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      • SCOPUSKCI등재

        솔잎혹파리의 염색체주기 및 침샘염색체 연구

        임낙룡 한국유전학회 1985 Genes & Genomics Vol.7 No.2

        The cytological studies dealing with the karyotypes of the somatic cells and chromosome behavior in gametogenetic cell cycles in both male and female of Thecodiplosis japonensis, Cecidomyiidae (Diptera) were made. The karyotypes in somatic nuclei were consisted of 6 chromosomes in the male and 8 in the female. On the other hand, the haploid male was composed of 4 chromosomes which originated from the 40 chromosomes in the germ-line, while the female gamete contained 20 chromosomes, 4 S-chromosomes and 16 E-chromosomes. Therefore, the fertilization among such sperm and egg restores the full complement of 24 chromosomes. Upon sexual differentiation into either male or female from the zygotes, it is assumed that sex determination depends on E-gene(s) locating on one of the two kinds of X-chromosome pairs (White, 1950, 1977). The salivary gland nuclei have four polytene chromosomes. Of these, one carries only one nucleolar organizer. However there was no chromocenter for four polytene chromosomes. From an anterior part of the salivary gland cells of larvae parasitized by Hymenoptera, polytene polyploids were found.

      • SCOPUSKCI등재

        초파리 citrate synthase cDNA 의 클로닝과 염기서열 결정

        임낙룡,박은규 한국유전학회 1991 Genes & Genomics Vol.13 No.4

        To analyse the relationship between time and the allelism of lethal genes (second and third chromosomes together as a unit), wild males of Drosophila melanogaster were captured at different times in a given point of a Korean locality and separated into two experimental groups with month-interval and year-interval in time. Each of the two groups contained five of time series populations. The average frequencies of flies carrying lethal genes were 0.8407 in the month-interval populations and 0.8202 in the year-interval populations. The rates of allelism of lethals were 0.0475 in the month-intervals and 0.0184 in the year-intervals. In both cases, it showed that the slope of the allelism of lethals decreased exponentially with time interval far apart. The average of effective sizes in the present D. melanogaster population were estimated to approximately be 1,000 in the month-intervals and 1.500 in the year-intervals.

      • 노랑초파리에서 전이인자 hobo의 행동

        임낙룡,남궁용 全北大學校 科學敎育硏究所 1999 과학과 과학교육 논문지 Vol.24 No.-

        A few crosses, inbreeding and hybrid crosses, with Uc-1 strain carried out to learn about the mechamism of hobo activity. A transposable element hobo for the Notch locus of the Uc-1 X chromosome, which does not interfere with the normal expression of the locus, interacts with other hobo elements in the same X chromosome to procuce Notch mutations. The phenotype of Notch wing mutation has been produced by deletion of 3C region in X chromosome of D. melanogaster. The Uc-1 X chromosome produces the Notch mutation at rate of 1.09%∼5.09% in inbred crosses for 29 generations. The mutation rate showed a subtle trend to fluctuate at ±2.69%. It seems that hobo transposable elements have activity in Uc-1 inbred strain. On the other hand, inter-crosses between Uc-1 strain and non Uc-1 strains had done to find whether the cytotype of maternal lines and genome of paternal lines effect on the hobo activity. The result in F_1 hybrid between crosses of Uc-1 strain and E(empty)strains showed obvious reciprocal cross effect. E strains do not have any hobo element. Notch mutaion rates was appeared over 5% in F_1 hybrid from crosses which Uc-1 males mated with E strain female(Cross A), but was under 1% in hybrid F_1 of their reciprocal crosses(Cross B). This result means that there was maternal effect in the mechanism of hobo activity. Notch mutation rates which was found in F_1 hybrid with CH(Complete hobo) strain was similar to the rates obtained from crosses with E strains. CH strain contains only complete hobo elements. There was no any clue that complete hobo elements contained genome effect on the hobo activity. The comparable result was obtained from the analysis of crosses that Uc-1 strain mated with CDH(Complete and defective hobo)strain which contains complete and delete hobo element. Notch mutants hardly appeared in Cross A with CDH but Cross B produced Notch mutant rates at the level of the rates in crosses which was done with other strains. The result suggest that delete hobo elements possibly act a role of hobo represser.

