Assessment of technological characteristics and microbiological quality of marinated turkey meat with the use of dairy products and lemon juice

Augustyńska-Prejsnar Anna,Hanus Paweł,Sokołowicz Zofia,Kačániová Miroslava 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.12

Objective: The aim of this study was to evaluate the effect of marinating turkey meat with buttermilk and acid whey on the technological traits and microbiological quality of the product.Methods: Slices of turkey meat muscles were marinated for 12 hours in buttermilk (n = 30), acid whey (n = 30) and comparatively, in lemon juice (n = 30). The control group (n = 30) consisted of unmarinated slices of turkey breast muscles. Physical parameters (pH, water holding capacity, colour L*a*b*, shear force, weight loss) were assessed and quantitative and qualitative microbiological evaluation of raw and roasted products was performed. The microbiological parameters were determined as the total viable counts of mesophilic aerobic bacteria, of the Enterobacteriaceae family, and Pseudomonas spp. Bacterial identification was performed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.Results: Marinating turkey meat in buttermilk and whey compared to marinating in lemon juice and the control sample resulted in a higher (p<0.05) degree of yellow color saturation (b*) and a reduction (p<0.05) in the number of mesophilic aerobic bacteria, Pseudomonas spp. and Enterobacteriaceae family as well as the number of identified mesophilic aerobic bacteria in both raw and roasted samples. The lowest (p<0.05) shear force values were found in products marinated in whey.Conclusion: The use of buttermilk and acid whey as a marinade for meat increases the microbiological safety of the product compared to marinating in lemon juice, while maintaining good technological features of the product. Objective: The aim of this study was to evaluate the effect of marinating turkey meat with buttermilk and acid whey on the technological traits and microbiological quality of the product. Methods: Slices of turkey meat muscles were marinated for 12 hours in buttermilk (n = 30), acid whey (n = 30) and comparatively, in lemon juice (n = 30). The control group (n = 30) consisted of unmarinated slices of turkey breast muscles. Physical parameters (pH, water holding capacity, colour L*a*b*, shear force, weight loss) were assessed and quantitative and qualitative microbiological evaluation of raw and roasted products was performed. The microbiological parameters were determined as the total viable counts of mesophilic aerobic bacteria, of the Enterobacteriaceae family, and Pseudomonas spp. Bacterial identification was performed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Results: Marinating turkey meat in buttermilk and whey compared to marinating in lemon juice and the control sample resulted in a higher (p<0.05) degree of yellow color saturation (b*) and a reduction (p<0.05) in the number of mesophilic aerobic bacteria, Pseudomonas spp. and Enterobacteriaceae family as well as the number of identified mesophilic aerobic bacteria in both raw and roasted samples. The lowest (p<0.05) shear force values were found in products marinated in whey. Conclusion: The use of buttermilk and acid whey as a marinade for meat increases the microbiological safety of the product compared to marinating in lemon juice, while maintaining good technological features of the product.

A "deformable section" model for the dynamics of suspension bridges -Part II: Nonlinear analysis and large amplitude oscillations

Sepe, Vincenzo,Diaferio, Mariella,Augusti, Giuliano Techno-Press 2003 Wind and Structures, An International Journal (WAS Vol.6 No.6

The classical two-degree-of-freedom (2-d-o-f) "sectional model" is of common use to study the dynamics of suspension bridges. It takes into account the first pair of vertical and torsional modes of the bridge and describes well global oscillations caused by wind actions on the deck, yielding very useful information on the overall behaviour and the aerodynamic and aeroelastic response; however, it does not consider relative oscillations between main cables and deck. On the contrary, the 4-d-o-f model described in the two Parts of this paper includes longitudinal deformability of the hangers (assumed linear elastic in tension and unable to react in compression) and thus allows to take into account not only global oscillations, but also relative oscillations between main cables and deck. In particular, when the hangers go slack, large nonlinear oscillations are possible; if the hangers remain taut, the oscillations remain small and essentially linear: the latter behaviour has been the specific object of Part I (Sepe and Augusti 2001), while the present Part II investigates the nonlinear behaviour (coexisting large and/or small amplitude oscillations) under harmonic actions on the cables and/or on the deck, such as might be generated by vortex shedding. Because of the discontinuities and strong nonlinearity of the governing equations, the response has been investigated numerically. The results obtained for sample values of mechanical and forcing parameters seems to confirm that relative oscillations cannot a priori be excluded for very long span bridges under wind-induced loads, and they can stimulate a discussion on the actual possibility of such phenomena.

