당뇨병 백서의 간세포에서 Glucokinase 활성도 및 유전자 발현에 대한 인슐린의 영향

강성이,팽정령,서광식,안규정,우정택,김성운,양인명,김진우,김영설,김광원,최영길 경희대학교 유전공학연구소 1993 遺傳工學論文集 Vol.5 No.-

목적 당대사의 조절 상태에 따른 생체 변화를 분자 수준에서 이해하고자 식이 조건을 달리한 정상 백서와 화학적으로 유도된 당뇨병 백서의 간조직에서 혈당수준과 인슐린치료 정도에 따라 나타나는 글루코키나제 활성도 및 유전자 발현을 분석하였다. 방법 스트렙토조토신 정맥투여 후 당뇨병의 유발을 확인하고, 인슐린을 1일 3회 3일간 복강내로 투여하여 상태를 안정시킨 후, 인슐린 투여군은 인슐린 투여 6시간이내에 그리고 인슐린 비투여군은 24시간 후 단두하여 채혈하고 복강을 열어 간조직을 채취하였다. 채취한 간조직에서 글루코키나제 활성도는 인산화된 포도당에서 NADH의 형성을 형광분광계로 측정하였으며, 글루코키나제 유전자 mRNA발현은 Northern 분석법을 이용하였다. 성적 정상 백서에서 공복상태와 식이를 섭취한 경우에 간조직의 글루코키나제 효소의 활성은 차이가 없었으나, 글루코키나제 유전자 mRNA 발현은 증가되었다. 당뇨병이 유발된 백서의 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현은 정상 백서에 비하여 낮았다. 인슐린 투쳐 후 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현이 증가되었고, 특히 혈당이 정상화된 경우에서 글루코키나제 유전자의 mRNA 발현이 증가도었다. 결론 인슐린에 의한 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA을 증가를 볼수 있었다. 당뇨병 백서에서 인슐린 투여 후에 혈당조절이 안된 경우 간조직의 글루코키나제 유전자의 mRNA 발현이 증가가 없는 것으로 보아 글루코키나제 mRNA의 발현에는 인슐린 이외의 다른 요소가 관여할 것으로 생각된다. The liver-specific hexokinase isoenzyme, referred to as glucokinase, is thought to play a key reglulatory role in hepatic glucose metabolism. The glucokinase gene is, therefore, of interest both because of its tissue-specific expression and because of the several regulatory processes that can be analyzed. The level of hepatic glucokinase activity appears to be determined essentially by regulation of the rate of enzyme synthesis, with insulin playing a leading role as an inducer. We investigated the role of insulin for the induction of glucokinase in the liver of diabetic rats. Experimental diabetes was induced by injection of streptozotocin 7 days before the experiment. Regular insulin was given by three days intraperitoneal injection at 8-h interval. The glucokinase mRNA in the liver was estimated by Nothern blot assay, as well as by fluorometric enzyme activity assay. Glucokinase activity was not reduced in the liver of normal fasting rats as compared to normal fed rats. And glucokinase activity was reduced in the liver of diabetic rats as compared to normal rats. In diabetic rats treated with insulin, glucokinase enzyme activity were increased. But glucokinase mRNA expression was only increased in normoglycemic diabetic rat with treated with insulin as compared to hyperglycemic rat. These data indicate that insulin stimulates hepatic glucokinase enzyme activity and mRNA expression. But other hormonal or metabolic factors may be contribute to regulation of glucokinase mRNA expression.

