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- 저자 : Buddhi P. Sharma (검색결과 1 건)
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Shambhu P. Dhital,Hak Tae Lim,Buddhi P. Sharma 한국원예학회 2008 Horticulture, Environment, and Biotechnology Vol.49 No.1
In vitro plantlets of potato genotype ‘F9-99’ infected with potato leaf roll virus (PLRV) and potato virus Y (PVY) were used for virus elimination studies using electric current and chemical treatments. Virus status of in vitro plantlets was determined by double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA) and reverse transcription polymerase chain reaction (RT-PCR) before and after treatment. The highest (62.5%) and the lowest (21.9%) regeneration rates were observed from the shoot buds excised after electric treatments with 10 miliampers (mA) for 5 min and 15 mA for 10 min, respectively; also, there were no significant effect on plant growth after electric treatment, except for very high mA for long time. As for virus elimination, current supplied at 10 mA for 5 min resulted in the highest rate of PLRV (46.7%) and PVY (40%) elimination, whereas the lowest virus elimination rate was from the lowest electric current applied for short duration in both of the potato viruses. Study also showed that the plantlets regenerated after electric treatments had higher percentages of virus-free from the shoot buds excision than the nodal cuttings. In the case of electric and chemical treatment combination, the highest rates of PLRV (67.2%) and PVY (62.8%) free plantlets were obtained from shoot buds excised after electrotherapy and cultured on MS medium containing 3% sucrose, 0.7% agar and addition of ribavirin (20 ㎎ㆍℓ?¹) and acetylsalicylic acid (ASA) (10?? M). However, the virus concentration was highly reduced by the addition of ribavirin in the culture medium and the regeneration rates were the highest (72.7%) from the ASA added treatment. Leaf and tuber morphology of plant regenerated from electrotherapy and chemotherapy were similar to those from non-treated control plants. Similar results of virus elimination were obtained under in vitro and in vivo conditions after treatment. Therefore, this study confirmed that electrotherapy (10 mA for 5 min) with the addition of ribavirin (20 ㎎ㆍℓ?¹) and ASA (10?? M) into the cultural medium was the most effective for virus elimination from the virus infected plantlets under in vitro condition and acetylsalicylic acid (ASA).
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