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Al-Husseini, Wijdan,Chen, Yizhou,Gondro, Cedric,Herd, Robert M.,Gibson, John P.,Arthur, Paul F. Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.10
MicroRNAs (miRNAs) are short non-coding RNAs that post-transcriptionally regulate expression of mRNAs in many biological pathways. Liver plays an important role in the feed efficiency of animals and high and low efficient cattle demonstrated different gene expression profiles by microarray. Here we report comprehensive miRNAs profiles by next-gen deep sequencing in Angus cattle divergently selected for residual feed intake (RFI) and identify miRNAs related to feed efficiency in beef cattle. Two microRNA libraries were constructed from pooled RNA extracted from livers of low and high RFI cattle, and sequenced by Illumina genome analyser. In total, 23,628,103 high quality short sequence reads were obtained and more than half of these reads were matched to the bovine genome (UMD 3.1). We identified 305 known bovine miRNAs. Bta-miR-143, bta-miR-30, bta-miR-122, bta-miR-378, and bta-let-7 were the top five most abundant miRNAs families expressed in liver, representing more than 63% of expressed miRNAs. We also identified 52 homologous miRNAs and 10 novel putative bovine-specific miRNAs, based on precursor sequence and the secondary structure and utilizing the miRBase (v. 21). We compared the miRNAs profile between high and low RFI animals and ranked the most differentially expressed bovine known miRNAs. Bovine miR-143 was the most abundant miRNA in the bovine liver and comprised 20% of total expressed mapped miRNAs. The most highly expressed miRNA in liver of mice and humans, miR-122, was the third most abundant in our cattle liver samples. We also identified 10 putative novel bovine-specific miRNA candidates. Differentially expressed miRNAs between high and low RFI cattle were identified with 18 miRNAs being up-regulated and 7 other miRNAs down-regulated in low RFI cattle. Our study has identified comprehensive miRNAs expressed in bovine liver. Some of the expressed miRNAs are novel in cattle. The differentially expressed miRNAs between high and low RFI give some insights into liver miRNAs regulating physiological pathways underlying variation in this measure of feed efficiency in bovines.
Finding the Julian Dates of the Islamic Events Before Hijra Using Computer
Al-Dargazelli, Shaikh Muhammad Al-Husseini Jamal N. Hussein Shetha,Fatoohi, Hamid M. An-Ni'aymi Louay J. 한국외국어대학교 중동연구소 1992 중동연구 Vol.11 No.-
A new lunar calendar, called the Muhammadan calendar, starting with the year and month of birth of the Prophet Muhammad was introduced to cover all the history of Islam which began with the birth of the Prophet Muhammad. A lunar historical sequence of the most important Islamic events during the life of Prophet Muhammad was obtained by finding the dates of these events according to the Muhammadan calendar. After a profound study for the historical sources, the birth year of the Prophet Muhammad was considered to be 570 A.D. A computer program for determining the time and exact Julian date of the birth of new moons was used in addition to a manual method to determine the beginning of every Muhammadan month under consideration. The Mutammadan dates were converted into Julian dates. The exact Julian dates of the Islamic events before Hijra (migration) were found for the first time, as well as, the most accurate Julian dates of the Islamic events after Hijra. The results revealed that the most important Islamic events had occurred on Fridays, explaining why Friday is considered by Islam to be a sacred day.