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Wu Guoxiu,Li Shengli,Dong Yaoxin,Bi Huangai,Ai Xizhen 한국원예학회 2022 Horticulture, Environment, and Biotechnology Vol.63 No.5
Hydrogen sulfi de (H 2 S) and hydrogen peroxide (H 2 O 2 ) play signifi cant roles as signaling molecules in multiple physiological activities. However, the mechanism and relationship between H 2 S and H 2 O 2 in regulating chilling tolerance remain unclear. This study found that chilling stress induced both endogenous H 2 S and H 2 O 2 systems in cucumber seedlings. Exogenous NaHS (an H 2 S donor) treatment notably strengthened the mRNA abundance of respiratory burst oxidase homolog ( RBOH ), contributing to endogenous H 2 O 2 accumulation. However, H 2 O 2 had little eff ect on mRNA abundances and activities of L-/D-cysteine desulfhydrase (L/DCD), and endogenous H 2 S levels. NaHS and H 2 O 2 increased chilling tolerance in cucumber seedlings, as shown by decreased electrolyte leakage (EL), chilling injury indexes, reactive oxygen species (ROS), and malonaldehyde (MDA) content, and improved activities and expression levels of antioxidant enzymes as well as glutathion (GSH) and ascorbic acid (AsA) contents. Additionally, the photosynthetic rates (Pn), actual photochemical effi ciency (φPSII), maximum photochemical effi ciency (Fv/Fm), and mRNA abundances and activities of Calvin cycle enzymes (Rubisco, SBPase, FBA, and TK) were induced by NaHS and H 2 O 2 . Interestingly, the H 2 S-induced chilling tolerance was suppressed by dimethylthiourea (DMTU, an H 2 O 2 scavenger). The results illustrated that H 2 S repressed the negative eff ects of chilling stress by improving photosynthesis and alleviating oxidative damage, and H 2 O 2 may be a downstream signaling molecule in this process.