우유중에 함유되어있는 잔류 항생물질의 검사법

이희무 ( Hee Moo Lee ) 한국균학회 1983 韓國菌學會誌 Vol.11 No.3

새로운 항균활성을 보이는 토양 분리 세균 Paenibacillus polymyxa DY1의 분류와 동정

신은석,이희무,이복권,김성훈,권순일,유관희,Shin, Eun-Seok,Lee, Hee-Moo,Lee, Bok-Kwon,Kim, Sung-Hoon,Kwon, Sun-Il,Yoo, Kwan-Hee 한국미생물학회 2007 미생물학회지 Vol.43 No.1

항생제 내성 세균의 출현으로 새로운 항생물질의 개발에 대한 필요성이 대두되고 있다. 본 연구에서는 새로운 항균활성물질을 개발하고자 강원도 대암산 용늪 토양으로부터 새로운 항균물질을 생산하는 균을 분리하였고, 이를 동정하였다. 생화학적인 시험과 16S ribosomal DNA 염기서열 분석결과 Paenibacillus polymyxa균과 가장 높은 상동성을 보여주었다. 지방산 조성의 분석에서도 이 균주는 Paenibacillus polymyxa와 가장 가까웠다. 이 균주가 생산하는 항균물질은 1군 법정 전염병을 일으키는 Samonella enterica serovar Typhi와 Shigella dysentery, enterohaemorrhagic Eschelichia coli, 그리고 Vibrio cholera등의 병원성 세균에 성장억제 효과를 나타냈으며, 다른 일반 식중독 장내세균에서도 성장억제 효과를 나타냈다. 이 균주가 생산하는 항균활성 물질은 과거에 보고된 것과 다른 새로운 것으로 보이며, 광범위한 항균활성으로 인하여 새로운 항생물질 개발 후보로 많은 잠재력을 가진 것으로 평가된다. The DY1 strain of Gram-positive, rod-shaped bacteria was isolated from the soil sample collected from Daeam mountain, Korea. The culture filtrate of DY1 strain showed a broad spectrum of antimicrobial activity on various pathogenic and food poisoning enteric bacterial species tested in vitro. It showed significant growth-inhibitory effect on Salmonella enterica sp., Shigella sp., pathogenic Escherichia coli, Vibrio cholerae, Vibrio parahemolyticus, and Yersinia enterocolitica. For the identification of the DY1 strain, morphological, biochemical and molecular phylogenetic approaches were performed. The DY1 strain was found to be a member of the genus Paenibacillus on the basis of morphological and biochemical analyses. The 16S rDNA of DY1 showed the highest pairwise identity with Paenibacillus polymyxa with 99.79% (1,413 bp/1,416 bp). The antimicrobial entity from DY1 looked different from preciously reported ones and seems to have a great potential to be further studied as a candidate of new antibiotics to control multi-drug resistant pathogens.

최근 한국에서 유행하는 장염비브리오균의 분자 역학적 특성

김상숙(Sang-Sook Kim),이희무(Hee-moo Lee),이중복(Jung-Bok Lee) 한국생물공학회 2003 KSBB Journal Vol.18 No.6

경북지역 가축에서 분리된 Salmonella typhimurium과 S enteritidis의 병원성 시험

김상윤 ( Sang Yun Kim ),이희무 ( Hee Moo Lee ),김신 ( Sin Kim ),홍현표 ( Hyon Pyo Hong ),권헌일 ( Heon Il Kwon ) 한국가축위생학회 2001 韓國家畜衛生學會誌 Vol.24 No.1

The result of studying the pathogenicity of Salmonella typhimuriwn and S enteritidis isolated from domestic animals in Gyeongbuk province were summarized as follows. In Congo-red binding test, S typhimurium had much more rough types than S enteritidis. In colicin production test, 4 strains of S typhimurium were positive but all of S enteritidis were negative. In hemolysin production test, all of S typhimurium and S enteritidis were negative. In Guinea pig serum resistant test, all of S typhimurium and S enteritidis were positive. As a result of pathogenicity test to mice, 54.4% of mice were died. Therefore, S typhimurium and S enteritidis were considered as highly pathogenic. S typhimurium DT104 and S enteritidis PT4 were more pathogenic to mice than other phage types of same serovar. S typhimurium and S enteritidis were considered not so pathogenic for 6-day-old chickens. The recovery rates of Salmonella strains from mice and chickens inoculated were 96.8%, and 54%, respectively. In chickens, proportional to the time From 2 weeks after challenge inoculation. The recovery rates were noticeably decreased.

