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- 저자 : Alejandra Rubio (검색결과 3 건)
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Field efficacy of triflumuron against Aedes and Culex mosquitoes in temperate Argentina
Alejandra Rubio,María V. Cardo,Melania T. Junges,Aníbal E. Carbajo,Darío Vezzani 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.1
Aedes aegypti and Culex pipiens s.l. (Linnaeus, 1762 and 1758, respectively) (Diptera: Culicidae) are importantvectors of diseases to humans and a growing public health concern. In order to contribute to the control ofmosquito vectors by low environmental impact approaches we assessed the susceptibility of natural populationsof container-breeding mosquitoes to triflumuron, an insect growth regulator, in temperate Argentina. A field trialwas conducted to evaluate the efficacy of two doses (0.5 ppm and 1 ppm) of triflumuron (SC 48%) againstnatural populations of Ae. aegypti and Culex spp. immatures in flower vases of four cemeteries. The resultsdemonstrated the susceptibility of both target mosquitoes to triflumuron in field conditions. For Ae. aegypti,dose-dependent reductions were achieved in the presence of pupae and the percentage of water-holding containersharbouring L3–4 and/or pupae, whereas the larvae abundance was equally reduced for both doses. ForCulex spp., similar levels of reduction of larvae abundance and pupae presence were achieved with both doses. Significant effects on the response variables measured were recorded up to six to eight weeks post-intervention. Bimonthly applying 1 ppm triflumuron in the context of an integrated mosquito management should achieve alasting control of Ae. aegypti and Culex spp. in small artificial containers with minimal environmental impacts.
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Alejandra Bermudez-Oria,Guillermo Rodrıguez-Gutierrez,Fatima Rubio-Senent,Marta Sanchez-Carbayo,Juan Fernandez-Bolanos 한국식품영양과학회 2020 Journal of medicinal food Vol.23 No.7
Bladder cancer (BC) is one of the most common human cancers. There is an interest in controlling and treating BC and other types of cancer via the use of natural substances and/or combination chemotherapy. Modified forms of pectin have been reported to possess anticancer bioactivity related to the interaction of galactosyl, a main component of pectin, with galectin-3, a carbohydrate-binding protein that is overexpressed on many types of cancer cells. In this study, the antiproliferative effect on BC of novel modified pectins extracted from olives was evaluated. Pectoliv extracts, with high polyphenol content associated to polysaccharides rich in pectin, exhibited an important antiproliferative capacity in vitro against four human BC cells lines, RT112, T24, J82, and SCaBER. Pectoliv treatment reduced the expression of galectin-1 and galectin-3 and significantly inhibited the agglutination of erythrocytes. Thus, Pectoliv may have the potential for development as a novel galectin-3 inhibitor.
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( Viviana P. Rubio ),( Alejandra Bravo ),( Jorge Olmos ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.1
In this work, we isolated a surface layer protein (SLP) from a Bacillus thuringiensis (Bt) strain to evaluate it cytotoxic effects against MDA-MB-231 human breast cancer cells. AP11 was selected from a g roup o f B t s trains u sing SLP o lig onucleotides developed f rom Bacillus conserved regions. The AP11 strain was grown in Luria Bertani medium until the late exponential phase; an 86 kDa protein was extracted using 5 M LiCl and identified by liquid chromatography- tandem mass spectrometry. It corresponded to a multispecies SLP highly similar to previously described SLPs in Bt. The MDA-MB-231 breast cancer cells LC50 was obtained using 0.25 μg/ml of the isolated SLP. HaCat non-cancerous cells presented 90% survival using the same protein concentration. Our data suggest that SLP cytotoxicity against MDA-MB-231 could be induced by an interaction with the CDH11 cell membrane receptor.
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