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- 저자 : Abdelhafidh Dhouib (검색결과 2 건)
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Rim Khlifi-Slama,Tahar Mechichi,Sami Sayadi,Abdelhafidh Dhouib 한국미생물학회 2012 The journal of microbiology Vol.50 No.2
The purpose of the present study was to determine the effect of natural mediators on the stability of the Trametes trogii crude laccase in the process of decolourization of textile effluents. Acetosyringone allowed the highest wastewaters decolourization rate of 25%. At higher concentrations of acetosyringone, the relative activity of laccase decreased approximately by between 38% and 88% after 5 days of incubation. T. trogii laccase was strongly inactivated at 3mM syringaldehyde, after 3 days of incubation. However,laccase activity is more stable in the presence of the vanillin and m-coumarate. The T. trogii growth on solid effluentbased-medium was examined and evaluated by measuring the colony diameter in cm. T. trogii was completely inhibited on 100:0 and 80:20 effluent:water solid medium, however,colony diameter reached 5 cm on 60:40 effluent:water solid medium after 13–14 days incubation. When the textile effluent was pre-treated with laccase and laccase-acetosyringone system, the colony diameter of 2 cm of T. trogii on 80:20effluent:water solid medium was reached after 14 and 10days of incubation respectively.
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Bassem Jaouadi,Semia Ellouz-Chaabouni,Mamdouh Ben Ali,Ezzedine Ben Messaoud,Belgacem Naili,Abdelhafidh Dhouib,Samir Bejar 한국생물공학회 2009 Biotechnology and Bioprocess Engineering Vol.14 No.4
The newly Tunisian soil-isolated bacterium, producing the alkaline proteinase termed SAPB that was already purified and characterized [1], was assigned as Bacillus pumilus CBS strain on the basis of biochemical properties and 16S rRNA gene sequencing. The maximum protease activity recorded after 24 h of incubation in an optimized medium at 37°C was 6,500 U/mL in shaking flask culture and 25,000 U/mL in fermentor. SAPB showed excellent stability and compatibility in laundry detergent retaining more than 98% of its initial activity after pre-incubation for 1 h at 40°C with Det, followed by OMO (97%), Dinol (94%), and Dixan (93%). Examination of various stained cloth pieces exhibited a remarkable efficiency in the removal of blood and chocolate stains. More interestingly, SAPB demonstrated powerful dehairing capabilities of hair removal from skin with minimal damage on the collagen and a nearly complete feather-degradation. Likewise, Bacillus pumilus CBS effectively degraded feather-meal (98.5%), chicken feather (92%), goat hair (80%), and bovine hair (68%) whereas sheep wool under went less degradation. Keratin-degradation resulted in sulfhdryl group formation (0.95~3.91 μM).
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