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Genetics of resistance to Cry1C toxin from Bacillus thuringiensis in Spodoptera litura (Fab.)
Atul S.Thakare,Umesh P. BARKHADE,Vrushali N. PAWADE,Shivcharan M. THAKARE 한국곤충학회 2010 Entomological Research Vol.40 No.3
The present study was undertaken to determine the genetics of Cry1C resistance in Spodoptera litura. Selection of S. litura (Fab.) with Cry1C was done for eight generations to develop resistance. Reciprocal crosses between resistant and susceptible populations were made to understand the population genetics of Cry1C resistance in S. litura. Generation wise selection with Cry1C was evaluated for resistance development in S. litura. The LC50 of Cry1C was 0.14 µg/cm2 for the first selected generation and it increased to 23.98 µg/cm2 after eight selected generations, which is a 285.47-fold increase in resistance compared with the susceptible strain. The estimated realized heritability (h2) after eight generations of selection with Cry1C insecticidal protein was 0.44. The number of generations required for the tenfold increase in LC50 (1/R) was estimated to be 3.33. Response to Cry1C selection in S. litura was 0.30, the estimated selection differential was 0.69 and the pheonotypic standard deviation (dP) was 0.24. Reciprocal crosses between Cry1C resistant and susceptible strain of S. litura showed autosomal resistance.
Sarita NEHARE,Basweshwar S. GHODKI,Gajanan K. LANDE,Vrushali PAWADE,Atul S. THAKARE 한국곤충학회 2010 Entomological Research Vol.40 No.1
Leaf-dip assay of Plutella xylostella against indoxacarb showed that the concentration that produced 50% mortality (LC50) of indoxacarb ranged from 20.1 to 11.9 ppm, with highest in Nasik and lowest levels in Coimbatore strains. In selection studies, the LC50 of indoxacarb was 18.5 ppm at generation 1 (G1), which increased to 31.3-fold (167.8 ppm) resistance after ten exposed generations (G10) as compared to unexposed. The LC50 of quinalphos was 74.4 ppm, which increased to 10.0-fold (631.5 ppm) resistance after G10. The LC50 of cypermethrin resistant strain resulted in an 11.5-fold increase in resistance after G10. In P. xylostella, heritability (h2) after ten generations of selection was estimated at 0.4. The number of generations required for tenfold increase in LC50 (1/R) were 6.7. The response to indoxacarb selection in P. xylostella was 0.2 and the selection differential was estimated as 0.4. The phenotypic standard deviation was 0.2. Reciprocal crosses between indoxacarb-resistant and susceptible strains showed that the inheritance of indoxacarb resistance was autosomal. The degree of heritability (DLC) (0.4, 0.4) indicated incomplete recessive inheritance of indoxacarb resistance.