A Pilot Study for Developing an Assessment Scale for the Effect of Herbal Medicine in Healthy Children: Open-Label Study with Gami-Jiwhangtang

Bahn Geon-Ho,Kim Chang-Ju,Chung Joo-Ho,Kim Yong-Hee,Paik Eun-Kyung,Park Jae-Hyung The Society of Korean Medicine 2004 대한한의학회지 Vol.25 No.4

Objective : While the demand for herbal medicine has increased continuously, scientific data attesting to pharmacological activity are still insufficient. One important reason, especially in child patients, is the shortage of standardized instruments for clinical research. This study was designed to develop a scale to assess the effect of herbal medicine in children. Methods : The authors chose Gami-jiwhangtang (GJT) as a standard formulation and developed a scale, Bahn's Drug Evaluation Scale (BaDES), for this experiment. Forty-two healthy children, 7 and 8 years old, living in Seoul, Korea, volunteered to use GJT. The experimental group received GJT for 6 weeks, whereas the control group received no medicine. The children's mothers in both groups completed the BaDES on the sixth and twelfth week after GJT was commenced. Results : The experimental group showed a significant improvement in overall physical condition and gastrointestinal function as compared with the control group. Conclusion : These results suggest that BaDES may be a useful assessment tool for measuring the effect of herbal medicine.

Development of a Water-Window X-ray Source Using a Liquid-Nitrogen Jet

B. Ahn,J. Kim,J. Son,B. Kim,D. Kim 한국물리학회 2009 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.54 No.1

As a source for a soft X-ray microscope, a liquid-nitrogen jet has been developed and its radiation characteristics have been investigated for various laser parameters, such as the laser pulse energy and duration. The effects of pre-pulse and multiple pulses have also been stud- ied. The focus has been the N VI 1s2-1s2p transition at 2.88 nm. The investigation shows that the laser intensity for the maximum conversion efficiency scales as Im ∝ 1/T^∝, where ∝ = 0.9 ± 0.15. The study of the pre-pulse effect on the conversion effciency shows that a pre-pulse of only 2 mJ enhances the conversion efficiency by 10 - 15 times for the main pulse of 15 - 60 mJ at a delay of 3 - 6 ns. The photon flux is 1.2 × 10^12 photons / (pulse sr) at a delay of 4 ns for a main pulse of 60 mJ with a pre-pulse of 4 - 8 mJ. The study of the multiple pulse effect was done in the 4-pulse configuration. The enhancement of the radiation compared to the accumulation of the radiation intensity of each pulse was observed at a certain delays between 4 pulses, implying that there exists a proper pulse structure for maximum radiation. As a source for a soft X-ray microscope, a liquid-nitrogen jet has been developed and its radiation characteristics have been investigated for various laser parameters, such as the laser pulse energy and duration. The effects of pre-pulse and multiple pulses have also been stud- ied. The focus has been the N VI 1s2-1s2p transition at 2.88 nm. The investigation shows that the laser intensity for the maximum conversion efficiency scales as Im ∝ 1/T^∝, where ∝ = 0.9 ± 0.15. The study of the pre-pulse effect on the conversion effciency shows that a pre-pulse of only 2 mJ enhances the conversion efficiency by 10 - 15 times for the main pulse of 15 - 60 mJ at a delay of 3 - 6 ns. The photon flux is 1.2 × 10^12 photons / (pulse sr) at a delay of 4 ns for a main pulse of 60 mJ with a pre-pulse of 4 - 8 mJ. The study of the multiple pulse effect was done in the 4-pulse configuration. The enhancement of the radiation compared to the accumulation of the radiation intensity of each pulse was observed at a certain delays between 4 pulses, implying that there exists a proper pulse structure for maximum radiation.

NOVEL ANTICARCINOGENIC COMPOUNDS ISOLATED FROM ARTEMISIA CAPILLARIS

Bahn, Kyeong N.,Lee, Eun J.,Lee, Jung M.,Byun, Jae I.,Park, Sook J.,Yang, Min S.,Ha, Yeong L. Plant Molecular Biology & Biotechnology Research C 1994 Plant molecular biology and biotechnology research Vol.1994 No.

Chloroform fraction of hot-methanol soluble materials from Artemisia capaillaris, which inhibited DMBA-induced mouse skin carcinogenesis (Bahn et al., 1993), was further fractionated into F1 fraction by combination of solvent partition and TLC procedures. From the F1 fraction exhibited a strong cytotoxicity (ED_(50):<0.08vg/ml) for both L1210 and S- 180 mouse cancer cells, achillin and its isomer were identified as the major anticarcinogenic compounds.

