degradation process is hindered by low removal efficiency and reusability. In this study, a PbO2 electrode and a Mn-doped PbO2 (Mn-PbO2) electrode were prepared via electrodeposition. Different from the traditional method of increasing the oxygen evolution potential (OEP) of PbO2 electrode, such as introducing Ce, Bi, and La in PbO2 electrode, introducing Mn in PbO2 electrode promoted the generation of strong oxidant MnO4 under electrolytic conditions to remove organic pollutants. The Mn-PbO2 electrode was used for anthracene (ANT) degradation and the results showed that an ANT removal efficiency of 91.28% was achieved in 120 min under the most suitable operational variables, namely, a Na2SO4 concentration of 0.1 M, initial pH of 7, and current density of 20 mA/cm2. In addition, the ANT degradation mechanism was analyzed. Given that the Mn-PbO2 electrode exhibited better removal efficiency in the wide pH range and remarkable degradation efficiency compared with the PbO2 electrode, the Mn-PbO2 electrode has practical application prospects for organic pollutant degradation.