A deformable section model for the dynamics of suspension bridges -Part I : Model and linear response

Sepe, Vincenzo,Augusti, Giuliano Techno-Press 2001 Wind and Structures, An International Journal (WAS Vol.4 No.1

The classical two-degree-of-freedom (2-d-o-f) "sectional model" is currently used to study the dynamics of suspension bridges. Taking into account the first pair of vertical and torsional modes of the bridge, it describes well global oscillations caused by wind actions on the deck and yields very useful information on the overall behaviour and the aerodynamic and aeroelastic response, but does not consider relative oscillation between main cables and deck. The possibility of taking into account these relative oscillations, that can become significant for very long span bridges, is the main purpose of the 4-d-o-f model, proposed by the Authors in previous papers and fully developed here. Longitudinal deformability of the hangers (assumed linear elastic in tension and unable to react in compression) and external loading on the cables are taken into account: thus not only global oscillations, but also relative oscillations between cables and deck can be described. When the hangers go slack, large nonlinear oscillations are possible; if the hangers remain taut, the oscillations are small and essentially linear. This paper describes the model proposed for small and large oscillations, and investigates in detail the limit condition for linear response under harmonic actions on the cables (e.g., like those that could be generated by vortex shedding). These results are sufficient to state that, with geometric and mechanical parameters in a range corresponding to realistic cases of large span suspension bridges, large relative oscillations between main cables and deck cannot be excluded, and therefore should not be neglected in the design. Forthcoming papers will investigate more general cases of loading and dynamic response of the model.

Hyperthermal Collisions of Bromotoluene Molecular Cations at Self-Assembled Monolayer Surfaces

Sung-Chan Jo,Rodinei Augusti,R. Graham Cooks 사단법인 한국질량분석학회 2011 Mass spectrometry letters Vol.2 No.1

Hyperthermal ion/surface collisions of bromotoluene molecular ions were studied using perfluorinated (F-SAM) andhydroxyl-terminated (OH-SAM) self-assembled monolayer surfaces in a tandem mass spectrometer with BEEQ geometry. Theisomers were differentiated by ion abundance ratios taken from surface-induced dissociation (SID). The dissociation rate followedthe order of ortho>meta>para isomers. The peak abundance ratio of m/z 51 to m/z 65 showed the best result to discern theisomers, while the other ratios would effectively serve the same purpose as well. A dissociation channel leading to tolylium ionwas suggested to be responsible for the pronounced isomeric differentiability. The capability of SID to provide high-energy activationwith narrow internal energy distribution may have channeled the reaction into the specific dissociation pathway, also facilitatingsmall difference in reaction rates to be effective in the spectral time window of this experiment. All the molecular ions experiencedreactive collisions with the F-SAM surface leading to transhalogenation products, where a fluorine atom from the surface replacesthe bromine of the projectile. This reactive collision was dependant on the laboratory collision energy occurring in ca. 40~75 eV range.

Hyperthermal Collision-induced Dissociation of Bromotoluene Radical Cations at Self-Assembled Monolayer Surfaces

( Sung-chan Jo ),( Rodinei Augusti ),( R. Graham Cooks ) 한국질량분석학회 2011 Mass spectrometry letters Vol.2 No.1

Hyperthermal ion/surface collisions of bromotoluene radical cations were studied using perfluorinated (F-SAM) and hydroxyl-terminated (OH-SAM) self-assembled monolayer surfaces in a tandem mass spectrometer with BEEQ geometry. The isomers were differentiated by ion abundance ratios taken from surface-induced dissociation (SID). The dissociation rate followed the order of ortho > meta > para isomers. The peak abundance ratio of m/z 51 to m/z 65 showed the best result to discern the isomers. A dissociation channel leading to tolylium ion was suggested to be responsible for the pronounced isomeric differences. The capability of SID to provide high-energy activation with narrow internal energy distribution may have channeled the reaction into the specific dissociation pathway, also facilitating small differences in reaction rates to be effective in the spectral time window of this experiment. All of the molecular ions experiencing reactive collisions with the F-SAM surface undergo transhalogenation, in which a fluorine atom on the surface replaces the bromine in the incoming ions. This reactive collision was dependent on the laboratory collision energy occurring in ca. 40.75 eV range.

