건선병변의 T 림프구 아형 분포에 관한 연구

성경제,김현수,서정화,최지호 ( Kyung Jeh Sung,Hyun Soo Kim,Jung Wha Suh,Jee Ho Choi ) 대한피부과학회 1997 大韓皮膚科學會誌 Vol.35 No.3

Background: Although the main features of psoriasis consist of abnormal epidermal proliferation and T cell infiltration, which of these is the initial abnormality has been a longstanding unresolved question. Recently there has been definite evidence that activated T cells produce the cytokines that induce keratinocyte abnormalities. Objective : We investigated the distributions and relative numbers of T lymphocyte subpopulations, that is, CD4+ and CD8+ T cells, to verify the more important T cell subtype and its infiltrating site in the formation of psoriatic lesions. Methods : Paired psoriatic lesional and non-lesional skin tissues were obtained from eight typical psoriatic patients. Immunohistochemical staining was done on the frozen sections using a labelled streptavidin-biotin peroxidase complex method with DAKO LSAB kit. The primary antibodies used in this study were monoclonal or polyclonal antibodies against CD3, CD4, CD8, HLA-DR, and ICAM-1. Resulis : In lesional psoriatic skin, the epidermis was mainly infiltrated by CD8+ T cells. There were little or no T cells in non-lesional epidermis. In both lesional and non-lesional dermis, CD4+ T cells were the main ones. In lesional skin, anti-ICAM-1 antibody positively stained diffusely in the endothelial cells of papillary and subpapillary plexuses and focally in epidermal keratinocytes, but in non-lesional skin only the endothelial cells in the subpapillary plexus were stained. There were many HLA-DR+T lymphocytes in lesional and non-lesional dermis. In the epidermis, HLA DR was detected only in lesional keratinocytes and T lymphocytes. Conclusion : These results suggest (1) participation of activated epidermal CD8+ T lymphocytes in the formation of psoriatic plaques, and (2) a pathogenetic role of ICAM-1 expression on papillary microvessels. (Kor J Dermatol 1997;35(3): 435-442)

S - 100α 및 β 단백질에 대한 단세포군 항체를 이용한 한선종양의 면역조직화학연구

성경제(Kyung Jeh Sung),조광현(Kwang Hyun Cho),서호석(Ho Seok Suh),최지호(Jee Ho Choi),고재경(Jai Kyoung Koh) 대한피부과학회 1992 대한피부과학회지 Vol.30 No.4

S-100 protein is a mixture of three proteins, that is, S-100 ao(aa), S-100 a(ab) and, S- 100 b(bb). Twenty-two case, of sweat gland tumors were stained with immunoperoxidase technique (ABC method) for the presence of S-100a and b-subunit. Four syringomas, four eccrine poromas, two eccrine porocarcinomas, two ecerine spirdeiomas, one papillary eccrine adenoma, three clear cell hidradenomas, three mixed tumr rs of the skin, two papillary syringocystadenomas, and one cylindroma were included. All specimens were formalin-fixed and paraffin-embedded. The results were as follows : 1) The staining patterns of anti-S-100a and b-protein antibodies we e simillar to those of anti-S-100 protein antibody except in eccrine poroma and porocare nomal. 2) In eccrine poroma and porocarcinoma, scattered S-100-positive dendritic cells within tumor cell nests were stained by S-100-protein antibody (3/6), but not by anti-S-100a protein antibody. S-100p is present in normal Langerhans cells. Therefore this finding suggests that these cells niay be Langerhans cells. (Kor J Dermatol 1992;30(4):446-453)

한국형 여드름 중증도 시스템

성경제 ( Kyung Jeh Sung ),노영석 ( Young Suck Rho ),최응호 ( Eung Ho Choi ),오정준 ( Jeong Joon Oh ),이주흥 ( Joo Heung Lee ),김선우 ( Seon Woo Kim ),김낙인 ( Nack In Kim ) 대한피부과학회 2004 대한피부과학회지 Vol.42 No.10

한국인에서 경도 및 중등도의 여드름에 대한 경구 Isotretinoin의 유효성 및 안전성 평가

성경제(Kyung Jeh Sung),최응호(Eung Ho Choi),황상민(Sang Min Hwang),서대헌(Dae Hun Suh),이승헌(Seung Hun Lee) 대한피부과학회 2000 대한피부과학회지 Vol.38 No.10

