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        Studies on encapsulation of Rifampicin and its release from chitosan-dextran sulfate capsules

        M. Geetha Devi,Susmita Dutta,Ashraf Talib Al Hinai,S. Feroz 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.1

        Biodegradable capsules of size around 350 nm were prepared by layer-by-layer (L-b-L) assembly of oppo-sitely charged chitosan and dextran sulfate on silica particles and the subsequent removal of template. The resultingcapsules were loaded with rifampicin, an anti-tuberculosis drug under modest conditions, as demonstrated by scan-ning electron microscopy (SEM). Maximum encapsulation of rifampicin was found to be about 82 µg at 25 oC and pHof 3. Release studies were done in-vitro mode by semiautomatic release protocol, with different pH solutions in waterand phosphate buffered saline (PBS). The microcapsules exhibited a slow and sustained release over 72 hours and max-imum release was obtained at a pH of 1.2 in water and a pH of 7.4 in PBS. The size of silica particle was analyzed bydynamic light scattering method. Scanning electron microscopy (SEM) measurements showed the surface morphol-ogy of the hollow capsules. UV spectroscopy was employed to monitor the drug release processes in both solutions. The kinetics of drug release mechanism was studied using Ritger-Peppas and Higuchi models.

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