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Chang, Jun,Jang, Ji Eun,Yu, Jae Rang,Kim, Sol 梨花女子大學校 藥學硏究所 2011 藥學硏究論文集 Vol.- No.21
Respiratory syncytial virus (RSV) is a major cause of severe lower respiratory tract disease in infancy and early childhood. Despite its importance as a pathogen, there is no licensed vaccine against RSV. The fusion (F) protein of RSV is a potentially important target for protective antiviral immune responses. Here, we studied the immune responses elicited by recombinant replication-deficient adenovirus (rAd)-based vaccines expressing the soluble F1 fragment of F protein (amino acids 155-524) in murine model. The expression of secreted F1 fragment by rAd was significantly increased by codon optimization. Strong mucosal IgA response was induced by single intranasal immunization of codon-optimized vaccine, rAd/F1co, but not by rAd/F1wt. A single intranasal immunization with rAd/F1co provided potent protection against subsequent RSV challenge. Interestingly, neither serum Ig nor T-cell response directed to F protein was detected in the rAd/F1co-immune mice, suggesting that protective immunity by rAd/F1co is mainly mediated through mucosal IgA induction. Indeed, co-delivery of cholera toxin B subunit significantly enhanced mucosal IgA responses by the optimized vaccine, which correlates with protective efficacy. Taken together, our data demonstrate that a single intranasal administration of rAd/F1co is sufficient for the protection and represents a promising prophylactic vaccination regimen against RSV infection.
Chang, Jun 이화여자대학교 약학연구소 2008 藥學硏究論文集 Vol.- No.17
In vitro large amplification of tumor-specific cytotoxic T lymphocytes (CTLs) and adoptive transfer of these cells is one of the most promising approaches to treat malignant diseases in which an effective immune response is not achieved by active Immunization. However, generating sufficient numbers of tumor-specific CTLs stimulated with autologous antigen presenting cells (APCs) in vitro is one of the most problematic steps in the adoptive cell transfer (ACT) therapy. To circumvent this problem, we have developed an artificial antigen presenting Complex (aAPCs) using MHC class 1 molecules loaded with a melanoma-specific TRP-2 peptide epitope. Our results show that TRP-2-specific CD8^(+) T cells elicited by immunization with recombinant adenovirus expressing the mini-gene epitope are efficiently stimulated and amplified in vitro to a greater extent by aAPCs than by natural splenic APCU. These aAPC-induced CTLs recognized endogenously pro - cessed antigens present on B16F10 melanoma cells. Efficient stimulation and proliferation of antigen- specific T cells was also confirmed using ovalbumin peptide-loaded aAPCs and OT-I TCR transgenic cells. These results demonstrate that prior in vivo immunization, which increases the precursor frequency, simplifies posterior expansion of tumor- specific CD8_(+) T cells, and aAPCs is superior to autologous APC for in vitro amplification. This ""prime and expand"" regimen can be an alternative method for large amplification of rare tumor-specific CTLs and aAPCs should be a useful tool for ACT immunotherapy.
Intranasal Delivery of Cholera Toxin Induces Th 17- Dominated T-Cell Response to Bystander Antigens
Chang, Jun,Lee, Jee Boong,Jang, Ji Eun,Song, Man Ki 이화여자대학교 약학연구소 2010 藥學硏究論文集 Vol.- No.20
Cholera toxin (CT) is a potent vaccine adjuvant, which promotes mucosal immunity to protein antigen given by nasal route. It has been suggested that a promotes T helper type 2 (Th2) response and suppresses Th1 response. We here report the induction of Th17-dominated responses in mice by intranasal delivery of CT. This dramatic Th17-driving effect of CT, which was dependent on the B subunit, was observed even in Th1 or Th2-favored conditions of respiratory virus infection. These dominating Th17 responses resulted in the significant neutrophil accumulation in the lungs of mice given CT. Both in vitro and in vivo treatment of CT induced strongly augmented IL-6 production, and Th17-driving ability of CT was completely abolished in IL-6 knockout mice, indicating a role of this cytokine in the Th17-dominated T-cell responses by CT. These data demonstrate a novel Th17-driving activity of CT, and help understand the mechanisms of a adjuvanticity to demarcate Thelper responses.
Chang Jun,Jia Fukang,Mumford Kathryn A.,Yang Xiaohong,Xie Zinan 한국자원공학회 2020 Geosystem engineering Vol.23 No.5
This work describes the separation of Ni and Co from a real feed solution in chloride media with high Ni content. The extractant used was P507 (2-ethylhexyl phosphoric acid-2-ethylhexyl ester) in a laboratory scale Impinging Stream–Rotating Packed Bed (IS-RPB) contactor. Key variables including feed solution pH, extractant concentration, saponification degree, high gravity level and total volumetric flow rate of fluids amongst other variables were examined. A McCabe–Thiele diagram of the extraction stage predicted that at a total volumetric flow rate of 90 L/h, feed solution pH of 5, saponified P507 concentration of 25% and high gravity of 83, almost 99% of cobalt, would be extracted within three stages. With regard to the scrubbing results, the co-extracted nickel was scrubbed efficiently from the loaded organic solution using a mixture of 0.02 M CoCl2 and 0.5 M hydrochloric acid solution, The purified Co solution was achieved after stripping the loaded-organic phase with 1 M HCl. The maximum value of the overall volumetric mass transfer coefficient for the IS-RPB extractor was found to be 0.16 s−1 which was superior to that of conventional extractors such as mixer-settler and centrifugal extractors, providing strong confidence for its industrial application.
MHC multimer: A Molecular Toolbox for Immunologists
Chang Jun 한국분자세포생물학회 2021 Molecules and cells Vol.44 No.5
The advent of the major histocompatibility complex (MHC) multimer technology has led to a breakthrough in the quantification and analysis of antigen-specific T cells. In particular, this technology has dramatically advanced the measurement and analysis of CD8 T cells and is being applied more widely. In addition, the scope of application of MHC multimer technology is gradually expanding to other T cells such as CD4 T cells, natural killer T cells, and mucosalassociated invariant T cells. MHC multimer technology acts by complementing the T-cell receptor-MHC/peptide complex affinity, which is relatively low compared to antigen-antibody affinity, through a multivalent interaction. The application of MHC multimer technology has expanded to include various functions such as quantification and analysis of antigen-specific T cells, cell sorting, depletion, stimulation to replace antigen-presenting cells, and single-cell classification through DNA barcodes. This review aims to provide the latest knowledge of MHC multimer technology, which is constantly evolving, broaden understanding of this technology, and promote its widespread use.
Tail behavior of the sums of dependent and heavy-tailed random variables
Changjun Yu,Yuebao Wang,Dongya Cheng 한국통계학회 2015 Journal of the Korean Statistical Society Vol.44 No.1
In this paper, we investigate the tail asymptotic behavior of the partial sums, therandom sums and the weighted sums of heavy-tailed random variables (r.v.s.) undertwo new dependence structures, respectively. The increments are real-valued and havesubexponential∗ distributions, and the dependence structures can contain common linearlynegatively quadrant dependent r.v.s., some positively dependent r.v.s. and some otherr.v.s. The obtained results are used to derive the asymptotic estimation of the finite-timeruin probability for a nonstandard renewal risk model. In addition, some mutual relationsamong these two new dependence structures and some other relevant ones are discussed.