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- 저자 : Aihua Song (검색결과 6 건)
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Liping Song,Xia Li,Feng Zu,Changbin Gao,Bincai Wang,Chufa Lin,Jinxing Tu,Aihua Wang,Guolin Zhou 한국유전학회 2019 Genes & Genomics Vol.41 No.12
Background WS24-3A is a newly bred non-heading Chinese cabbage genic male-sterile line, in which sterility is controlled by a recessive gene, designated as Bra2ms. WS24-3A has been used for hybrid breeding. Objective To reveal the underlying molecular mechanisms responsible for the sterility of WS24-3A. Methods Cytological observation of the process of sterile/fertile anther development was performed to determine the tissue and stage in which sterility occurs. Phenotyping and transcriptomic analyses were performed to identify differentially expressed genes (DEGs) between sterile and fertile flower buds at different stages. Results Cytological analysis revealed no tetrads at stage 7 or at later stages of anther development, and the degradation of callose was delayed. Abnormal meiocytes were surrounded by sustaining callose that degenerated gradually in WS24-3A. Comparative transcript profiling identified 3282 DEGs during three anther developmental stages, namely, pre-meiotic anther, meiotic anther, and anthers with single-celled pollen stage. The difference in DEG percentage between up-regulated and down-regulated at meiotic anther stage was obviously larger than at the other two stages; further, most DEGs are important for male meiosis, callose synthesis and dissolution, and tapetum development. Ten DEGs were found to be involved in anther and pollen development, which were analyzed by quantitative PCR. Conclusion Bra2ms affected gene expression in meiocytes and associated with callose synthesis, degradation and tapetum development. Our results provide clues to elucidate the molecular mechanism of genic male sterility in non-heading Chinese cabbage.
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Candidate Genes with Ovulation by Differential Display PCR in Small Tail Han Sheep
Liu, Shufang,Li, Hongbin,Song, Xuemei,Wang, Aihua,Wei, Caihong,Du, Lixin Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.9
To find the candidate genes concerned with ovulation rate of sheep, Differential Display Reverse Transcription Polymerase Chain Reaction was employed to find the differently expressed cDNA controlling ovulation in the Small Tail Han sheep of polyembryony and in Tan sheep of single birth. Twenty-four primer pairs of three anchored primers and eight arbitrary primers were assembled to amplify the specialized bands from these sheep. Positive cross tests were applied to optimize the ascertainable PCR conditions in which different special bands can be identified by silver strain in one PCR tube. After eliminating the false positive PCR products by Northern hybridization, 24 differential display bands were acquired from the ovary in the Small Tail Han sheep. These EST bands were sequenced and 18 different ESTs were found in which five ESTs had several copies and 13 ESTs had only one copy. Comparing these ESTs with homologous sequences by BLAST in the GenBank, there were six ESTs with known open reading frame (ORF) and function, three ESTs with known ORF and no function, and 9 ESTs without homologous sequence. These ESTs partly represent several genes such as NOS2, tensin, TCRA, CDKN1A, ESR1 and ACTB which express especially in Small Tail Han sheep.
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Functional dichotomy between OX40 and 4-1BB in modulating effector CD8 T cell responses.
Lee, Seung-Woo,Park, Yunji,Song, Aihua,Cheroutre, Hilde,Kwon, Byoung S,Croft, Michael American Association of Immunologists 2006 Journal of Immunology Vol.177 No.7
<P>Members of the TNFR family are thought to deliver costimulatory signals to T cells and modulate their function and survival. In this study, we compare the role of two closely related TNFR family molecules, OX40 and 4-1BB, in generating effector CD8 T cells to Ag delivered by adenovirus. OX40 and 4-1BB were both induced on responding naive CD8 T cells, but 4-1BB exhibited faster and more sustained kinetics than OX40. OX40-deficient CD8 T cells initially expanded normally; however, their accumulation and survival at late times in the primary response was significantly impaired. In contrast, 4-1BB-deficient CD8 T cells displayed hyperresponsiveness, expanding more than wild-type cells. The 4-1BB-deficient CD8 T cells also showed enhanced maturation attributes, whereas OX40-deficient CD8 T cells had multiple defects in the expression of effector cell surface markers, the synthesis of cytokines, and in cytotoxic activity. These results suggest that, in contrast to current ideas, OX40 and 4-1BB can have a clear functional dichotomy in modulating effector CD8 T cell responses. OX40 can positively regulate effector function and late accumulation/survival, whereas 4-1BB can initially operate in a negative manner to limit primary CD8 responses.</P>
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Toward Activity Mapping for Artifact-Centric Business Process
Yuyu Yin,Zhengshuang Zhu,Min Gao,Aihua Song 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.6
The existing method of task mapping between process models is mostly based on the similarity of labels to get the similarity between tasks, which is affected by a single factor and easy to cause errors. This paper proposes a task mapping method for Artifact-Centric business process analyzes the data operation in business process execution. At first we add the description of data operation to EZ-Flow model. Secondly select the similar artifact attributes of the two business artifact by whole artifacts similarity calculation and based them to calculate two tasks’ similarity by label similarity, artifact operation similarity and context similarity calculations. Finally, the task mapping comes from the optimal selection of tasks’ similarities. Experimental results verify the effectiveness of the method, and show that the method reflects the data operation characteristics of business process model, as artifact task mapping of the process model provides a feasible method.
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<i>MIF</i> allele-dependent regulation of the MIF coreceptor CD44 and role in rheumatoid arthritis
Yoo, Seung-Ah,Leng, Lin,Kim, Bum-Joon,Du, Xin,Tilstam, Pathricia V.,Kim, Kyung Hee,Kong, Jin-Sun,Yoon, Hyung-Ju,Liu, Aihua,Wang, Tian,Song, Yan,Sauler, Maor,Bernhagen, Jurgen,Ritchlin, Christopher T. National Academy of Sciences 2016 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.113 No.49
<P>Fibroblast-like synoviocytes mediate joint destruction in rheumatoid arthritis and exhibit sustained proinflammatory and invasive properties. CD44 is a polymorphic transmembrane protein with defined roles inmatrix interaction and tumor invasion that is also a signaling coreceptor for macrophage migration inhibitory factor (MIF), which engages cell surface CD74. High-expression MIF alleles (rs5844572) are associated with rheumatoid joint erosion, but whether MIF signaling through the CD74/CD44 receptor complex promotes upstream autoimmune responses or contributes directly to synovial joint destruction is unknown. We report here the functional regulation of CD44 by an autocrine pathway in synovial fibroblasts that is driven by high-expression MIF alleles to up-regulate an inflammatory and invasive phenotype. MIF increases CD44 expression, promotes its recruitment into a functional signal transduction complex, and stimulates alternative exon splicing, leading to expression of the CD44v3-v6 isoforms associated with oncogenic invasion. CD44 recruitment into the MIF receptor complex, downstream MAPK and RhoA signaling, and invasive phenotype require MIF and CD74 and are reduced by MIF pathway antagonists. These data support a functional role for high-MIF expression alleles and the two-component CD74/CD44 MIF receptor in rheumatoid arthritis and suggest that pharmacologic inhibition of this pathway may offer a specific means to interfere with progressive joint destruction.</P>
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