과학고 교육과정 운영에 대한 과학교사 및 학생의 인식

서혜애,손정우 한국교육과정학회 2007 교육과정연구 Vol.25 No.2

양극성 장애의 우세삽화와 임상양상 간의 연관성 : 예비연구

서호준,우영섭,채정호,전태연,김광수,박원명 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.5

Objective : This study was performed to fmd clinical implications ofdistinctive pattems in bipolar patients with predominance of depressive episodes or manic episodes and to find possibilities to predict predominant episodes in bipolar disorder. Methods : The recmitment period was between 2002 and 2004, among inpatients who had received psychiatric treatment for bipolar disorder. The data about the number and the polarity of the past episodes were collected through medical records, and Sub-jects were divided into depressive or manic episode predominant groups. The two groups were compared regarding Sociodemo-graphic and clinical variables. Results : 74 patients met the inclusion criteria during the study period. 21 subjects were classified as depressive episode Pre-dominant group (depressive episode group), and 53 subjects as manic episode predominant group (manic episode group). There were no differences in sociodemographic data, the number of total episodes and hypomanic episodes, and onset age. However, 90.5% (n=19) subjects of depressive episode group showed depressive episode as first episode, and 88.7% (n=47) of manicepisode group experienced manic episode as first episode (χ²=42.13, df=1, P<0.00l). Psychotic symptomswere significantly more prominent in the manic episode group (χ²=6.85, df=1, P=0.009), and the number ofsubjects who had suicide attempt experience was higher in the depressive episode group (χ²=7.39, df=1, P=0.007). In the comparison of highest GAF scores during the past year, significant difference was found between the two group (p=0.046), as the mean scores of depressive epi-sode group was 68.337±7.47 and manic episode groupwas 71.51±5.42. Conclusion : The results ofthis study show that there are differences in clinical features according to predominant episodes in bipolar disorder. The type of onset episode appears to be related to predominant episode in this study, and it would be a valid Pa-rameter with therapeutic implication in bipolar patients.

기능적 자기공명 영상법의 이해

서정진,정광우,강형근 全南大醫大 2003 전남의대학술지 Vol.39 No.4

오이의 性發現에 미치는 Ethephon과 Gibberellin의 自葉期 處理 效果

鄭淳宰,具禹書 東亞大學校 1993 東亞論叢 Vol.30 No.1

오이는 性發現에 관한 基礎資料를 얻고져 飛節成種인 四葉과 節成種인 신록다다기, 그 中間形인 聖護院을 공사하여 種子와 崔芽종자의 蛋白質含量의 品種간 차이와 子葉展開期의 ethephon(250ppm)과 Gibberellin(100ppm)處理에 의한 蛋白質 含量과 peroxidase活性의 變化, 蛋白質 banding pattern 및 perosidase isoenzyme의 banding pteern의 變化 關係를 調査하여 다음과 같은 結果를 얻었다. 1) 種子와 崔牙種子인 蛋白質含量, 蛋白質 banding pattern 및 peroxidase의 banding pattern에서 飛節成과 節成品種간에 뚜렷한 차이를 볼 수 없었다. 2) 子葉期의 蛋白質 含量과 蛋白質 banding pattern에서는 3品種 모두 ethephon處理區에서 蛋白質含量이 높았고, 蛋白質 banding pattern에서는 處理 後 日數가 지남에 따라 變異를 보이다가 處理後 5日째 四葉의 ethephon 處理區의 Rf 0.33-0.34의 位置에 强度높은 band가 形成되었으며, 聖護院, 신록다다기의 無處理區에서 形成된 band의 位置와 强度가 同一하였다. 3) 子葉期의 peroxidase 活性 및 peroxidase banding pattern에서는 ethephon處理區에서 3品種 모두 peroxidase activity가 높게 나타났으며, ethephon 處理後 1일째는 3品種 모두 거의 같은 band의 樣相을 나타내었고, 處理 後 3일째는 變化의 樣相을 보이다가 處理後 5일째는 四葉의 ethephon 處理區와 聖護院, 신록다다기의 control區가 거의 같은 banding pattern을 나타내었다. This experiment was conducted to obtain the basic information the patterns of change in protein contents, peroxidase activity and of sex expression between three cucumber varieties(monoecious type;Sayeupl gynoecious type; Sinrokdadaki, intermediate type; Seonghowon) by treating ethephon with 250ppm and gibberellin with 100ppm at the maturing stage of their cotyledons. The patterns of changes in protein contents and peroxidase activity were analyzed using disk polyacrylamide gel electrophoresis and the results are summarized as follows. 1. No difference was found in changes of the protein contents, band pattern of peroxidase and iso peroxidase activities between mature seeds and germinating seeds of Sayeup(monoecious type) and Sinrokdadaki(gynoecious type). 2. High protein contents were obtained in cotyledons of all varieties treated with ethephon, and a variety of band patterns of protein isolated were shown on the gel during the initial stages after ethephon treatment. After 5 days of ethephon treatment, however, a strong band was observed at the Rf value of 0.33-0.34 on the gel analyzed from the proteins isolated from Sayeup cotyledons with etyephon treatment and the band intensity of their proteins was similar to ones from Seonghowonand Sinrokdadaki with no ethephon treatment. 3. As a result of gel analysis, high activity and strong band of peroxidase isolated from ethephon treated carieties was examined. Similar band patterns of proteins isolated were observed in the cotyledons of all carieties used after one day of ethephon treatment. After 3 days of ethephon treatment, all varieties showed various band patterns, while after 5 days, the band pattern of proteins isolated from ethephon treated Sayeup similar one of proteins from both Seonghowon and Sinrokdadaki with no ethephon.