경북지역 가축에서 Salmonella속 균 감염증에 대한 역학적 특성

김상윤 ( Sang Yun Kim ),이희무 ( Hee Moo Lee ),김신 ( Sin Kim ),홍현표 ( Hyon Pyo Hong ),권헌일 ( Heon Il Kwon ) 한국가축위생학회 2001 韓國家畜衛生學會誌 Vol.24 No.1

The result of studying the epidemiological characteristics of Salmonella strains which have been isolated from the domestic animals in Gyeongbuk province from February 1998 to August 2000 were summarized as follows. The isolation rates of Salmonella strains were 2.0% from cattle feces, 6.3% from cattle lymph node, 9.5% from pig feces, and 25.1% from pig lymph node. In poultry, the isolation rates were 30.3%. The isolates of Salmonella showed positive reaction for MUCAP test, methyl red test, but showed negative reaction for urea test, indole test, Voges Proskauer test. On TSI agar, the isolates showed acid butt, alkaline slant. Also, the isolates were identified as Salmonella strain by API 20E kit. Non H2S production Salmonella strains isolated from poultry were identified as S gallinarum. As a result of serotyping, B group were the most common in cattle and pig, D1 in chickens. 21 serovars were found. The common serovar from the domestic animals was S typhimurium, S derby, S agona, S schwarzenground, S enteritidis and S gallinarum. The most commonly encountered serovars in cattle were S agona and S typhimurium in pig, S gallinarum in chicken. As a result of antimicrobial susceptibility test, all Salmonella isolates were susceptible to amikacin, ciprofloxacin. Norfloxacin, cefotaxime and polymycin B. The resistance rates to tetracycline and streptomycin was 58% and 56% respectively. 69.3% of all isolates were resistant to more than one antimicrobial agent. Out of the resistant isolates, the isolates resistant to streptomycin and tetracycline was 36%. There were 24 strains of multiresistant isolates resistant to more than 5 antimicrobial agents. S typhimurium were resistant to all antimicrobial agents, also had a lot of multiresistant strains. Therefore, S typhimurium was considered as a major agent of antimicrobial resistance.

경북지역 가축에서 분리된 Salmonella typhimurium과 S enteritidis의 phage typing 및 pulsed-field gel electrophoresis

김상윤 ( Sang Yun Kim ),이희무 ( Hee Moo Lee ),김신 ( Sin Kim ),홍현표 ( Hyon Pyo Hong ),권헌일 ( Heon Il Kwon ) 한국가축위생학회 2001 韓國家畜衛生學會誌 Vol.24 No.3

Forty-five Salmonella typhimurium isolates were encountered 8 phage types in which DT197 and U302 were the predominant types. The DT104 type which was first found from pig in Korea, and was resistant to chloramphenicol, streptomycin, sulfamethoxazole/trimethoprim, tetracycline, gentamicin and nalidixic acid. Twenty-two S enteritidis isolates were encountered 5 phage types in which PT4 were the representative (predominant). S enteritidis isolates were susceptible to all antimicrobial agents. As a result of PFGE analysis for S typhimurium and 5 enteritidis, PFGE patterns was better than phage typing in discriminating of strains. PFGE patterns were not in accord with phage type even though some strain had the same phage types.

Bacillus thuringiensis serovar kurstaki 돌연변이균주의 편모항원의 변이성

이형환(Hyung - Han Lee),이영주(Young - Chu Lee),이희무(Hee - Moo Lee) 한국미생물생명공학회 1989 한국미생물·생명공학회지 Vol.17 No.1

간 및 위 , 장관을 침범한 특발성 과호산구 증후군 치험

정을순(Eul Soon Chung),이상종(Sang Jong Lee),김향(Hyang Kim),김병익(Byung Ik Kim),황기은(Kie Eun Hwang),성기철(Ki Chul Sung),조용균(Yong Kyun Cho),이희무(Hee Moo Lee),금주섭(Joo Seob Keum) 대한소화기학회 1997 대한소화기학회지 Vol.30 No.3

Marked eosinophilia is found in many kinds of disease state, It usually occur in allergic and parasitic conditions and occ assionally in autoimmune disease, blood dyscrasias, or certain malignancics. Idio- pathic hypereosinophilic syndrome is characterized by markedly elevated peripheral blood eosinophil count without explainable etiology. Eosinophilopoiesis is related with T-lymphocyte activation and cytokine cascade controlling eosinophilic production. It shows tissue infiltration in many organs including endomyocardium, lung, liver, gastrointestinal tract. We have recently experienced a case with idiopathic hypereosinophilic syndrome of a 65-year-old woman who had of complained anorexia, weight loss and diarrhea during the past month and half. Persistantly increased eosinophil count was shown in her peripheral blood, the count averaging 3480/mm'(41% in differential count). Abdominal computer tomography disclosed multiple small relatively ill-defined hypoechoic lesions in the liver. Microscopically, the needle biopsy of the liver revealed heavy infiltration by eosinophilis especially in the portal tracts. On reviewing, previously perfomed endoscopic biopsies from the stomach and terminal ileum also had mild to moderate eosinophilic infiltration, respectively. No evidence of parasitic infection was present on the clinical history and laboratory test. Corticosteroid was administered ard brought marked improvement of clinical manifestations including both hematologic and radiologic abnormalities. (Korean J Gastroenterol 1997; 30:397- 403)