      • 전주 초파리 집단내 유전성 변이에 관한 연구

        임낙룡,권국일 全北大學校 敎育大學院 1997 敎育論叢 Vol.17 No.-

        The genetic studies on lethal gene frequencies and inversion polymorphisms from Chonju natural populations of Drosophila melanogaster were carried out. To analyse lethal gene frequencies and allelism of lethal genes in intra- and inter-population, two populations collected in November of 1986 and 1987 were tested. The frequency of genome carrying deleterious gene was 80.28%. The average frequency of allelic lethals was 0.0148 in within population and 0.0118 in between population. The rate of elimination of lethal genes by homozygosis(IQ^2) was 0.0024. And the effective population size of the present population sampled was estimated to be about 3,000 pairs of flies. To test inversion polymorphism, 832 males sampled in October and November of 1986 and 1987 were examined. Every wild caught male was crossed to three virgin females with standard chromosomal sequencies in all chromosome. A total of eight different inversions were found and identified to be autosomal paracentric types only. Seven of them were cosmopolitan inversions and the rest endemic one. The average frequency of flies carryoing inversions was 0.352 and mean number of inversions per individual fly was 0.454. The frequency order observed was 2Lt > 3RP > 3LP > 3RC > 2RNS > 2LK > 3RMo(P=0.0013 with Friedman's rank test). Although the linkage disequlibrium was detected between left and right arm of third chromosome, the present Chonju populations was in good fitted to the Hardy-Weinberg equilibrium.

      • SCOPUSKCI등재

        한국 초파리 집단에서의 유전성 변이

        임낙룡,권국일,박승태 한국유전학회 1998 Genes & Genomics Vol.20 No.2

        The genetic studies on lethal gene frequencies and inversion polymorphisms from Chonju natural populations of Drosophila melanogaster were carried out. To analyse lethal gene frequencies and allelism of lethal genes in intra- and inter-population, a total of 410 males collected in Novembers of 1986 and 1987 were tested. The frequency of both second and third chromosomes, as a unit, carrying deleterious gene was 80.28%. The average frequency of allelic lethals was 0.0148 in within population and 0.0118 in between population. The rate of elimination of lethal genes by homozygosis (IQ²) was 0.0024. And the effective population size of the present population sampled was estimated to be about 3,000 pairs of flies. To test inversion polymorphism, a total of 832 males sampled in Octobers and Novembers of 1986 and 1987 were examined. Each wild caught male was crossed to three virgin females with standard chromosomal sequencies in all chromosome. A total of eight different inversions were found and identified to be autosomal paracentric types only. Seven of them were cosmopolitan inversions and the rest endemic one. The average frequency of flies carrying inversions was 0.352 and mean number of inversions per individual fly was 0.454. The frequency order observed was 2Lt > 3RP > 3LP > 3RC > 2RNS > 2LK > 3RMo (P=0.0013 with Friedman's rank test). Although a linkage disequilibrium was detected between left and right arm of third chromosome, the present Chonju populations was in good fitness to the Hardy-Weinberg equilibrium.

      • SCOPUSKCI등재

        초파리 지역집단간에서 치사인자의 동향

        임낙룡,권국일 한국유전학회 1995 Genes & Genomics Vol.17 No.4

        To analyse the relationship between distance and the allelism of lethal genes, wild males of Drosophila melanogaster were captured in four Korean natural populations, Suwon, Taejon, Chonju and Naju. Using a balanced stock, known as A₁B_(18) which is translocated with second and third chromosomes and can balance two major autosomes simultaneously, two major autosomes were extracted as a single unit. The average frequencies of flies carrying lethal genes were 0.8067. The allelic rate of lethals were 1.35% within population, and 0.56% between populations. The rate of elimination of lethals by homozygosis (1Q²) was an average of 0.0022 in four populations. Effective sizes of the population were ranged from 2,068 to 5,865. The regression analysis showed that the slope of the allelism of lethals decreased with distance far apart.

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