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참다랑어(Thunnus orientalis) 치어에 있어서 전갱이(Trachrus japonicus) 근육 엑기스 중의 섭이촉진물질 검색
지승철,타카오카오사무,세오카마나부,코오바라준,호소카와히테츠요,시메노사다오,정관식,이시우,타키이켄지,Ji, Seung-Cheol,Takaoka, Osamu,Seoka, Manabu,Kohbara, Jun,Hosokawa, Hidetuyo,Shimeno, Sadao,Jeong, Gwan-Sik,Lee, Si-Woo,Takii, Kenji 한국양식학회 2007 韓國養殖學會誌 Vol.20 No.1
참다랑어(Thunnus orientalis)의 배합사료 개발을 위해 전갱이(Trachrus japonicus) 근육 합성 엑기스(SE)를 사용하여 omission test를 통해 섭이촉진물질을 검색하였다. 인공종묘 생산된 참다랑어 치어(평균체중 $9.0{\pm}0.91\;g$: 실험 1, 2, 3; $1.6{\pm}0.23\;g$: 실험 4)를 사용하여 전갱이 근육 100 g 상당 함량의 각각의 섭이촉진 물질을 카제인 기초 사료에 첨가하여 총 4회의 사육실험을 실시하였다. 실험 1에서 인공합성엑기스(SE)와 SE로부터 아미노산 관련 화합물(SE-A), 핵산관련물질(SE-N) 그리고 유기염기화합물(SE-O)을 각각 제외한 시험액을 제조하여 천연 전갱이 근육 엑기스(NE)와 비교한 결과, SE-A와 SE-N구는 SE보다 낮은 섭이활성을 보였으나, SE-O구는 SE와 유사한 섭이활성을 보였다. 실험 2에서는 IMP가 섭이 촉진에 가장 많은 영향을 미치는 것이 인정되었으며, 실험 3에서는 L-alanine, L-glutamic acid, L-histidine, L-lysine, taurine 그리고 IMP의 혼합물이 SE와 섭이활성의 차이를 보이지 않았다. 실험 4에서는 L-histidine, L-glutamine 그리고 IMP 혼합물이 가장 높은 섭이활성을 보였으며, 그 다음으로는 SE, L-lysine, L-alanine and IMP 혼합물, L-histidine, L-glutamic acid, L-lysine and L-alanine과 IMP의 혼합물이 높은 섭이활성을 보였다. 본 연구결과 참다랑어 치어의 유효 섭이촉진물질은 L-histidine, L-glutamic acid 그리고 IMP로 판단되었다. For establishing a basal diet for the Pacific bluefin tuna Thunnus orientalis (PBT), feeding stimulants were initially identified by omission test using the synthetic extract of horse mackerel, Trachurus japonicus. Four feeding trials were conducted using juvenile PBT weighing $9.0{\pm}0.91\;g$ (trial 1, 2 and 3) and $1.6{\pm}0.23\;g$ (trial 4), which were originated from an artificial seedling production. The fish fed the casein diet with each test solution were added at the ratio of 100 g casein diet to 100 g jack mackerel muscle. A complete synthetic extract of jack mackerel containing all 3 fractions, amino acid, nucleotide and organic nitrogenous base, exhibited a comparable feeding stimulant activity compared to that of natural extract. The omission of nucleotide or amino acid fraction showed lower feeding activity, but the omission of other nitrogenous fraction maintained a similar feeding stimulant activity compared to that of the synthetic extract (trial 1). Inosine-5' monophosphate $Na_2$ (IMP) was identified as a major constituent for maintaining feeding activity. The mixture of L-alanine, L-glutamic acid, L-histidine, L-lysine, taurine and IMP induced a similar feeding activity compared to that of the synthetic extract (trial 2 and 3). In trial 4, the highest feeding activity was finally obtained in the mixture of L-histidine, L-glutamine and IMP, followed by the synthetic extract, the mixture of L-lysine, L-alanine and IMP, IMP and the mixture of L-histidine, L-glutamic acid, L-lysine and L-alanine. These results revealed that the mixture of L-histidine, L-glutamic acid and IMP for the proper feeding stimulant of PBT in this study.