개에서 발생한 이혈종의 임상적 관리

이우철,윤기영,서경원,최호정,정성목,송근호,조종기,박성준 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2012 動物醫科學硏究誌 Vol.19 No.1

A 14-year-old male pomeranian was present with swollen left ear. The swelling occurred from base to apex of pinna. Otitis externa persisted for two years. Hematological and serum biochemistry values were not remarkable. The surgery treatment was the most appropriate method among indwelling drain, surgical, and steroid treatment in the aural hematoma because the dog was very sensitive to touching pinna and the size of aural hematoma. Longitudinal incision was applied after general anesthesia. Cefovecin was injected to prevent secondary infection. The outcome of surgery was good. There was no evidence of recurrence for one year.

참외의 接木裁培에 관한 硏究

鄭淳宰,具禹書,鄭景泰 東亞大學校 大學院 1992 大學院論文集 Vol.17 No.-

Experiments wer conducted to investigate of rootstocksk on the growth, yield and quality of oriental melon cultivars and to find out problems associated with successful growing of grafted seedlings. Major results can be summarized as follows. 1. The percentage of healthy plants, sintozwa and chamtozwa were 100, while hongtozwa and chambak were less than 11.8. 2. The growth of oriental melon was promoted by non-grafted and sintozwa root stock. 3. Soluble solid contents of non-grafted and chamtozwa rootstock were hight than sintozwa rootstock. 4. Glucose and Fructose contents of sintozwa rootstock was hihter than chamtozwa rootstock. Whereas, sucrose contents of non-grafted was hiher than sintozwa, chamtozwa rootstock. 5. Grafting onto sintozwa rootstock increased the contents of most mineral element except Ca in the leaves.

폐광산 주변의 목본 식생 현황 및 오염 토양에 대한 목본 종자의 발아 특성

서경원,손요환,김래현,구진우,노남진,경지현,김정규 한국환경농학회 2006 한국환경농학회지 Vol.25 No.1

제한효소 DNA 분석법에 의한 국내분리 렙토스피라균의 동정

장우현, 김석용, 서정선 忠北大學校 遺傳工學硏究所 1988 遺傳工學硏究 Vol.2 No.1

본문참고

조기 발병 양극성 장애 환자의 임상적 특징 : 후향적 의무기록 조사 연구

우영섭,박민현,서호준,채정호,전태연,박원명 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.5

Objective : Bipolar disorder is a disease with diverse clinical profiles and outcomes. In recent years, it is suggested that if bipolar disorder occurs early in one's life, functional and symptomatic prognoses are poor. The prognoses include severe Symp-toms, frequent psychotic symptoms, comorbidity ofmental illnesses, slow improvement of the symptoms, and high suicide rate. We investigated the clinical characteristics of early onset bipolar inpatients. Method : The subjects of this study were selected from the patients who were discharged after hospitalization between January 1, 2001 and May 31, 2005 and diagnosed with bipolar disorder according to DSM-IV criteria. These patients were examined for the following data ; the type of bipolar disorder, comorbid psychiatric disorders, the age at onset of the illness, the acuteness of the disorder, and the duration of the disorder. The presence of four major clinical psychopathology, rapid cycling, Psychotic symptoms, comorbid psychiatric disorders, and suicide attempt, was also examined. The patients whose onset of the illness was before the age of 19 were classified as the early onset group and after the age of 19 were classified as the late onset group. Data on demographics, family histories, four major clinical psychopathologies and other clinical variables were compared between the early onset group and the late onset group. Results : Of the 53 patients, 19 patients belonged to the early onset group. There was higher incidence ofpsychotic Symp-toms in the early onset group than in the late onset group, and the difference was statistically significant according to the χ² - test (84.2% vs 44.1%, P=0.005). The incidence of comorbid psychiatric disorders was higher in the early onset group than in the late onset group, and the difference was also statistically significant according to the χ²-test (52.6% vs 23.5%, P=0.032).However, there was no statistically significant difFerence between the groups regarding the incidence of rapid cycling and suicide attempt. The result of logistics regression showed correlation of psychotic symptoms (0R=6.756 ; 1.655≤95% CI≤27.580) and comorbid psychiatric disorders (0R=3.611 ; 1.088≤95% CI≤11.984) with the early onset group. Conclusion : Early onset of bipolar disorder is related to the manifestation of psychotic symptoms and frequently accom-panies comorbid psychiatric disorders. The results of this study will help understand the mechanism of the onset of complexbipolar disorder and estimate the prognosis.