초임계 이산화탄소를 이용한 Chlorella vulgaris의 오일 추출

유종훈(Jong-Hoon Ryu),박미란(Mi-Ran Park),임교빈(Giobin Lim) 한국생물공학회 2011 KSBB Journal Vol.26 No.5

In this study, two different extraction techniques, organic solvent extraction and supercritical carbon dioxide (SCCO₂) extraction, were employed to evaluate the extraction efficiency of oil from Chlorella vulgaris. In the organic solvent extraction, the effects of various organic solvent on the extraction yield were investigated. The SCCO₂ extraction was carried out while varying such operating parameters as temperature, pressure, SCCO₂ flow rate, and cosolvent. About 4.9 wt% of oil was extracted from ground Chrollera vulgaris for 18 h when dichloromethane/methanol (2:1, v/v) was used as an extraction solvent. The oil yield of the SCCO₂ extraction was found to be very low (0.53 wt%) and to increase up to about 0.86 wt% with the addition of cosolvent.

W/O/W 다중유화법을 이용한 젬시타빈 함유 Methoxy Poly(ethylene glycol)-b-Poly(L-lactide) 미립자 제조

유종훈(Jong-Hoon Ryu),정인일(In-Il Jung),이지은(Ji-Eun Lee),임교빈(Giobin Lim) 한국생물공학회 2011 KSBB Journal Vol.26 No.4

In this study, gemcitabine-loaded methoxy poly(ethylene glycol)-b-poly(L-lactide) (MPEG-PLLA) microparticles with different PEG block lengths were prepared by a W/O/W double emulsion technique. The present study focuses on the investigation of the influence of various preparative parameters such as the ratio of internal water phase and oil phase, polymer concentration, solvent composition of organic phase and salt concentration of external water phase on the morphology and encapsulation efficiency of the microparticles. The microparticles fabricated at high volume ratios of internal water phase to oil phase and at high polymer concentrations showed a relatively high encapsulation efficiency and low porosity. When a dichloromethane/ethyl acetate mixture was used as solvent, both the encapsulation efficiency and drug loading of the microparticles decreased as the level of ethyl acetate increased. The addition of a salt (NaCl) to the external water phase significantly improved the encapsulation efficiency up to 40%, and the microparticles became more spherical with their size and porosity decreased.

초임계 이산화탄소와 유기용매를 이용한 Chlorella vulgaris 오일의 추출

유종훈(Jong-Hoon Ryu),박미란(Mi-Ran Park),임교빈(Giobin Lim) 한국생물공학회 2014 KSBB Journal Vol.29 No.2

Three different types of extraction processes, which used supercritical carbon dioxide (SCCO₂) and organic solvent, were attempted to improve the extraction yield of oil from Chlorella vulgaris: cosolvent-modified SCCO₂ extraction, SCCO₂ extraction with ultrasonic sample treatment in organic solvent, and static extraction with organic solvent followed by dynamic SCCO₂ extraction. Among these, the last SCCO₂ extraction process was found to be most effective in the extraction of oil. Compared with pure SCCO₂ extraction, the extraction yield of oil was observed to increase about 7 times.

초임계 이산화탄소를 이용한 Zein 미립자 제조

유종훈(Jong-Hoon Ryu),임교빈(Giobin Lim) 한국생물공학회 2012 KSBB Journal Vol.27 No.4

In this study, zein microparticles and drug-loaded zein microparticles were prepared using supercritical ASES technique. The effects of operating parameters on particle size and morphology were investigated. ASES-processed zein microparticles consisted of agglomerates of very fine unit particles. As temperature increased, the size of unit particles increased and their morphology became more spherical. The addition of water to the solvents for zein resulted in the formation of more spherical microparticles. The release characteristics of drug-loaded zein microparticles were also studied.

