Tolerogenic Dendritic Cells Reduce Airway Inflammation in a Model of Dust Mite Triggered Allergic Inflammation

Luciana S. Aragão-França,Viviane C. J. Rocha,Andre Cronemberger-Andrade,F. H. B. Costa,José Fernandes Vasconcelos,Daniel Abensur Athanazio,Daniela Nascimento Silva,E. S. Santos,Cássio Santana Meira,C. 대한천식알레르기학회 2018 Allergy, Asthma & Immunology Research Vol.10 No.4

Purpose: The use of tolerogenic dendritic cells (TolDCs) to control exacerbated immune responses may be a prophylactic and therapeutic option for application in autoimmune and allergic conditions. The objective of this work was to evaluate the effects of TolDC administration in a mouse model of allergic airway inflammation caused by mite extract. Methods: Mouse bone marrow-derived TolDCs were induced by incubation with granulocyte- macrophage colony-stimulating factor (GM-CSF) and dexamethasone, and then characterized by flow cytometry and cytokine production by enzyme- linked immunosorbent assay (ELISA). For the in vivo model of Blomia tropicalis-induced allergy, mice transplanted with antigen-pulsed TolDCs were sensitized intraperitoneally with B. tropicalis mite extract (BtE) adsorbed to aluminium hydroxide. After challenge by nasal administration of BtE, bronchoalveolar lavage fluid (BALF), lungs, spleen and serum were collected for analysis. Results: Induction of TolDCs was efficiently achieved as shown by low expression of major histocompatibility complex (MHC) II, programmed death-ligand (PD-L) 2 and pro-inflammatory cytokine production, and up-regulation of interleukin (IL)-10, upon LPS stimulation in vitro. Transplantation of 1 or 2 doses of BtE-pulsed TolDCs reduced the number of inflammatory cells in BALF and lungs as well as mucus deposition. Moreover, compared to saline-injected controls, TolDC-treated mice showed lower serum levels of anti-BtE immunoglobulin E (IgE) antibodies as well as reduced Gata3 and IL-4 gene expression in the lungs and decreased IFN-γ levels in the supernatant of splenocyte cultures Transplantation of TolDCs increased the percentage of the regulatory T cells in the spleen and the lungs. Conclusions: Preventive treatment with TolDCs protects against dust mite-induced allergy in a mouse model, reinforcing the use of tolerogenic dendritic cells for the management of allergic conditions.

Mechanical properties evaluatin of alumina-zirconia-spinel ceramic composites using ultrasonic pulse velocity

B. M. Cunha,R. M. P. B., Oliveira,V. T. S. Aragão,C. O. D. Martins 한양대학교 세라믹연구소 2019 Journal of Ceramic Processing Research Vol.20 No.2

The evaluation of mechanical properties is essential to the performance guarantee of the material. However, this process is verycomplex for ceramic materials due to the variety of defects of such material. The aim of this work was to investigate the useof ultrasonic velocity measurements to the determination of mechanical properties in ceramic composites. To accomplish this,seven batches of alumina/zirconia, alumina/spinel and alumina/zirconia/spinel refractories were grinded, uniaxially pressed at100 MPa and sintered at 1550 oC. The evaluation of ultrasonic pulse velocity (UPV) was performed in commercial equipmentwith a transducer of 5 MHz. The results showed that the addition of zirconia and spinel to the alumina matrix increased thecompression resistance of the matrix in 75%. Furthermore, exponential equations relating mechanical properties and UPVwere found and it was observed the existence of an intrinsic relation between these parameters.

Push-out bond strength of a self-adhesive resin cement used as endodontic sealer

Eduardo Diogo Gurgel-Filho,Felipe Coelho Lima,Vicente de Paula Aragão Saboia,Tauby de Souza Coutinho-Filho,Aline de Almeida Neves,Emmanuel João Nogueira Leal da Silva 대한치과보존학회 2014 Restorative Dentistry & Endodontics Vol.39 No.4

