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      • KCI등재

        Local immune cell contributions to fracture healing in aged individuals – A novel role for interleukin 22

        Bucher Christian H.,Berkmann Julia C.,Burkhardt Lisa-Marie,Paschke Carolin,Schlundt Claudia,Lang Annemarie,Wolter Angelique,Damerau Alexandra,Geissler Sven,Volk Hans-Dieter,Duda Georg N.,Schmidt-Bleek 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

        With increasing age, the risk of bone fractures increases while regenerative capacity decreases. This variation in healing potential appears to be linked to adaptive immunity, but the underlying mechanism is still unknown. This study sheds light on immunoaging/inflammaging, which impacts regenerative processes in aging individuals. In an aged preclinical model system, different levels of immunoaging were analyzed to identify key factors that connect immunoaged/inflammaged conditions with bone formation after long bone fracture. Immunological facets, progenitor cells, the microbiome, and confounders were monitored locally at the injury site and systemically in relation to healing outcomes in 12-month-old mice with distinct individual levels of immunoaging. Bone tissue formation during healing was delayed in the immunoaged group and could be associated with significant changes in cytokine levels. A prolonged and amplified pro-inflammatory reaction was caused by upregulated immune cell activation markers, increased chemokine receptor availability and a lack of inhibitory signaling. In immunoaged mice, interleukin-22 was identified as a core cell signaling protein that played a central role in delayed healing. Therapeutic neutralization of IL-22 reversed this specific immunoaging-related disturbed healing. Immunoaging was found to be an influencing factor of decreased regenerative capacity in aged individuals. Furthermore, a novel therapeutic strategy of neutralizing IL-22 may successfully rejuvenate healing in individuals with advanced immune experiences.

      • SCIESCOPUS

        Application of joint time-frequency distribution for estimation of time-varying modal damping ratio

        Bucher, H.,Magluta, C.,Mansur, W.J. Techno-Press 2011 Structural Engineering and Mechanics, An Int'l Jou Vol.37 No.2

        The logarithmic decrement method has been long used to estimate damping ratios in systems with only one modal component such as linear single degree of freedom (SDOF) mechanical systems. This paper presents an application of a methodology that uses joint time-frequency distribution (JTFD) as input, instead of the raw signal, to systems with several vibration modes. A most important feature of the present approach is that it can be applied to a system with time-varying damping ratio. Initially the precision and robustness of the method is determined using a synthetic model with multiple harmonic components, one of them displaying a time-varying damping ratio, subsequently the results obtained from experiments with a reduced model are presented. A comparison is made between the results obtained with this methodology and those using the classical technique of Least Squares Complex Exponential Method (LSCE) in order to highlight the advantages of the former, such as, good precision, robustness and excellent performance in extreme cases, e.g., when very low frequency components and time varying damping ratio are present.

      • KCI등재

        Application of joint time-frequency distribution for estimation of time-varying modal damping ratio

        H. Bucher,C. Magluta,W.J. Mansur 국제구조공학회 2011 Structural Engineering and Mechanics, An Int'l Jou Vol.37 No.2

        The logarithmic decrement method has been long used to estimate damping ratios in systems with only one modal component such as linear single degree of freedom (SDOF) mechanical systems. This paper presents an application of a methodology that uses joint time-frequency distribution (JTFD) as input, instead of the raw signal, to systems with several vibration modes. A most important feature of the present approach is that it can be applied to a system with time-varying damping ratio. Initially the precision and robustness of the method is determined using a synthetic model with multiple harmonic components, one of them displaying a time-varying damping ratio, subsequently the results obtained from experiments with a reduced model are presented. A comparison is made between the results obtained with this methodology and those using the classical technique of Least Squares Complex Exponential Method (LSCE) in order to highlight the advantages of the former, such as, good precision, robustness and excellent performance in extreme cases, e.g., when very low frequency components and time varying damping ratio are present.