Background:Oral isotretinoin has been widely used in severe acne patients and is known as a very effective drug. Recently it is being used in any grades and any types of acne. However we have few reports of clinical study about oral isotretinoin in our country. Objective:To investigate clinical efficacy, factors influencing the result and side effects of oral isotretinoin in mild to moderate acne patients in Korea, prospectively. Materials and methods:The patients with mild to moderate acne visiting out-patient dermatology clinics of four university hospitals have taken oral isotretinoin 0.3-1.0mg/kg/day over 16 weeks. The clinical improvement was assessed by the scores using photographs before treatment and at 8 weeks, 12 weeks, 16 weeks, 20 weeks after treatment, and by the satisfaction of patients and physicians at the end of treatment. The side effects were recorded and SGOT, SGPT, cholesterol and triglyceride were checked at regular intervals. Results:The grade of acne was much improved proportional to the duration of treatment. The factors showing better results were dosage and duration of oral isotretinoin, older age, more severe acne and severe acne in the past. About 90% of patients and physicians were satisfied with the result at the end of treatment. The side effects, mainly mucocutaneous symptoms were expectable and tolerable in the most cases. The values of SGOT, SGPT, cholesterol and triglyceride were increased after treatment but within normal reference range. Conclusion:Oral isotretinoin is a very effective agent in Korean patients with mild to moderate acne. We expect the better result in patients having high dose and long duration of oral isotretinoin, old ages and more severe acne. If we understand it's side effects enough, we can use it very safely. (Korean J Dermatol 2000;38(10):1309~1314)

한선종양에 대한 면역조직화학적 연구

성경제(Kyung Jeh Sung),조광현(Kwang Hyun Cho),정홍근(Hong Keun Chung),김성범(Sung Bum Kim),최지호(Jee Ho Choi),고재경(Jai Kyoung Koh) 대한피부과학회 1992 대한피부과학회지 Vol.30 No.3

The histogenesis and differentiation of sweat gland tumors are controversial. Twenty-two cases of sweat gland tumors were stained by immunoperoxidase technique (ABC method) for the presence of S-100 protein, CEA, and two kinds of keratin. Four syringomas, 4 eccrine poromas, 2 eccrine porocarcinomas, 2 eccrine spiradenomas, 1 papillary eccrine adenoma, 3 clear cell hidradenomas, 3 mixed tumors of skin, 2 papillary syringocystadenomas, and 1 cylindroma were included. All samples were formalin-fixed and paraffin-erribedded. Two monoclonal cytokeratin ant.ibodies, MA-902 (specific for cytokeratin No. 8) and MA-903 (specific for cytokeratins No.1,5,10,11) were used. In normal eccrine and apocrine glands, MA-902 stains cells of the intradermal duct and secretory portion. While MA-903 stains cells of the intraepidermal and intradermal duct and myoepithelial cells of eccine and apocrine glands, S-100 protein is found in the secretory cells of the intradermalduct and secretory portion, while CEA stains the secretory and ductal cells of eccrine and apocrine glands. All sweat gland tumors we studied stained by 4 antibodies in variable positive rates, Based on these findings, we discuss the histogenesis of various sweat gland tumors. (Kor J Dermatol 1992; 30(3): 303-316)

5 - FU로 인한 홍반성감각둔감 증후군

성경제(Kyung Jeh Sung),정환교(Hwang Gyo Jeong),고재경(Jai Kyoung Koh) 대한피부과학회 1990 대한피부과학회지 Vol.28 No.4

Erythr odysesthesia syndrome is a. peculiar toxic reaction due to anti-cancer drug. lt is characterized by a painful, purple-colored patch on the palms and soles, and may form bullae. We report a case of erythrodysesthesia syndrome in a 46-year-old woman. 5-FU and leucovorin were administered for 5 days because of her colon cancer. Two weeks after anti-cancer therapy, purple-colored erosive patch appeared on the face, and 10 days later, purple-colored vesicular patches were seen on both palms and soles, accompanied with pain. Histopathologic examination reveals a severe epidermal necrosis. Individual keratinocyte shows cytologic atypia and apoptotic body. (Kor J Oermatol 28(4): 450 455, 1990)