Objectives: The aim of the present study was to investigate the bond strength of RelyX Unicem (3M) to root canal dentin when used as an endodontic sealer. Materials and Methods: Samples of 24 single-rooted teeth were prepared with Gates Glidden drills and K3 files. After that, the roots were randomly assigned to three experimental groups (n = 8) according to the filling material, (1) AH Plus (Dentsply De Trey GmbH)/ Gutta-Percha cone; (2) Epiphany SE (Pentron)/Resilon cone; (3) RelyX Unicem/Gutta- Percha cone. All roots were filled using a single cone technique associated to vertical condensation. After the filling procedures, each tooth was prepared for a push-out bond strenght test by cutting 1 mm-thick root slices. Loading was performed on a universal testing machine at a speed of 0.5 mm/min. One-way analysis of variance and Tukey test for multiple comparisons were used to compare the results among the experimental groups. Results: Epiphany SE/Resilon showed significantly lower pushout bond strength than both AH Plus/Gutta-Percha and RelyX Unicem/Gutta-Percha (p < 0.05). There was no significant difference in bond strength between AH Plus/Gutta- Percha and RelyX Unicem/Gutta-Percha (p > 0.05). Conclusions: Under the present in vitro conditions, bond strength to root dentin promoted by RelyX Unicem was similar to AH Plus. Epiphany SE/Resilon resulted in lower bond strength values when compared to both materials.

Colorimetric and Chlorophyll Fluorescence Assays for Fast Detection and Selection of Transgenic Events of Cotton, Cowpea, Soybean and Common Bean Expressing the Atahas Gene

Cristiane T. Citadin,Mirella P. Santos,Estela R. Andrade,Thaís M. Cipriano,Francisco J. L. Aragão 한국육종학회 2022 Plant Breeding and Biotechnology Vol.10 No.2

The detection of the presence and expression of transgenes in genetically modified plants is a key step in the process ofselecting promising lines. We adapted two methods developed for detecting tolerance to the herbicide imazapyr for selection oftransgenic lines expressing the mutated acetohydroxyacid synthase enzyme (AHAS) from Arabidopsis thaliana (Atahas gene). Thiswas achieved using transgenic events from cotton, cowpea, soybean and common bean, which have previously been transformed toexpress the mutated Atahas gene. In the first method, a colorimetric assay was developed that detects acetoin, an intermediate in thebiosynthetic pathway of branched chain amino acids, which is accumulated in the presence of cyclopropanedicarboxylic acid (CPCA),an inhibitor of ketoacid reductoisomerase (KARI). In the presence of the herbicide, it was possible to distinguish non-transgenic fromtransgenic plants. Qualitative analysis of acetoin formed during the AHAS inhibition allowed to indirectly determine the Atahastransgene expression. The second method measured the kinetics of chlorophyll fluorescence emission. Leaf discs pre-treated withimazapyr for 24 hours were evaluated using the modulated fluorimeter for maximum quantum efficiency of Photosystem II (PSII)(Fv/Fm) and relative electron transport rate (ETR). Results showed that almost all species analyzed presented a marked decrease inFv/Fm after treatment with imazapyr. In addition, the ETR was significantly reduced in transgenic plants treated with the herbicide. Collectively, our results showed that it is possible to identify transgenic plants expressing Atahas gene and infer their levels of toleranceto imazapyr at a very early stage after transformation.

Investigation of alpha band of the electroencephalogram before and after a task of proprioceptive neuromuscular facilitation

Luan Correia Costa,Alzira Andrade,Lysnara Lial,Rayele Moreira,Ane Caroline Lima,Astrogildo Magvinier,Ricardo Lira,Anderson Aragão,Pedro Henrique Ulisses,Eric Crespo,Marco Orsini,Silmar Teixeira,Victor 한국운동재활학회 2017 JER Vol.13 No.4

Are home-based exercises effective to reduce blood pressure in hypertensive adults? A systematic review

Gabriel Dias Rodrigues,Ligia Soares Lima,Nicole Cristine Simões da Silva,Paula Gomes Lopes Telles,Teresa Mell da Mota Silva Rocha,Victor Quintella de Aragão Porto,Viviane Veloso Cardoso,Pedro Paulo da 대한고혈압학회 2022 Clinical Hypertension Vol.28 No.-