양식, 방류 및 자연산 감성돔 1년어의 소화기관 및 체성분 비교
지승철 ( Seung Cheol Ji ),유진형 ( Jin Hyung Yoo ),정관식 ( Gwan Sik Jeong ),명정구 ( Jeong Gu Myeong ),이시우 ( Si Woo Lee ),고현정 ( Hyeon Jeong Go ) 한국수산과학회 2004 한국수산과학회지 Vol.37 No.6
N/A In order to evaluate adaptation to the environment in released fish, digestive organ and body composition were compared among 1-year old cultured (CUL), released (REL) and wild (WIL) Black Sea Bream, Acanthopagrus schlegeli. Hepatosomatic index (HSI) of the REL was significantly lower than those of the CUL and WIL fish, but intestine weight index (IWI) of REL was significantly higher than those of the CUL and WIL fish. There were no significant differences in stomach weight index (SWI) and condition factor (CF) among those different fish groups. The number of pyloric caeca of the REL and WIL fish were 4, but the 30% of CUL fish was 3 counts. The moisture content of whole body in the WIL fish was significantly higher than the CUL and REL fish, but significant lowest in the crude lipid of whole body. The content of moisture and crude protein of dorsal muscle were no significant differences among the CUL, REL and WIL fish, but crude lipid content of WIL fish was significantly lower than the CUL and REL fish. Moisture content of CUL fish in the liver was significantly lower than the REL and WIL fish, and crude lipid was significant lowest in the WIL fish. Amino acid content of dorsal muscle in the WIL fish was highest in the total amino acid, EAA and E/A ratio, and CUL fish was highest EAA and E/A ratio in liver. EPA content in dorsal muscle of WIL fish was lower than CUL and REL fish, and the REL fish was highest in DHA content of the fatty acid. EPA content in liver of WIL fish was lower than CUL and REL fish, and DHA content in REL and WIL fish was highest and lowest. Crude protein content of CUL and WIL fish in the scale was significantly higher than REL fish, but there were no significant differences in contents of crude lipid and ash. Ca and P of scale were formed most of mineral and content of P in CUL fish was significantly higher than those of REL and WIL fish. There were no significant differences in Mg, K, Na and S of scale, but CUL fish was significantly lower than REL fish in Cl. The results suggest that difference of digestive organs and body composition concluded it from differences of inhabitation environment and feed formulations.
넙치용 고효율 습사료 개발의 관한 연구 Ⅱ. 시판분말배합사료의 이용성 평가
정관식(Kwan Sik JEONG),지승철(Seung Cheol JI) 전남대학교 수산과학연구소 1998 수산과학연구소논문집 Vol.7 No.-
The feeding trials were conducted to investigate availability of five different commercial compound meal (CCM) supplemented to a moist pellet (MP) containing raw fish used in practical aquaculture places; qualities of different CCMs used commely in present mariculture were evaluated through these feeding trials. The 9-week feeding trial was conducted to investigate the effects of MPs containing five different CCMs for flounder on growth and body composition of fish. The 19g juvenile flounder grew to 72.1~87.8g after 9-week. Percent weight gain of fish fed various CCM MPs ranged from 281.5% to 357.3%; The MP containing CCM of A Co. produced significantly greater weight gain than the other diet (B, C, D and E Co.). Feed efficiency values were highest (81.1%) in the MP containing CCM of A Co. and followed by the MP (79.4%) containing CCM of B Co. and then, MPs (67.6~72.5%) from the other company. In total feed comsumption, fish fed MPs containing CCM of A Co. had also the greatest consumption; therefore, the CCM of A Co. considered as having a remarkable feed attractability. According to the result of this study, the CCM of A Co. produced greater weight gain, feed efficiency and feed consumption than the other company’s CCMs.
한약재 혼합물 첨가 사료가 넙치와 조피볼락의 성장과체성분에 미치는 영향
김건수(Keon Soo KIM),지승철(Seung Cheol Ji),주용석(Young Seok Joo),정관식(Gwan Sik JEONG) 전남대학교 수산과학연구소 2003 수산과학연구소논문집 Vol.12 No.-
This study was conducted to investigated effects of diet added by herbs mixture (HM, 4 kinds of herbs were mixed) on growth and body composition of olive flounder (Paralichtys olivaceus) and rockfish (Sebastes schlegeli). Juvenile olive flounder with average weighting 15 g was used as experimental fish, and they were accommodated with two replicate group by 50 fishes in the 1×1×1m net cage. Fish fed one of five experimental diets containing 0%, 0.1%, 0.3%, 0.5% and 1.0% HM for 4weeks. Rockfish averaging 93.0 g were fed one of three diets control, containing 0.5% HM, 0.5% HM + GA (garlic acid) 0.5% for 8 weeks. There were no significant difference in survival rate of olive flounder (98~100%), and rockfish was 81.7~90%. Growth rate of olive flounder was the highest in the HM 0.3 group, and 0.5 and 1.0 HM group were significantly higher than the control group, but there were no significant difference between the control and HM 0.1 group. Also, feed efficiency was significantly higher in the HM added groups than the control group and the 0.3% group showed the highest. Growth rate of rockfish was the highest in the HMGA group, and HM group higher than the control group. Also, feed efficiency was similar tendency with growth result. Chemical composition of whole body in olive flounder, there was no significant difference between the control group and the HM added groups. Lipid contents of dorsal muscle was significantly lower in the HM added groups than the control group. Lipid contents of viscera in rockfish was significantly lower in the control group than the HM and HMGA group. These result indicate that the herbs mixture and garlic meal added diet showed high effect on the improvement in growth of olive flounder and rockfish, and 0.3%~0.5% of HM and 0.5% garlic meal addition was judged as proper.