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Characterization of Some Potentially Probiotic Lactobacillus Strains of Human Origin
Jelena Novaković Jovanović,Biljana Nikolić,Svetlana Šeatović,Gordana Zavišić,Dragana Mitić-Ćulafić,Branka Vuković-Gačić,Jelena Knežević-Vukčević 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.5
A novel preparation for human use was investigated for probiotic properties of new lactobacilli isolates from oral and fecal samples of children. Identified strains were Lactobacillus plantarum (Lac1, Lac2, Lac6, and Lac7), Lactobacillus casei (Lac3), and Lactobacillus paracasei (Lac4). Isolates were non-hemolytic, produced organic acids, were tolerant to wide ranges of temperature, NaCl, and pH, and were highly resistant to lysozyme, acidity, and bile salts. High survival rates in artificial gastric and intestinal fluids indicated abilities to survive passage through the gastrointestinal tract. Antimicrobial activities were restricted to bacteria, attributed to low pH values. Isolated strains possessed good aggregation abilities, high hydrophobicity values, and moderate abilities to adhere to HCT 116 cells. Substantial probiotic features were identified for all isolates. Lac2, Lac6, and Lac7 were identified as the most advantageous candidates for further study of other probiotic and technological properties.
Shestakova Anna,Osmolovskiy Alexander,Lavrenova Viktoria,Surkova Daria,Nikolić Biljana,Savković Željko 한국미생물·생명공학회 2023 한국미생물·생명공학회지 Vol.51 No.4
Proteolytic enzymes produced by filamentous fungi can degrade various fibrous and globular proteins along with other metabolites that may also find application in biotechnology. In this study, the effect of proteolytic enzymes of 22 Aspergillus strains on various proteins was investigated using protein-containing diagnostic media. Subsequently, a new parameter estimating secreted proteinases specificity towards fibrous or globular proteins without its advanced biochemical research - index of severity of proteolytic action (ISPA) - was suggested. This index determines mycozymes specificity in following manner: its value increases with greater affinity to fibrous proteins, decreases if there is higher affinity to globular proteins. ISPA value was the lowest (0.52) for Aspergillus domesticus, indicating the highest specificity to globular proteins, the highest one (1.26) for A. glaucus, whose proteinases best hydrolyzed fibrous proteins. However, the highest overall proteolytic potential was observed for Aspergillus melleus. The ability to produce acid, alkali and extracellular pigments was evaluated for all isolated strains as well.