Sedentarism and chronic non-communicable diseases have been a worldwide health problem that is drastically exacerbated by the COVID-19 pandemic social impacts. Home-based exercises are widely encouraged during social isolation to counterbalance the physical inactive impacts. Although, in the context of hypertension, are home-based exercises effective in blood pressure controlling? Our objective is to conduct a systematic review of high-quality controlled trials comparing the possible effects of different types of home-based exercises in hypertensive patients. The literature search was carried out in three scientific databases: Medline, Europe PMC, and Lilacs. Articles were included following three criteria: analyzing the effect of home-based exercise programs on blood pressure in treated and untreated hypertensive patients; exercises must perform at home and on the frequency, intensity, time, and type (FITT) principle, and the articles were published in English. From the qualitative analysis of 27 original trials screened through 451 identified studies, the main results are the following: 1) both endurance, isometric strength, and respiratory home-based exercise programs were efficient to decrease blood pressure in hypertensive patients; 2) differences in methodological approaches regarding FITT components, distinct blood pressure values at baseline and specific underlying mechanisms must be considered as a potential bias of each home-based interventions. In conclusion, endurance, isometric strength, and breathing home-based programs seems to be effective to reduce blood pressure in hypertensive patients. However, further randomized controlled trials and mechanistic studies must be performing to guide evidence-based recommendations of home-based exercises as antihypertensive therapy.

Is dentin biomodification with collagen cross-linking agents effective for improving dentin adhesion? A systematic review and meta-analysis

Silva Julianne Coelho,Cetira Filho Edson Luiz,Silva Paulo Goberlânio de Barros,Costa Fábio Wildson Gurgel,Saboia Vicente de Paulo Aragão 대한치과보존학회 2022 Restorative Dentistry & Endodontics Vol.47 No.2

Objectives The aim of this investigation was to evaluate the effectiveness of collagen cross-linking agents (CCLAs) used in combination with the adhesive technique in restorative procedures. Materials and Methods In this systematic review, the authors followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses checklist. An electronic search was performed using PubMed, Scopus, Web of Science, Cochrane Library, LILACS, and DOSS, up to October 2020. The gray literature was also researched. Only randomized clinical trials were selected. Results The selection process yielded 3 studies from the 838 retrieved. The addition of CCLAs in the retention of restorations increased the number of events. The postoperative sensitivity scores and marginal adaptation scores showed no significant difference between the CCLA and control groups, and the marginal pigmentation scores showed a significant increase in the CCLA group. There were no caries events in any group throughout the evaluation period. Conclusions This systematic review showed that there is no clinical efficacy to justify the use of CCLAs in the protocols performed. Objectives The aim of this investigation was to evaluate the effectiveness of collagen cross-linking agents (CCLAs) used in combination with the adhesive technique in restorative procedures. Materials and Methods In this systematic review, the authors followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses checklist. An electronic search was performed using PubMed, Scopus, Web of Science, Cochrane Library, LILACS, and DOSS, up to October 2020. The gray literature was also researched. Only randomized clinical trials were selected. Results The selection process yielded 3 studies from the 838 retrieved. The addition of CCLAs in the retention of restorations increased the number of events. The postoperative sensitivity scores and marginal adaptation scores showed no significant difference between the CCLA and control groups, and the marginal pigmentation scores showed a significant increase in the CCLA group. There were no caries events in any group throughout the evaluation period. Conclusions This systematic review showed that there is no clinical efficacy to justify the use of CCLAs in the protocols performed.

Morphometric study on the infraorbital foramen in relation to sex and side of the cranium in northeastern Brazil

Laí,s Carolina Santos Cisneiros de Oliveira,Maria Paula Mendonç,a Silveira,Erasmo de Almeida Jú,nior,Francisco Prado Reis,José,Aderval Aragã,o 대한해부학회 2016 Anatomy & Cell Biology Vol.49 No.1

Detailed knowledge of the possible anatomical and morphometric variations of the infraorbital foramen (IOF) is important for ensuring safe and successful regional anesthesia, and for avoiding iatrogenic nerve injuries during surgery on the middle third of the face. To conduct a morphometric study on the IOF, correlating this with sex and side of the cranium. Two hundred forty-two crania were used (148 male and 94 female). Measurements were made with the aid of digital calipers with precision to 0.01 mm. Presence of foramina and their multiplicity was also observed. The data were analyzed descriptively and analytically. Statistical significance was stipulated as 5% (P≤0.05). The IOF was found bilaterally in all the crania, and 26 of them presented multiplicity. The distance from the IOF to the anterior nasal spine was greater in males on both sides (P<0.001). Statistical differences between the sexes were also seen in relation to the following morphometric variables: height of the left IOF (P=0.007), width of the right IOF (P=0.004), and width of the left IOF (P=0.008), and the measurements were also larger among males. The IOF was present in all the crania and on both sides. It was morphometrically larger in males, on both sides.