Hyperthermal Collision-induced Dissociation of Bromotoluene Radical Cations at Self-Assembled Monolayer Surfaces

Jo, Sung-Chan,Augusti, Rodinei,Cooks, R. Graham Korean Society for Mass Spectrometry 2011 Mass spectrometry letters Vol.2 No.1

Hyperthermal ion/surface collisions of bromotoluene radical cations were studied using perfluorinated (F-SAM) and hydroxyl-terminated (OH-SAM) self-assembled monolayer surfaces in a tandem mass spectrometer with BEEQ geometry. The isomers were differentiated by ion abundance ratios taken from surface-induced dissociation (SID). The dissociation rate followed the order of ortho > meta > para isomers. The peak abundance ratio of m/z 51 to m/z 65 showed the best result to discern the isomers. A dissociation channel leading to tolylium ion was suggested to be responsible for the pronounced isomeric differences. The capability of SID to provide high-energy activation with narrow internal energy distribution may have channeled the reaction into the specific dissociation pathway, also facilitating small differences in reaction rates to be effective in the spectral time window of this experiment. All of the molecular ions experiencing reactive collisions with the F-SAM surface undergo transhalogenation, in which a fluorine atom on the surface replaces the bromine in the incoming ions. This reactive collision was dependent on the laboratory collision energy occurring in ca. 40.75 eV range.

HPLC-DAD Phenolic Profiling and In Vitro Antioxidant Activities of Three Prominent Nigerian Spices

Olufunmilayo Sade Omoba,Aderonke Ibidunni Olagunju,Sule Ola Salawu,Aline Augusti Boligon 한국식품영양과학회 2019 Preventive Nutrition and Food Science Vol.24 No.2

Spices and herbs have antioxidant, anti-inflammatory, and anti-microbial properties, amongst others. These characteristics are attributable to their composition, such as high polyphenol and flavonoid contents that are responsible for their antioxidative properties. Methanolic extracts of Ocimum basilicum (OB), Xylopia aethiopica (XA), and Piper guineensis (PG) were evaluated to profile their phenolic compounds and in vitro antioxidant properties. High performance liquid chromatography with diode-array detection phenolic compounds profiling revealed that quercetin, quercitrin, and isoquercitrin are the most prevalent phenolic compound in OB, XA, and PG, respectively. All the extracts possessed good antioxidant activity. XA showed the highest total phenolic content of 29.50 ㎎ gallic acid equivalents/g, a total flavonoid content of 21.17 ㎎ quercetin equivalents/g, 2,2-diphenyl-1-picrylhydrazyl and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging abilities of 29% and 88.23%, respectively, and a nitric oxide scavenging activity of 44.13 ㎎/g. Thus, the XA methanolic extract demonstrated a high content of phenolic compounds and significant antioxidative properties, with prospective potency to prevent oxidative damage and promote better cardiovascular health.

Antioxidant and Anticholinesterase Potential of Two Nigerian Bitter Yams Using a Simulated Gastrointestinal Digestion Model and Conventional Extraction

Salawu, Sule Ola,Ajiboye, Praise Blessing,Akindahunsi, Akintunde Afolabi,Boligon, Aline Augusti The Korean Society of Food Science and Nutrition 2017 Preventive Nutrition and Food Science Vol.22 No.2

The purpose of this study was to evaluate the antioxidant and anticholinesterase activities of yellow and white bitter yams from South Western Nigeria using methanolic extraction and simulated gastrointestinal digestion models. The phenolic compounds in the bitter yam varieties were evaluated by high performance liquid chromatography with a diode array detector (HPLC-DAD). The total phenolic content of the bitter yams was measured by the Folin-Ciocalteu method, reductive potential by assessing the ability of the bitter yam to reduce $FeCl_3$ solution, and the antioxidant activities were determined by the 2,2-diphenyl-1-picrylhydrazyl radical ($DPPH^{\cdot}$) scavenging activity, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation ($ABTS^{{\cdot}+}$) scavenging activity, nitric oxide radical ($NO^{\cdot}$) scavenging ability, hydroxyl radical scavenging ability, and ability to inhibit $Fe^{2+}$-induced lipid oxidation. The HPLC-DAD analysis revealed the presence of some phenolic compounds in the studied bitter yam varieties, with varying degree of quantitative changes after cooking. The antioxidant indices (total phenolic content, total flavonoid content, reducing power, $DPPH^{\cdot}$ scavenging activity, $ABTS^{{\cdot}+}$ scavenging activity, and $NO^{\cdot}$ scavenging activity) were higher in the simulated gastrointestinal digestion model compared to the methanolic extract, with the in vitro digested cooked white bitter yam ranking higher. Similarly, the in vitro digested yams had a higher inhibitory action against lipid oxidation compared to the methanolic extracts, with the cooked white bitter yam ranking high. The methanolic extracts and in vitro enzyme digests showed no acetylcholinesterase inhibitory abilities, while methanolic extracts and the in vitro enzyme digest displayed some level of butyrylcholinesterase inhibitory activities. Therefore the studied bitter yams could be considered as possible health supplements.

