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Meichen Liu,Yu Zhao,Baojin Yao,Hui Zhang,Huanyu Guo,Dongyang Hu,Qun Wang 한국유전학회 2014 Genes & Genomics Vol.36 No.5
The most commonly used normalization strategyfor quantitative real-time reverse transcription-polymerasechain reaction (RT-qPCR) is to select a stablereference gene. However, to date, no suitable referencegenes have been identified in sika deer antler tissues. Thus,the aim of this study was to identify the most stable gene ora set of genes to be used as reference genes for RT-qPCRanalysis in sika deer antler tissues. We first selected candidatereference genes using sika deer antler gene expressiondata from an Illumina sequencing platform (Hiseq2000); twenty-one reference genes from the antler tips ofChinese sika deer were selected to test for the normalizationof expression levels during different growth stages. These genes were tested by RT-qPCR and ranked accordingto the stability of their expression using two differentmethods (implemented in geNorm and NormFinder). Based on different algorithms and analytical procedures,our results clearly indicate RPL40 and Gpx as the moststable reference genes of our pool.