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Oh, Baek-Rock,Hong, Won-Kyung,Heo, Sun-Yeon,Joe, Min-ho,Seo, Jeong-Woo,Kim, Chul Ho Published by Stockton Press on behalf of the Socie 2013 Journal of industrial microbiology & biotechnology Vol.40 No.2
<P>Transcriptome analysis of a K. pneumoniae GEM167 mutant strain derived by irradiation with gamma rays, which exhibited high-level production of ethanol from glycerol, showed that the mutant expressed AdhE at a high level. Ethanol production decreased significantly, from 8.8 to 0.5?g?l(-1), when an adhE-deficient derivative of that strain was grown on glycerol. Bacterial growth was also reduced under such conditions, showing that AdhE plays a critical role in maintenance of redox balance by catalyzing ethanol production. Overexpression of AdhE enhanced ethanol production, from pure or crude glycerol, to a maximal level of 31.9?g?l(-1) under fed-batch fermentation conditions; this is the highest level of ethanol production from glycerol reported to date.</P>