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배재형 ( Jae Hyung Bae ),조상래 ( Seong Eun Kim ),정은희 ( Sang Rae Jo ),장은희 ( Eun Hui Jeong ),김성은 ( Eun Hui Jang ),권희녕 ( Hui Nyeong Kwon ),박동엽 ( Dong Yeop Park ),이국천 ( Kuk Cheon Lee ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.2
Bovine brucellosis, an important zoonosis, is diagnosed with serological tests such as the RBT, TAT using inactivated whole bacterial cells or bacterial lipopolysaccharide (LPS) antigen in Korea. However, a strong cross-reaction between Brucella spp. and Yersinia enterocolitica O9 in these tests has seriously complicated the diagnosis of animal brucellosis because Brucella spp. shares common antigenic determinants with Y. enterocolitica O9 in the smooth LPS region. In this study, Brucella-field strains were isolated from Brucellapositive Hanwoo in Kimhae, Korea and outer membrane protein (omp) which has low cross-reaction with Y. enterocolitica O9 and high immunogenicity was extracted from the field strains Then we compared ELISA using the extract with RBT-TAT. Fifteen field strains were isolated from 47 supramammary-lymph nodes, which were collected from 18 farms. Isolation rate was 32%. Brucella-specific antigen was identified by performing SDS-PAGE or Western blotting on extracted omp with at 0.5% n-lauroylsarcosine One hundred and ninety-two serum-samples were used in the experiment: 142 negative and 50 positive samples verified by RBT-TAT. According to ELISA results, 127 samples were negative and 15 appeared positive among 142 negatives by RBT-TAT, while 42 samples were positive and 8 were negative among 50 positives by RBT-TAT. Therefore, it showed 89.4% of specificity and 84% of sensi-tivity. Through the current experiments, we could set up an ELISA based on the omp which has low cross-reaction and high immunogenicity and concluded that the omp could be a good material for accurate diagnosis of bovine brucellosis.
Aflatoxin에 노출된 닭에서 활성탄과 어성초의 독성완화 효과
하대식 ( Dae Sik Hah ),지대해 ( Dae Hae Ji ),조상래 ( Sang Rae Jo ),박애라 ( Ae Ra Park ),정은희 ( Eun Hee Jung ),박동엽 ( Dong Yeop Park ),이국천 ( Kuk Cheon Lee ),허정호 ( Jung Ho Heo ),김종수 ( Jong Shu Kim ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.2
This study was conducted to evaluate the alleviative effects of activated charcoal (AC) and Houttuynia cordata (HC) singly or in combination in broiler chickens during aflatoxicosis. Activated charcoal (1% or 0.5%) and H. cordata (1% or 0.5%) were mixed into the diets for the ability to reduced the deleterious effects of 2.4mg total aflatoxin (AFB1) kg-1 diet on growing broiler chickens from 1 to 21 days of age. A total of 160 1-day-old (Hyline Variety Brown) broiler chicks were housed in eight treatment groups [Control, AFB1, AC 1%, HC 1%, AFB1 plus AC 1% plus HC 1%, AFB1 plus AC 1% plus HC 0.5%, AFB1 plus AC 0.5% plus HC 1%, AFB1 plus AC 0.5% plus HC 0.5%] each consisting of 20 chicks. Compared to control, 2.4mg AFB1 alone treatment group significantly decreased body weight gains of chickens. The addition of mixed AC 1% and HC 1% including 6, 7 groups to the 2.4mg AFB1-containing diet moderately reduced the adverse effects of AFB1 on performances of chickens. The chickens consuming 2.4mg AFB1 plus AC 0.5% and HC 0.5%-containing diet showed very slightly reduced the adverse effects on investigated parameters compared to the AFB1 only treated group. Also, the single addition of AC or HC to the AFB1-free diet had no adverse effects in chickens. These results suggest that AC and HC mixed can reduced the aflatoxicosis in broilers and may be contribute to a solution of the aflatoxicosis problem in poultry production.
도축장의 소와 돼지 분변에서 분리한 살모넬라속의 약제내성 및 약제내성 유전자의 보유율
하도윤 ( Do Yun Hah ),지대해 ( Dae Hae Ji ),조상래 ( Sang Rae Jo ),박애라 ( Ae Ra Park ),정은희 ( Eun Hee Jung ),박동엽 ( Dong Yeop Park ),이국천 ( Kuk Cheon Lee ),양정웅 ( Jung Wung Yang ),김종수 ( Jong Shu Kim ),김혜정 ( Hye Jun 한국가축위생학회 2011 韓國家畜衛生學會誌 Vol.34 No.1
This study was conducted to investigate the distribution of Salmonella spp. from pigs and cattle in slaughterhouse, the antimicrobial resistance pattern and the prevalence of resistance genes of isolates. A total of 640 fecal samples from pigs and cattle in slaughterhouse were collected for isolation of Salmonella spp.. Isolation rate was revealed as 15% in pigs and 1.6% in cattle. As result of serotyping, group B (56.6%) were identified as most common in pigs and cattle isolates, in order of group C (24.5%) and group E (15.1%). S. Typhimurium (50.9%) was most common serotype. The major serotypes were in order of S. Rissen and S. London (11.3%) and S. Riggil (7.6%). In antimicrobial test, all isolates were demonstrates susceptibility to nitrofurantoin. But isolates were revealed resistance other antibiotics in order of tetracycline (64.6%), streptomycin (68.3%), ampicillin and amoxicillin (56.3%) and spectinomycin (47.9%). With polymerase chain reaction, antimicrobial resistance gene strA (75.0%) and aadA1 (3.1%) were detected in streptomycin resistance isolates and tetA (94.3%) and tetB (11.3%) gene were detected in tetracycline resistant isolates, but tetG was not detected. Class 1 integron gene was detected in all Salmonella isolates.
경남지역에서 도축우 및 돼지의 근육내 잔류항균물질 검색
박동엽 ( Dong Yeob Park ),양평섭 ( Pyeong Seob Yang ),남창우 ( Chang Woo Nam ),황보원 ( Bo Won Hwang ),김원규 ( Won Gyu Kim ),조상래 ( Sang Rae Jo ),김도경 ( Toh Kyung Kim ) 한국가축위생학회 2002 韓國家畜衛生學會誌 Vol.25 No.3
The present studies were carried out to determine antibiotics residues in pork and beef muscles by EEC-4-plate and HPLC. A total of 2,534 samples of pork muscles and 1,070 samples of beef muscles from slaughter houses were collected in Gyeongnam area from January to December, 2001. The results were summarized as follows; 1. Recovery rates of TCs, Sulfa drug, Penicillin G from fortified pork and beef muscles ranged as 68.79~98.24%, 78.21~94.58% and penicillin G 63.35~67.24% respectively, by HPLC. 2. Antibiotics residues were detected in 36 sample(1.42%) of pork muscles, 29 sample (2.71%) of beef muscles by EEC-4-plate. 3. Detection rate of antibiotic residues 14 samples(0.55%) and 26 samples(2.43%), in pork and beef muscles, respectively by HPLC. Concentration of residues in 22 sample(2.06%) of beef muscle were higher than tolerance level in korea. 4. Antibiotics detected were sulfamethazine(47.37%), tetracycline(15.79%), oxytetracycline(15.79%), penicillin G(15.79%), sulfamerazine(5.26%) in pork muscle samples and oxyteracycline(37.21%), penicillin G(30.23%), sulfamethazine(20.93%), tetracycline(4.65%), sulfamerazine(2.33%), sulfadimethoxine(2.33%), sulfaquinoxine(2.33%) in beef muscle samples.