Complete mitochondrial genome of the Korean spotted sealPhoca largha(Mammalia, Pinnipedia, Phocidae): Genetic differences betweenP. larghaandPhoca vitulina

Bahn, So Yeong,Choi, Eun Hwa,Jang, Kuem Hee,Kim, Sang Ki,Ryu, Shi Hyun,Hwang, Ui Wook Informa UK (Taylor Francis) 2009 Mitochondrial DNA Vol.20 No.5

NRF2/ARE pathway negatively regulates BACE1 expression and ameliorates cognitive deficits in mouse Alzheimer’s models

Bahn, Gahee,Park, Jong-Sung,Yun, Ui Jeong,Lee, Yoon Jee,Choi, Yuri,Park, Jin Su,Baek, Seung Hyun,Choi, Bo Youn,Cho, Yoon Suk,Kim, Hark Kyun,Han, Jihoon,Sul, Jae Hoon,Baik, Sang-Ha,Lim, Jinhwan,Wakabay National Academy of Sciences 2019 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.116 No.25

<P><B>Significance</B></P><P>Considering that Alzheimer’s disease (AD) is a chronic disease progressing over a long period of time, even a slight increase of <I>BACE1</I> expression may have a profound effect on Aβ accumulation. We describe a previously unknown mechanism that negatively regulates <I>BACE1</I> and <I>BACE1-AS</I> expression and demonstrate its pivotal role in the progression of Aβ and Tau pathologies and cognitive impairment in two mouse models of AD. Given the recent failures of the clinical trials using enzymatic inhibitors of BACE1, it is critical to explore alternative approaches such as down-regulating <I>BACE1</I> and <I>BACE1-AS</I> transcription. Our finding that NRF2 negatively regulates BACE1 and BACE1-AS therefore suggests a potential for disease modification by NRF2-activating phytochemicals or synthetic small molecules in AD.</P><P>BACE1 is the rate-limiting enzyme for amyloid-β peptides (Aβ) generation, a key event in the pathogenesis of Alzheimer’s disease (AD). By an unknown mechanism, levels of <I>BACE1</I> and a <I>BACE1</I> mRNA-stabilizing antisense RNA (<I>BACE1-AS</I>) are elevated in the brains of AD patients, implicating that dysregulation of <I>BACE1</I> expression plays an important role in AD pathogenesis. We found that nuclear factor erythroid-derived 2-related factor 2 (NRF2/NFE2L2) represses the expression of <I>BACE1</I> and <I>BACE1-AS</I> through binding to antioxidant response elements (AREs) in their promoters of mouse and human. NRF2-mediated inhibition of <I>BACE1</I> and <I>BACE1-AS</I> expression is independent of redox regulation. NRF2 activation decreases production of <I>BACE1</I> and <I>BACE1-AS</I> transcripts and Aβ production and ameliorates cognitive deficits in animal models of AD. Depletion of NRF2 increases <I>BACE1</I> and <I>BACE1-AS</I> expression and Aβ production and worsens cognitive deficits. Our findings suggest that activation of NRF2 can prevent a key early pathogenic process in AD.</P>

Different Antigenic Reactivities of Bovine Brain Glutamate Dehydrogenase Isoproteins

Bahn, Jae Hoon,Choi, Soo Young,Hong, Joung Woo,Lee, Byung Ryong,Ahn, Jee-Yin,Jeon, Seong Kyu,Song, Min-Sun,Cho, Sung-Woo 한림대학교 부설 환경ㆍ생명과학연구소 1999 일송 의학ㆍ생명과학 심포지엄 Vol.- No.1

The structural differences between two types of glutamate degydrogenase (GDH) isoproteins (GDH Ⅰ and GDH Ⅱ), homogeneously isolated from bovine brain, were investigated using a biosensor technology and monoclonal antibodies A total of seven monoclonal antibodies raised against GDH Ⅱ were produced, and the anitbodies recognized a single protein band that comigrates with purified GDH Ⅱ on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblot. Of seven anti-GDH Ⅱ monoclonal antibodies tested in the immunoblot analysis, all seven antibodies interacted with GDH Ⅱ, whereas only four antibodies recognized the protein band of the other GDH isoprotein, GDH Ⅰ. When inhibition tests of the GDH isoproteins were performed with the seven anti-GDH Ⅱ monoclonal antibodies, three antibodies inhibited GDH Ⅱ activity, whereas only one antibody inhibited GDH Ⅰ activity. The binding affinity of anit-GDH Ⅱ monoclonal antibodies for GDH Ⅱ(Kp=1.0 nM) determined using a biosensor technology (Pharmacia BIAcore) was fivefold higher than for GDH Ⅰ(Kp=5.3nM). These results, together with epitope mapping analysis, suggest that there may be structural differences between the two GDH isoproteins, in addition to their defferent biochemical properties. Using the anti-GDH Ⅱ antibodies as probes, we also investigated the crossreactivities of brain GDHs from some mammalian and an avian species, showing that the mammalian brain GDH enzymes are related immunologically to each other.

