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Presence of Proboscipedia and Caudal Gene Homologues in a Bivalve Mollusc
Carpintero, Pablo,Pazos, Antonio Juan,Abad, Marcelina,Sa´nchez, Jose´ Luis,Pe´rez-Paralle´, Maria´ de la Luz 한국생화학분자생물학회 (구 한국생화학회) 2004 BMB Reports Vol.37 No.5
Homeobox genes encode a family of transcription factors that have essential roles in regulating the development of eukaryotes. Although they have been extensively studied in different phyla, relatively little is known about homeoboxcontaining genes and their function in molluscs. In this study, we used a polymerase chain reaction to investigate homeobox genes in the bivalve mollusc Pecten maximus. Four different homeobox sequences were identified; two were homologues of the non-Hox cluster gene caudal and the two remaining sequences had a significant homology to the ANT -C gene proboscipedia. These sequences represent the first cad and pb homologues isolated from a member of the class Bivalvia, phylum Mollusca.
Presence of Proboscipedia and Caudal Gene Homologues in a Bivalve Mollusc
Carpintero, Pablo,Pazos, Antonio Juan,Abad, Marcelina,Sanchez, Jose Luis,De La Luz Perez-Paralle, Maria Korean Society for Biochemistry and Molecular Biol 2004 Journal of biochemistry and molecular biology Vol.37 No.5
Homeobox genes encode a family of transcription factors that have essential roles in regulating the development of eukaryotes. Although they have been extensively studied in different phyla, relatively little is known about homeobox-containing genes and their function in molluscs. In this study, we used a polymerase chain reaction to investigate homeobox genes in the bivalve mollusc Pecten maximus. Four different homeobox sequences were identified; two were homologues of the non-Hox cluster gene caudal and the two remaining sequences had a significant homology to the ANT-C gene proboscipedia. These sequences represent the first cad and pb homologues isolated from a member of the class Bivalvia, phylum Mollusca.
Ángel Aragón,María Cebro-Márquez,Eliseo Perez,Antonio Pazos,Ricardo Lage,José Ramón González-Juanatey,Isabel Moscoso,Carmen Bao-Varela,Daniel Nieto 한국생체재료학회 2020 생체재료학회지 Vol.24 No.3
Background: Cardio myoblast generation from conventional approaches is laborious and time-consuming. We present a bioelectronics on-a-chip for stimulating cells cardio myoblast proliferation during culture. Method: The bioelectronics chip fabrication methodology involves two different process. In the first step, an aluminum layer of 200 nm is deposited over a soda-lime glass substrate using physical vapor deposition and selectively removed using a Q-switched Nd:YVO4 laser to create the electric tracks. To perform the experiments, we developed a biochip composed of a cell culture chamber fabricated with polydimethylsiloxane (PDMS) with a glass coverslip or a cell culture dish placed over the electric circuit tracks. By using such a glass cover slip or cell culture dish we avoid any toxic reactions caused by electrodes in the culture or may be degraded by electrochemical reactions with the cell medium, which is crucial to determine the effective cell-device coupling. Results: The chip was used to study the effect of electric field stimulation of Rat ventricular cardiomyoblasts cells (H9c2). Results shows a remarkable increase in the number of H9c2 cells for the stimulated samples, where after 72 h the cell density double the cell density of control samples. Conclusions: Cell proliferation of Rat ventricular cardiomyoblasts cells (H9c2) using the bioelectronics-on-a-chip was enhanced upon the electrical stimulation. The dependence on the geometrical characteristics of the electric circuit on the peak value and homogeneity of the electric field generated are analyzed and proper parameters to ensure a homogeneous electric field at the cell culture chamber are obtained. It can also be observed a high dependence of the electric field on the geometry of the electrostimulator circuit tracks and envisage the potential applications on electrophysiology studies, monitoring and modulate cellular behavior through the application of electric fields.