유방암 유즙에서 분리한 포도구균의 분자생물학적 typing과 multiplex PCR을 이용한 장독소의 검출

김신 ( Shin Kim ),홍현표 ( Hyon Pyo Hong ),김상윤 ( Sang Yun Kim ),권헌일 ( Heon Il Kwon ),이희무 ( Hee Moo Lee ) 한국가축위생학회 2002 韓國家畜衛生學會誌 Vol.25 No.3

Forty strains of Staphylococcus aureus were isolated from mastitic milk. As a result of antimicrobial susceptibility test, the strains of S aureus revealed 47.5% were resistant to ampicillin and penicillin, and 7.5% to gentamicin. But 45% of isolates were sensitive to antimicrobial agents tested. In case of enterotoxin production, 56.3% of 16 strains produced enterotoxin D. Two strain of enterotoxin D producers produced both enterotoxin B and D. According to isolation date, 15 representative strains were selected. As a results of pulsed field gel eletrophoresis analysis of the 15 representative strains, 14 strains were identical. Therefore we consider the identical strains of S aureus have caused continuously bovine mastitis in this dairy farm. If autogenous vaccine can be made by the strains, it will work well for the prevention of bovine mastitis caused by S aureus.

토양에서 분리된 아연내성균의 아연흡착에 관한 연구

이희무,이장순,이중복 안동대학교 환경연구소 2001 환경연구 Vol.1 No.-

본 연구는 중금속으로 오염된 폐수의 생물학적 처리 기술의 개발을 목적으로 수행하였다. 그래서 이 연구에서는 토양에서 Zn 내성균을 분리하여 분리된 균의 Zn 흡착실험을 실시하였다. 흡착실험에서는 흡착제의 sorption kinetics와 흡착등온선 그리고 pH, 온도와 같은 다양한 요인에 의한 Zn의 제거능을 조사하였다. 연구 결과는 다음과 같다. 1. 토양에서 Zn 내성균을 분리하여 그 균의 형태적인 특징을 조사하였다. 그 결과 균은 Penicillium속인 것으로 판명되어, Penicillium sp. HA113으로 명명하였다. 그리고 균의 최적 배양조건은 온도 25℃∼30℃, pH 5.5∼7.0인 것으로 조사되었다. 2. Penicillium sp. HA113을 흡착제로 사용하기 위해 다음과 같은 처리를 하였다. Penicillium sp. HA113의 mycelium을 배양하여 증류수로 세척한 다음, 80℃, 24hr 동안 건조시켜 grinding하였다. Grind한 mycelium을 흡착제로 이용하였고, 흡착제의 particle size는 100.00㎛이하이다. 3. Sorption kinetics 실험에서 흡착제의 Zn 흡착율은 처음에서 증가하다가 1시간이내에 흡착평형에 도달하였다. 4. Sorption isotherm 실험은 서로 다른 초기 Zn농도로 실시되었고 얻어진 실험 data를 Freundlich 방정식에 적응시켰다. 그 결과, 흡착제의 Zn 흡착은 효과적인 것으로 나타났다. 5. pH와 온도 변화에 따른 흡착제의 Zn 제거율은 다음과 같은 결론을 내렸다. 흡착제의 pH 영향에 Zn 흡착 실험에서 낮은 pH에서는 흡착율이 낮게 나타났고, 높은 pH에서는 흡착율이 높게 나타났다. 그리고 온도변화에 의한 흡착제의 Zn 흡착실험에서는 흡착제의 Zn 흡착율과 온도의 영향은 큰 관계가 없었다. This study was performed to develop the biological treatment technology of wastewater polluted with heavy metals. This study was isolated the heavy-tolerant microorganism in soil and was conducted the Zinc adsorption experiments. In the adsorption study, the influence of sorption kinetics and sorption isotherm and various parameters such as pH, temperature was investigated. The results of the study are as follows. 1. It was investigated the morphological character of the isolated Zinc-tolerant microorganism and was named Penicillium sp. HA113. The optimum culturing condition Penicillium sp. HA113 was temperature 25℃∼30℃, pH 5.5∼7.0. 2. After culturing the mycelium of Penicillium sp. HA113, it was washing the deionized water. Then, mycelium was dried in 80℃, 24hr and was grinding. This was used as adsorbent. The particle size of adsorbent was less than 100.00㎛. 3. In sorption kinetics experiments, adsorption rate was initially rapid and the extent of adsorption arrived at adsorption equilibrium in an hour. 4. The sorption isotherm experiment was made with different initial Zinc concentration. A linearized Freundlich equation was to fit the acquired experimental data. As a result, it was concluded that sorption of Zinc by adsorbent is effective. 5. The effect of pH on Zinc sorption by adsorbent shows that the removal efficiency of Zinc of adsorbent was increased in high pH than low pH. And the effect of temperature on heavy sorption by adsorbent shows that temperature wasn't related with the removal efficiency of Zinc of adsorbent.