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      • Dopamine-functionalized InP/ZnS quantum dots as fluorescence probes for the detection of adenosine in microfluidic chip

        Ankireddy, Seshadri Reddy,Kim, Jongsung Dove Medical Press 2015 INTERNATIONAL JOURNAL OF NANOMEDICINE Vol.10 No.specal

        <P>Microbeads are frequently used as solid supports for biomolecules such as proteins and nucleic acids in heterogeneous microfluidic assays. Chip-based, quantum dot (QD)-bead-biomolecule probes have been used for the detection of various types of DNA. In this study, we developed dopamine (DA)-functionalized InP/ZnS QDs (QDs-DA) as fluorescence probes for the detection of adenosine in microfluidic chips. The photoluminescence (PL) intensity of the QDs-DA is quenched by Zn<SUP>2+</SUP> because of the strong coordination interactions. In the presence of adenosine, Zn<SUP>2+</SUP> cations preferentially bind to adenosine, and the PL intensity of the QDs-DA is recovered. A polydimethylsiloxane-based microfluidic chip was fabricated, and adenosine detection was confirmed using QDs-DA probes.</P>

      • Selective detection of dopamine in the presence of ascorbic acid via fluorescence quenching of InP/ZnS quantum dots

        Ankireddy, Seshadri Reddy,Kim, Jongsung Dove Medical Press 2015 INTERNATIONAL JOURNAL OF NANOMEDICINE Vol.10 No.specal

        <P>Dopamine is a neurotransmitter of the catecholamine family and has many important roles, especially in human brain. Several diseases of the nervous system, such as Parkinson’s disease, attention deficit hyperactivity disorder, restless legs syndrome, are believed to be related to deficiency of dopamine. Several studies have been performed to detect dopamine by using electrochemical analysis. In this study, quantum dots (QDs) were used as sensing media for the detection of dopamine. The surface of the QDs was modified with <SMALL>L</SMALL>-cysteine by coupling reaction to increase the selectivity of dopamine. The fluorescence of cysteine-capped indium phosphide/zinc sulfide QDs was quenched by dopamine with various concentrations in the presence of ascorbic acid. This method shows good selectivity for dopamine detection, and the detection limit was 5 nM.</P>

      • Bio-inspired self-propelled diatom micromotor by catalytic decomposition of H<sub>2</sub>O<sub>2</sub> under low fuel concentration

        Panda, Atanu,Reddy, Ankireddy Seshadri,Venkateswarlu, Sada,Yoon, Minyoung The Royal Society of Chemistry 2018 Nanoscale Vol.10 No.34

        <P>Recently, active bubble-propelled micromotors have attracted great attention for fuel applications. However, for generating bubble-propelled micromotors, additional catalysts, such as Pt, Ag, and Ru, are required. These catalysts are expensive, toxic, and highly unstable for broad applications. To overcome these issues, in this study, we present an innovative methodology for the preparation of self-propelled motor machines using naturally occurring diatom frustules. This natural diatom motor shows effective motion in the presence of a very low concentration (0.8%) of H2O2 as a fuel at pH 7. Due to the unique 3D anisotropic shape of the diatom, the self-propelled motor exhibited unidirectional motion with a speed of 50 μm s<SUP>−1</SUP> and followed pseudo first-order kinetics. It was found that a trace amount of iron oxide (Fe2O3) in the diatom was converted into Fe3O4, which can act as a catalyst to achieve the facile decomposition of H2O2. Interestingly, “braking” of the unidirectional motion was observed upon treatment with EDTA, which blocked the catalytically active site. These results illustrate that diatom catalytic micromotors have opened a new era in the field of catalysis and bioengineering applications.</P>

      • Bio-inspired fluorescence probe for ultra level detection of aliphatic amines

        Panda, Atanu,Venkateswarlu, Sada,Reddy, Ankireddy Seshadri,Yoon, Minyoung Elsevier 2018 Dyes and pigments Vol.156 No.-

        <P><B>Abstract</B></P> <P>The detection of trace amounts of amines is essential to mitigate toxicity and health risks. Various chemical probes have been reported for the detection of amines, which are expensive, difficult to handle, and toxic. In this study, we have developed a bio-inspired fluorescent probe for ultra-level detection of amines in liquid solution. Diatoms, which are silicified algae were used strategically for the detection of aliphatic amines. The presence of an electron-deficient moiety in diatoms improves the ability of diatoms to detect electron-rich organic amines. When an organic amine binds to the pyrolyzed diatom, enhanced photoluminescence and a color change under UV irradiation were observed. Interestingly, fluorescence analysis revealed that the diatoms can selectively detect aliphatic amines with sensitivity up to the pM level, even in the presence of other volatile organic compounds. Among the given analytes, only the electron-rich aliphatic amines significantly enhanced the fluorescence intensity. In contrast, aromatic amines and other organic solvents did not exhibit a fluorescence signal for detection. In particular, ethylenediamine (EDA) contributes the most apparent enhancement effect, with a detection limit of 116 pM. Therefore, diatoms were proved it potential as a tool for monitoring toxic organic molecules.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Developed a bio-inspired 'turn-on' fluorescence probe, a pyrolyzed diatom, for the detection of aliphatic amines in the liquid phase. </LI> <LI> The diatom has excellent selectivity towards aliphatic amines better than aromatic amines. </LI> <LI> The sensitivity of the probe is achieved up to pM level of aliphatic amines. </LI> <LI> Facile identification of the sensing properties by visual color analysis. </LI> <LI> This low-cost material could be an attractive fluorescent probe for the detection of electron-rich molecules. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>Turn-on diatom fluorescence towards aliphatic amine binding.</P> <P>[DISPLAY OMISSION]</P>

      • Fungus-derived photoluminescent carbon nanodots for ultrasensitive detection of Hg<sup>2+</sup> ions and photoinduced bactericidal activity

        Venkateswarlu, Sada,Viswanath, Buddolla,Reddy, Ankireddy Seshadri,Yoon, Minyoung Elsevier 2018 Sensors and actuators. B, Chemical Vol.258 No.-

        <P><B>Abstract</B></P> <P>Herein, we present a sustainable solvent-free synthetic procedure to produce carbon nanodots from common edible mushrooms (<I>Pleurotus</I> spp.). The resulting mushroom carbon nanodots (MCDs) exhibit stable blue fluorescence with high quantum yield (25%). The MCDs are highly dispersible in water because of the enormous number of oxygen- and nitrogen-containing functional groups on the surface. The MCDs can be used as an effective fluorescent probe for label-free detection of Hg<SUP>2+</SUP> ions (detection limit: 4.13nM). To improve the sensitivity, dihydrolipoic acid was attached to the surface of MCDs, resulting in ultra-sensitivity in Hg<SUP>2+</SUP> ion sensing, with a detection limit as low as 17.4 pM. In addition, the MCDs can be used for the labeling of bacteria and as a photoinduced bactericidal agent. Light irradiation of <I>E. coli</I> treated with MCDs showed excellent bactericidal activity relative to the control. These sustainable and affordable carbon materials are potentially compatible for monitoring toxic metals and as a potent visible-light-responsive bactericidal probe.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A facile chemical free synthesis of carbon nanodots using mushrooms </LI> <LI> Temperature dependent synthesis study for high yield of carbon nanodots </LI> <LI> Very stable MCDs with easy functionalization with DHLA under benign conditions </LI> <LI> Ultrahigh sensitive detection of Hg<SUP>2+</SUP> ions with a detection limit as low as 17.4 pM </LI> <LI> Excellent photo-induced reactive oxygen species generation and bactericidal activity </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

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