A Pilot Study to Assess the Effect of Gami-Jiwhang-Tang on Cognitive Effects in Healthy Children

Bahn Geon-Ho,Kim Chang-Ju,Chung Joo-Ho,Kim Yong-Hee,Paik Eun-Kyung,Park Jae-Hyung The Society of Korean Medicine 2004 대한한의학회지 Vol.25 No.4

Objective : Treatments for patients with mental retardation and pervasive developmental disorders are not curative, and are designed to help those with disabilities adjust to their environments and daily demands. As clinicians, the present authors tried to find agents with potentially curative properties. Among the numerous herbal formulations available, we chose and assessed Gami-jiwhang-tang (GJT) in the hope that it would improve cognitive development of children. Methods : Subjects were typically-developing healthy, 7- to 8-year-old boys and girls living in Seoul, Korea. The experimental group took GJT for six weeks and was followed up six weeks after discontinuation of GJT. The control group was assessed at the same intervals but did not receive placebos. To measure the effects of GJT, neuropsychological tests and intelligence test were taken before commencing GJT and twelve weeks later. Resulets and Conclusion : For all of the ANOVAs, the treatment by time interaction terms was not significant. However, the experimental group showed the tendency to be progressed in most subscales compared with the control group, especially on performance intelligence, visual organization, and verbal fluency. Conclusion : Although GJT failed to reveal significant improvement in cognition, we remain hopeful about the compound and believe that it should be evaluated by a double-blind, placebo-controlled trial in the future.

INHIBITION OF 7,12-DIMETHYLBENZ[A]ANTHRACENE-INDUCED MOUSE SKIN CARCINOGENESIS BY CHLOROFORM-SOLUBLE MATERIALS FROM ARTEMISIA CAPILLARIS

Bahn, Kyung N.,Lee, Eun J.,Yang, Min S.,Ha, Yeong L. Plant Molecular Biology & Biotechnology Research C 1993 Plant molecular biology and biotechnology research Vol.1993 No.

ISOPERIMETRIC INEQUALITIES AND CONJUGATE POINTS ON LORENTZIAN SURFACES

BAHN, HYOUNGSICK 경북대학교 위상수학 기하학연구센터 1999 硏究論文集 Vol.8 No.-

The h-deformed quantum plane is a counterpart of the q-deformed one in the set of quantum planes which are covariant under those quantum deformations of GL(2) which admit a central determinant. We have investigated the non-commutative geometry of the h-deformed quantum plane. There is a two-parameter family of torsion-free linear connections, a one-parameter sub-family of which are compatible with a skew-symmetric non-degenerate bilinear map. The skew-symmetric map resembles a symplectic 2-form and induces a metric. It is also shown that the extended h-deformed quantum plane is a non-commutative version of the Poincare´ half-plane, a surface of constant negative Gaussian curvature.

Characterization of Water-Filled Ag/AgCl Reference Electrode

Bahn Chi Bum,Oh Sihyoung,Hwang Il Soon,Chung Hahn Sup,Jegarl Sung The Korean Electrochemical Society 2001 한국전기화학회지 Vol.4 No.3

외부 Ag/AgCl 기준 전극은 가압형 및 비등형 경수로 환경에 널리 사용되었다. 전극의 채움 용액 (Siting solution)으로 통상 KCl을 사용하는데, 다공성 지르코니아로 만들어지는 플러그를 통한 Cl 이온의 누설이 전극의 전위차 변동을 유발하는 문제가 있다. 누설로 인한 전위차 변동의 문제를 해결하기 위해 채움 용액으로 순수를 사용하였다 순수를 사용하는 경우 상온에서의 AgCl용해도에 의해 Cl이온의 농도가 결정된다. 붕산과 수산화리튬 혼합용액으로 $288^{\circ}C$에서 전극의 안정성 실험을 실시하였다. 약 일주일간 전위차 변화는 10mV 이내였으며, $288^{\circ}C$와 $240^{\circ}C$에서의 온도 사이클링 시험 전후의 전위차 변화는 15mV 이내였다. 이온의 limiting equivalent conductances와 Agar의 수역학적 이론을 토대로 하여 전극의 TLJP을 계산하였다. 전극 채움 용액 내의 Cl이온 농도를 상온에서 측정한 값으로 보정하여 이론값을 계산할 경우, 실험값과 비교적 잘 일치하는 것을 알 수 있었다. Pressure-balanced external Ag/AgCl electrode has been extensively used for both Pressurized Water Reactor (PWR) and Boiling Water Reactor (PWR) environments. The use of KCI-based buffer solution often becomes the source of electrode potential drift due to slow leakage through its porous plug, typically made of zirconia. It is reported that results of our effort to improve the stability of electrode potential by using high purity water as the filling solution in which $Cl^-$ ion activity can be established and maintained at the solubility of AgCl even with the sustained leakage for a long period. Stability tests have been made in boron and lithium mixture solution at $288^{\circ}C$. The electrode potential remained stable within 10 mV over one week period. And after a thermal cycle between 288 to $240^{\circ}C$ the potential shift of Ag/AgCl electrodes did not exceed 15 mV By using the limiting equivalent ionic conductances and Agar's hydrodynamic theory, the thermal liquid junction potential (TLJP) of the electrode has been predicted. The calculated values for the water-fiued Ag/AgCl electrode potential, in which the chlorine concentration in the filling solution was derived from the measured data at ambient temperature, had a good agreement with the experimental values.