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- 저자 : A Michalopoulos (검색결과 4 건)
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Rupture of Right Hepatic Duct into Hydatid Cyst
Nickolaos Michalopoulos,Styliani Laskou,Theodossis S. Papavramidis,Ioannis Pliakos,Eustathios Kotidis,Isaak Kesisoglou,Spiros T. Papavramidis 대한의학회 2012 Journal of Korean medical science Vol.27 No.8
Echinococcal disease can develop anywhere in the human body. The liver represents its most frequent location. Hepatic hydatid cysts may rupture into the biliary tract, thorax,peritoneum, viscera, digestive tract or skin. We report a rare case with rupture of the right hepatic duct into a hydatid cyst in a woman with known hydatid disease and choledocholithiasis. The increased intra-luminal pressure in the biliary tree caused the rupture into the adjacent hydatid cyst. The creation of the fistula between the right hepatic duct and the hydatid cyst decompressed the biliary tree, decreased the bilirubin levels and offered a temporary resolution of the obstructive jaundice. Rupture of a hydatid cyst into the biliary tree usually leads to biliary colic, cholangitis and jaundice. However, in case of obstructive jaundice due to choledocholithiasis, it is possible that the cyst may rupture by other way around while offering the patient a temporary relief from his symptoms.
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TS Papavramidis,S Potsi,D Paramythiotis,A Michalopoulos,VN Papadopoulos,V Douros,A Pantoleon,A Foutzila-Kalogera,I Ekonomou,N Harlaftis 대한의학회 2009 Journal of Korean medical science Vol.24 No.6
The present case is one of gallstone obstructive ileus due to gallstones 3 yr after laparoscopic cholecystectomy. It is interesting because of the sex of the patient, the fact that ileus occurred 3 yr after cholecystectomy and that the localization of the obstruction was an old side-to-side ileoileal anastomosis due to a diverticulectomy following intussusception of Meckels’ diverticulum at the age of 3.
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Optimizing Decellularization Strategies for the Efficient Production of Whole Rat Kidney Scaffolds
Mallis Panagiotis,Oikonomidis Charalampos,Dimou Zetta,Stavropoulos-Giokas Catherine,Michalopoulos Efstathios,Katsimpoulas Michalis 한국조직공학과 재생의학회 2021 조직공학과 재생의학 Vol.18 No.4
BACKGROUND: Renal dysfunction remains a global issue, with chronic kidney disease being the 18th most leading cause of death, worldwide. The increased demands in kidney transplants, led the scientific society to seek alternative strategies, utilizing mostly the tissue engineering approaches. Unlike to perfusion decellularization of kidneys, we proposed alternative decellularization strategies to obtain acellular kidney scaffolds. The aim of this study was the evaluation of two different decellularization approaches for producing kidney bioscaffolds. METHODS: Rat kidneys from Wistar rats, were submitted to decellularization, followed two different strategies. The decellularization solutions used in both approaches were the same and involved the use of 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate and sodium dodecyl sulfate buffers for 12 h each, followed by incubation in a serum medium. Both approaches involved 3 decellularization cycles. Histological analysis, biochemical and DNA quantification were performed. Cytotoxicity assay and repopulation of acellular kidneys were also applied. RESULTS: Histological, biochemical and DNA quantification confirmed that the 2nd approach had the best outcome regarding the kidney composition and cell elimination. Acellular kidneys from both approaches were successfully recellularized. CONCLUSION: Based on the above data, the production of kidney scaffolds with the proposed cost- effective decellularization approaches, was efficient. BACKGROUND: Renal dysfunction remains a global issue, with chronic kidney disease being the 18th most leading cause of death, worldwide. The increased demands in kidney transplants, led the scientific society to seek alternative strategies, utilizing mostly the tissue engineering approaches. Unlike to perfusion decellularization of kidneys, we proposed alternative decellularization strategies to obtain acellular kidney scaffolds. The aim of this study was the evaluation of two different decellularization approaches for producing kidney bioscaffolds. METHODS: Rat kidneys from Wistar rats, were submitted to decellularization, followed two different strategies. The decellularization solutions used in both approaches were the same and involved the use of 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate and sodium dodecyl sulfate buffers for 12 h each, followed by incubation in a serum medium. Both approaches involved 3 decellularization cycles. Histological analysis, biochemical and DNA quantification were performed. Cytotoxicity assay and repopulation of acellular kidneys were also applied. RESULTS: Histological, biochemical and DNA quantification confirmed that the 2nd approach had the best outcome regarding the kidney composition and cell elimination. Acellular kidneys from both approaches were successfully recellularized. CONCLUSION: Based on the above data, the production of kidney scaffolds with the proposed cost- effective decellularization approaches, was efficient.
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Vitrified Human Umbilical Arteries as Potential Grafts for Vascular Tissue Engineering
Mallis Panagiotis,Katsimpoulas Michalis,Kostakis Alkiviadis,Dipresa Daniele,Korossis Sotiris,Papapanagiotou Aggeliki,Kassi Eva,Stavropoulos-Giokas Catherine,Michalopoulos Efstathios 한국조직공학과 재생의학회 2020 조직공학과 재생의학 Vol.17 No.3
BACKGROUND The development of a biological based small diameter vascular graft (d\6 mm), that can be properly stored over a long time period at - 196 』C, in order to directly be used to the patients, still remains a challenge. In this study the decellularized umbilical arteries (UAs) where vitrified, evaluated their composition and implanted to a porcine model, thus serving as vascular graft. METHODS Human UAs were decellularized using 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) and sodium dodecyl sulfate (SDS) detergents. Then, vitrified with vitrification solution 55 (VS55) solution, remained for 6 months in liquid nitrogen and their extracellular matrix composition was compared to conventionally cryopreserved UAs. Additionally, total hydroxyproline, sulphated glycosaminoglycan and DNA content were quantified in all samples. Finally, the vitrified umbilical arteries implanted as common carotid artery interposition graft to a porcine animal model. RESULTS Decellularized and vitrified UAs characterized by proper preservation of extracellular matrix proteins and tissue architecture, whereas conventionally cryopreserved samples exhibited a disorganized structure. Total hydroxyproline content was preserved, although sulphated glycosaminoglycan and DNA contents presented significantly alterations in all samples. Implanted UAs successfully recellularized and remodeled as indicated by the histological analysis. CONCLUSION Decellularized and vitrified UAs retained their structure function properties and can be possible used as an alternative source for readily accessible small diameter vascular grafts.
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