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Biochemical analysis and synergistic suppression of indoxacarb resistance in Plutella xylostella L.
Sarita Nehare,M.P. Moharil,B.S. Ghodki,G.K. Lande,K.D. Bisane,A.S. Thakare,U.P. Barkhade 한국응용곤충학회 2010 Journal of Asia-Pacific Entomology Vol.13 No.2
Indoxacarb was treated to Plutella xylostella for 10 generations to develop a resistant strain and biochemical analysis of indoxacarb resistance in different tissues of P. xylostella was carried out. Biochemical analysis found maximum esterase activity in gut homogenates of indoxacarb resistant strains followed by whole body and cuticle homogenates. In gut homogenates of indoxacarb resistant strains, maximum increase in esterases was found as compared to the unselected strain. Acetylcholineesterase activity was higher in head homogenates of the resistant strain than in the unselected strain. Glutathione-S-transferase activity was highest in whole body homogenates. However, maximum increase was found in gut homogenates of indoxacarb resistant strains over the unselected. Induced resistance was suppressed using known synergists. Maximum synergism occurred using diethyl-maleate (DEM), followed by triphenyl phosphate (TPP).
Sarita NEHARE,Basweshwar S. GHODKI,Gajanan K. LANDE,Vrushali PAWADE,Atul S. THAKARE 한국곤충학회 2010 Entomological Research Vol.40 No.1
Leaf-dip assay of Plutella xylostella against indoxacarb showed that the concentration that produced 50% mortality (LC50) of indoxacarb ranged from 20.1 to 11.9 ppm, with highest in Nasik and lowest levels in Coimbatore strains. In selection studies, the LC50 of indoxacarb was 18.5 ppm at generation 1 (G1), which increased to 31.3-fold (167.8 ppm) resistance after ten exposed generations (G10) as compared to unexposed. The LC50 of quinalphos was 74.4 ppm, which increased to 10.0-fold (631.5 ppm) resistance after G10. The LC50 of cypermethrin resistant strain resulted in an 11.5-fold increase in resistance after G10. In P. xylostella, heritability (h2) after ten generations of selection was estimated at 0.4. The number of generations required for tenfold increase in LC50 (1/R) were 6.7. The response to indoxacarb selection in P. xylostella was 0.2 and the selection differential was estimated as 0.4. The phenotypic standard deviation was 0.2. Reciprocal crosses between indoxacarb-resistant and susceptible strains showed that the inheritance of indoxacarb resistance was autosomal. The degree of heritability (DLC) (0.4, 0.4) indicated incomplete recessive inheritance of indoxacarb resistance.