초임계 이산화탄소를 이용한 2-Hydroxypropyl-β-Cyclodextrin 미립자와 이부프로펜과의 포접복합체 제조

유종훈(Jong-Hoon Ryu) 한국청정기술학회 2013 청정기술 Vol.19 No.3

초임계 이산화탄소를 역용매로 이용하는 aerosol solvent extraction system (ASES) 방법을 사용하여 HP-β-CD 미립자를 제조하였으며, 공정변수가 입자의 크기와 형태에 미치는 영향을 조사하였다. 또한, 초임계 이산화탄소를 이용하여 이부프로펜과 HP-β-CD의 포접복합체를 제조하였으며, ASES 공정에 의해 변형된 HP-β-CD의 입자 형상이 포접효율에 미치는 영향에 대해 고찰하였다. ASES 공정으로 제조된 HP-β-CD 미립자는 50~200 nm 크기의 나노 입자들이 응집된 입자 형상을 나타내었다. 에탄올 수용액을 HP-β-CD의 용매로 사용한 경우 구형의 입자가 제조되었으며, 물의 양이 증가함에 따라 입자의 크기가 증가하였다. 초임계 이산화탄소를 이용해 고체상태에서 이부프로펜/HP-β-CD 포접복합체를 제조하는 경우 초임계 ASES 방법에 의한 미세입자화 공정을 통해 포접효율을 향상시킬 수 있는 가능성을 확인하였다. The microparticles of 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) were prepared using aerosol solvent extraction system (ASES) by employing supercritical carbon dioxide as an antisolvent, The effects of various process parameters such as temperature, pressure, solution concentration and solution flow rate on the formation of HP-β-CD microparticles were investigated. The HP-β-CD microparticles prepared by the ASES process were observed to consist of agglomerates of nano-sized (50-200 nm) particles. When an aqueous solution of ethanol was used as a solvent for HP-β-CD, the HP-β-CD particles were found to be spherical in shape and to become larger as the water content increased. It was confirmed that the micronization of HP-β-CD using the ASES process could enhance the inclusion efficiency of ibuprofen/HP-β-CD complexes significantly.

오일/물 에멀젼의 분리막 투과 특성

김종표(Jong-Pyo Kim   ),임진수(Jin-Soo Lim   &),유종훈  (Jong-Hoon Ryu   ),김재진(Jae Jin Kim   &),정건용(Kun Yong Chung),전명석(Myung-Suk Chun  한국청정기술학회 1999 청정기술 Vol.5 No.2

세라믹 정밀여과막의 계면활성제 처리 효과

김종표(Jong Pyo Kim),남현희(Hyun Hee Nam),유종훈(Jong Hoon Ryu),김재진(Jae Jin Kim),배재흠(Jae Heum Bae),김정호(Jeong Ho Kim),권영돈(Young Don Kwon) 한국공업화학회 2001 공업화학 Vol.12 No.5

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초임계 유체를 이용한 젬시타빈 함유 PLLA/젬시타빈-PLLA 미립자의 제조 및 방출 특성

홍진희(Jin-Hee Hong),임교빈(Giobin Lim),유종훈(Jong-Hoon Ryu) 한국생물공학회 2014 KSBB Journal Vol.29 No.6

In this study, gemcitabine (Gem)-Poly(L-lactic acid) (PLLA) conjugates were synthesized through an amide linkage reaction. Then, the microparticles of Gem-PLLA/PLLA blends containing gemcitabine were prepared using a supercritical fluid process, called aerosol solvent extraction system(ASES). Gemcitabine-loaded Gem-PLLA/PLLA microparticles obtained from the ASES process showed a spherical shape. The amount of gemcitabine released after 30 day incubation in a phosphate buffer solution of pH 7.4 was about 90% of the total amount of gemcitabine present in the product.