In Vitro Antioxidant Activity and Effect of Parkia biglobosa Bark Extract on Mitochondrial Redox Status

Kayode Komolafe,Tolulope Mary Olaleye,Olaposi Idowu Omotuyi,Aline Augusti Boligon,Margareth Linde Athayde,Akintunde Afolabi Akindahunsi,Joao Batista Teixeira da Rocha 사단법인약침학회 2014 Journal of Acupuncture & Meridian Studies Vol.7 No.4

Aqueous-methanolic extract of Parkia biglobosa bark (PBB) was screened for its polyphe- nolic constituents, in vitro antioxidant activity, and effect on mitochondria redox status. The in vitro antioxidant activity was assessed by using the scavenging abilities and the reducing powers of 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) and 2,2 0 -azino-bis(3- ethylbenzothiazoline-6-sulfonic acid) (ABTS) diammonium salt radical cation against Fe 3þ . Subsequently, the ability of PBB to inhibit lipid peroxidation induced by FeSO 4 (10 mm) and its metal-chelating potential were investigated. The effects of the extract on basal reactive oxygen species (ROS) generation and on the mitochondrial membrane potential (DJm) in isolated mitochondria were determined by using 2 0 , 7 0 -dichlorodihy- drofluorescin (DCFH) oxidation and safranin fluorescence, respectively. PBB mitigated the Fe(II)-induced lipid peroxidation in rat tissues and showed dose-dependent scav- enging of DPPH (IC 50 : 98.33 ± 10.0 mg/mL) and ABTS. (trolox equivalent antioxidant concentration, TEAC value = 0.05), with considerable ferric-reducing and moderate metal-chelating abilities. PBB caused slight decreases in both the liver and the brain mitochondria potentials and resulted in a significant decrease (p < 0.001) in DCFH oxida- tion. Screening for polyphenolics using high-performance liquid chromatography coupled to a diode array detector (HPLC-DAD) revealed the presence of caffeic acid, gallic acid, catechin, epigalocatechin, rutin, and quercetin. These results demonstrate for the first time the considerable in vitro antioxidant activity and favorable effect of PBB on mito- chondria redox status and provide justification for the use of the plant in ethnomedicine.

Effect of Two Ginger Varieties on Arginase Activity in Hypercholesterolemic Rats

Ayodele Jacob Akinyemi,Ganiyu Oboh,Adedayo Oluwaseun Ademiluyi,Aline Augusti Boligon,Margareth Linde Athayde 사단법인약침학회 2016 Journal of Acupuncture & Meridian Studies Vol.9 No.2

Recently, ginger has been used in traditional Chinese medicine as an herbal therapy for treating several cardiovascular diseases, however, information on its mechanism of action is limited. The present study assessed the effect of two ginger varieties (Zingiber officinale and Curcuma longa) on the arginase activity, atherogenic index, levels of liver thiobarbituric acid reactive substances (TBARSs), and plasma lipids in rats fed with a high-cholesterol (2%) diet for 14 days. Following the treatment period, it was found that feeding a high-cholesterol diet to rats caused significant (p < 0.05) increases in arginase activity, atherogenic index, levels of TBARS, total cholesterol (TC), triglycerides (TGs), and low-density lipoprotein cholesterol (LDL-C) with a concomitant decrease in highdensity lipoprotein cholesterol (HDL-C). However, both ginger and turmeric (2% and 4%) caused significant (p < 0.05) decreases in arginase activity and the atherogenic index, and prevented hypercholesterolemia by decreasing the TC, TGs, and LDL-C while increasing the HDL-C when compared with the controls. In conclusion, dietary supplementation with both types of rhizomes (ginger and turmeric) inhibited arginase activity and prevented hypercholesterolemia in rats that received a high-cholesterol diet. Therefore, these activities of ginger and turmeric represent possible mechanisms underlying its use in herbal medicine to treat several cardiovascular diseases.