      • KCI등재

        Semantische und pragmatische Aspekte der Personen-und Sozialdeixes

        Stefan Bucher 언어과학회 1991 언어과학연구 Vol.8 No.-

        인칭지시는 대화 참여자의 역할, 즉 화자, 청자, 비-대화 참여자를 지시하는 것이며, 따라서 인칭지시에서는 대화 참여자의 역할이 어휘화되고 문법화된다. 그러나 독일어에서 1인칭 복수 "wir"가 여러 명의 화자를 가리키는 경우는 드물고, 대개는 화자가 포함된 다수를 지시한다. 그래서 복수 1인칭 대명사는 1) 화자+화자, 2) 화자+청자, 3) 화자+비-대화 참여자 등과 같은 세가지 기본적 지시 유형을 가진다. 복수 2인칭 대명사 "ihr"는 1) 청자+청자, 2) 청자+비-대화 참여자, 그리고 복수 3인칭 대명사 "sie"는 다수의 비-대화 참여자를 각각 지시한다. 사회적 관계의 지시는 대화 참여자의 사회적 주체성과 대화 참여자 사이의 사회적 관계를 표현하는 언어구조의 한 측면이다. 사회적 관계의 지시에는 상대적인 지시와 절대적인 지시가 구별된다. 절대적인 지시표현은 일정한 화자 또는 청자에게 지속적으로 유지되어 있는 형식으로서, 가령 독일어에서 제왕의 복수 "wir"가 그것이다. 상대적인 지시형식에 있어서는 화자와 피지시자 사이의 상대적 지위 관계 또는 존경 관계를 지시하는 예의 형식인 피지시자-경칭과 그리고 청자에 대한 존경이 그를 지시하지 않고, 대체될 수 있는 동시 접미사를 선택함으로써 문법화 되는 청자-경칭이 있다. 한국어는 청자-경칭과 피지시자-경칭의 혼합형을 갖고 있다. 복수 1인칭 "wir"는 앞서 기술한 지시 가능성으로부터 일탈된 의미로 사용되는 경우가 훨씬 더 많다. "wir"가 가진 의미 다양성을 설명하고자 화용론에서는 의미 핵이 맥락과 상황에 따르는 대화적 함축에 의해 층을 이루고 있다고 주장한다. 맥락과 상황에 좌우되지 않는 관례적 함축은 특수한 경우로서, 가령 독일어에서 단수, 복수-교체가 나타나는 제왕의 복수가 그것이다. 이 경우에서는 자구적 의미로부터 발화 의미가 추론되지 않고, 다만 관례적으로 확정된 사회적 지시행위가 있을 따름이다. 그러나 대화적 함축의 경우는 자구적 의미에서부터 실제적인 발화 의미가 추론되며, 이 때의 추론은 맥락과 상황에 따라 좌우된다. 가령 화학교사가 학생들 앞에서 실험하면서, 간호원이 환자에게 또는 우승한 선수의 아버지가 사용한 "wir"와 결합되어 있는 대화적 함축은 각기 한 사람의 화자, 청자 또는 비-대화 참여자를 지시한다고 추론한다고 할 수 있다.

      • KCI등재
      • INNOVATION STRATEGIES OF INDEPENDENT LUXURY BRANDS

        Jonas Hoffmann,Laurent Lecamp,Carl F. Bucherer 글로벌지식마케팅경영학회 2015 Global Fashion Management Conference Vol.2015 No.06

        The world of luxury is harboring an endangered species: that of the independent companies! In an increasingly-challenging and globalized luxury environment, companies are fighting to escape from the ever-growing clout of luxury “conglomerates” - Swatch Group, LVMH, Kering, and Richemont. As of 2015, these “big four” own more than 100 brands and are maintaining a constant pace of acquisitions , relying on vertical integration to secure supplies (and deprive competitors of them) which has particularly insidious consequences for independents. Most independents are struggling to survive and end up being acquired or going out of business. Interestingly, this same movement towards consolidation is rendering brands more and more uniform, thus creating opportunities for players able to craft unique offerings for niche luxury clientele. Innovation is the path to follow in this challenging journey. Based on a multiple case study analysis of innovative independent luxury companies, we have identified four innovation strategies (Hoffmann & Lecamp, 2015). “Back to the roots” include independent companies innovating in the essence of luxury: extreme quality and extraordinary craftsmanship to create the ultimate sensorial and emotional experience. For companies like Vignes, Thomas Mercer, Mirazur by Mauro Collagreco, Norlha and Brunello Cucinelli, luxury is rooted in a terroir and a sense of purpose permeates this endeavor. “Code breakers” are playing with product and societal codes to culturally innovate and build the icons of today and tomorrow. Examples include Martin Margiela and Miuccia Prada in fashion, Fernando & Humberto Campana in furniture design, and HYT in watchmaking. “Eagle in the aquarium” companies are disrupting the way luxury companies create, deliver and capture value. Globalization and digitization are powerful enablers to reconfigure resources at the levels of funding (e.g. crowdfunding), design (e.g. 3D design), manufacturing (e.g. 3D printing), distribution (e.g. online platforms), marketing and communication (e.g. online social networks). “Game changers” are building breakthrough innovations at the product and business model levels. Comme des Garçons, Etudes Studio, Iris Van Herpen, MB&F, and W Motors are independents taking bold initiatives in a fascinating and inspiring journey. Managerial implications and venues for future research will be highlighted during the conference.

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