초음파가 적용된 초임계 유체 공정을 이용한 캐놀라오일 추출

황아름(Ah-Reum Hwang),임교빈(Giobin Lim),유종훈(Jong-Hoon Ryu) 한국생물공학회 2010 KSBB Journal Vol.25 No.5

본 연구에서는 초임계 이산화탄소의 추출기술에 초음파 적용을 위한 기초 연구로서 캐놀라 씨앗의 입자의 크기, CO₂ 유속, 추출기의 종횡비, 초음파 파워 등의 공정변수가 캐놀라 오일의 추출 속도와 수율에 미치는 영향에 대하여 조사하였다. 초임계 이산화탄소 추출에 있어 씨앗을 더 작게 분쇄할수록 더 빠른 추출속도를 보였으며, 추출기의 종횡비가 감소할수록, CO₂의 유속이 증가할수록 추출 속도는 증가하였다. 그러나 초음파가 적용된 초임계 이산화탄소 추출의 경우 0.6 mm 이하로 분쇄된 씨앗의 입자 크기 분포가 가장 큰 시료 C의 경우 입자들의 뭉침 현상 증가로 인해 오히려 초음파의 적용이 추출속도와 수율을 크게 감소시켰으며, 12.0 L/min의 CO₂ 유속에서도 추출 속도와 수율이 감소하는 것을 확인하였다. 초음파의 적용은 초기 추출시간 50-70분동안 추출속도 증가에 거의 영향을 미치지 않았으며, 초음파 파워 또한 추출 속도와 수율의 증가에 큰 영향을 미치지 않음을 확인할 수 있었다. The objective of this study was to investigate the effect of ultrasound on the extraction of oil from canola seeds when supercritical carbon dioxide (SCCO₂) was used as an extraction solvent. The ultrasound-assisted SCCO₂ extraction were carried out while varying such operating parameters as particle size of crushed canola seed, flow rate of SCCO₂, aspect ratio of the extraction vessel, and ultrasound power. The extraction rate decreased with increasing particle size of samples, showing a maximun at a CO₂ flow rate of 6.2 L/min. Both the extraction rate and extraction yield increased with a decrease in the aspect ratio of the extraction vessel. For the ultrasoundassisted SCCO₂ extraction, the extraction yield was slightly increased when the CO₂ flow rate was below 6 mL/min with sample A and B.

초임계 PGSS 공정을 이용한 Coriander 정유 함유 PEG 미세입자 제조

최진아(Jin-Ah Choi),임교빈(Giobin Lim),유종훈(Jong-Hoon Ryu) 한국생물공학회 2010 KSBB Journal Vol.25 No.4

본 연구에서는 coriander 정유의 안정성 향상을 위해 초임계 PGSS 공정을 이용하여 coriander 정유가 봉입된 PEG 미세입자를 제조하였으며 공정 온도와 압력, 노즐 크기가 미세입자의 크기, 형태 및 정유의 봉입률에 미치는 영향에 대하여 조사하였다. 100 μm 노즐을 사용하여 얻어진 미세입자의 형상을 분석한 결과 1-10 μm 크기의 구형 입자 형태를 나타내었으나 노즐의 크기가 증가한 경우에는 덩어리진 입자가 얻어짐을 확인하였다. 온도, 압력 변화에 따라 얻어진 미세입자의 경우 일정 압력 조건에서 공정 온도가 높아짐에 따라, 일정 온도 조건에서 공정 압력이 낮아짐에 따라 구형입자 비율이 증가함을 보였으며, 일정 온도 조건에서는 공정 압력이 높아짐에 따라 더 많은 기공을 가지는 입자가 생성됨을 확인하였다. 제조된 PEG 미세입자 내 coriander 정유의 봉입률은 모든 온도 조건에서 가장 낮은 압력인 75 bar일 때 가장 높은 값을 보였다. In the present study, biocompatible poly (ethylene glycol) (PEG) microparticles containing coriander essential oil were prepared using a supercritical particles from gas saturated solution (PGSS) process to improve the stability of the coriander oil. The effects of various process parameters such as temperature, pressure, and nozzle diameter on the morphology and entrapment efficiency of coriander oil loaded PEG microparticles were then investigated. A positive influence on the formation of spherical microparticles was observed with increasing temperature and decreasing pressure. Furthermore, somewhat more porous microparticles were produced with an increase in pressure. At a given temperature, the highest entrapment efficiency of coriander essential oil in PEG microparticles was observed under the lowest experimental pressure condition.