구리 증기 레이저와 Flashlamp Pumped Pulsed 색소 레이저 치료에 따른 부작용의 비교에 관한 연구

성경제 ( Kyung Jeh Sung ),이상협 ( Sang Hyup Lee ),이화정 ( Hwa Jung Lee ),고재경 ( Jai Kyoung Koh ),최지호 ( Jee Ho Choi ) 대한피부과학회 1995 대한피부과학회지 Vol.33 No.5

Background : There are two kinds of mechanism for laser therapy according to selectie photothermolysis. Selective photothermolysis means that a chromophore can be selectively dei, stroyed with a laser of n appropriate wavelength and of a short pulse duration that is shortor than the thermal relaxation time of the chromophore. It is supposed that the side effects of the continuous wave laser, in which selective photothermolysis does not exist occur more often than when a pulsed wave laser is used. Objective : We compar.d the side effects of flashlamp-pumped pulsed tunable dye laser(SPTL), whose treatment is baseal on selective photothermolysis, with those of copper vapor laser(CVL , which is a quasi-continuous thermal laser. Methods : The laser cl arts of 498 patients with SPTL treatment and 485 patients with CVL, treatment in Asan Medical Center from 1989 to 1994 were examined, retrospectively. Results : 1) Among 498 patients, with SPTL treatment, we observed various side effects in 47(9.4%). The incidences of individial side effects are as follows, hyperpigmentation in 6.2%(31), vesicl formation in 1.6% (8), surface texture change in 0.8% (4), Hypopigmentation in 0.6% (3), scar format,ion in 0.2% (1). 2) After CVL treatment, side effects occurred in 30(6.2%) among 485 patient.. Hyperpigmentation in 3.1%(15), vesicle formation in 2.5%(12), surface texture change in 0.2%(1), hypopigmentation in 0.2%(1), and scar formation in 0.1%(1) were observed. Conclusion : The total incidence of side effect.s after CVL treatment is not statistically different, from that after SPTL treatment, which contradicts the previous theory. (Kor J Dermatol 1995;33(5): 815-820)

연화제와 UVB를 이용한 병용치료로 호전된 요독성 소양증 6예

성경제(Kyung Jeh Sung),김성범(Sung Bum Kim),최지호(Jee Ho Choi),고재경(Jai Kyoung Koh) 대한피부과학회 1992 대한피부과학회지 Vol.30 No.4

Phototherapy with UVB has become a cornerstone in treating uremic pruritus. To investigate the additional benefit of emollient, we performed emollien-phototherapy in six uremic pruritus patients on hemodialysis, one of which was not responding with UVB only. After applying mineral oil over the whole body, UVB phototh.rapy was performed two to three times weekly. Improvement was noted within one to three treatments and after three to ten treatments, pruritus markedly or totally disappeared in al six patients. This result suggests that emollient-phototherapy is as effective as, or in some patients, more effective than phototherapy with UVB only in managing uemic pruritus. (Kor J Dermatol 1992;30(4):546-550)

혈액투석중인 만성 신부전증 환자의 피부증상 및 이에 대한 혈액투석의 영향

성경제(Kyung Jeh Sung),이미우(Mi Woo Lee),최지호(Jee Ho Choi),고재경(Jai Kyoung Koh),박수길(Soo Kil Park) 대한피부과학회 1991 대한피부과학회지 Vol.29 No.3

The purpose of this study was to examine the frequency of eutaneous disorders in patients with chronie renal failure presently on hemodialysis(HD). The results were as follows. 1. Cutaneous lesions were present in all 78(100% ) patients with chronic renal failure. 2. The frequency of msjor cutaneous problems associated with chronic renal failure was as follows: xerosis(82.1%), pruritus(74.4%), nail changes(74.4%), hyperpigmentation(70.5 % ), xerostomia(42.3'Yo), poor wound healing(37.2%), easy bruisability(30,8%), hypotrichosis(21.896), and purpura(14.1 % ). 3. Cutaneous problems which appeared after the initiation of HD were. appearance of new pigmented nevi or lentigines(9 patients), appearance or aggravation of acne(6), contact dermatitis at AV fistula site(2), gynecomastia(1), extensive flat warts(1), extensive tinea versicolor(1), and extensive vitiligo(1). 4. Among 55 patients with hyperpigmentation, 11 patients reported decreased pigmentation following HD. In 15 patients, hyperpigmentation worsened following HD, and in 9 patients hyperpigmentation first appeared after HD was initiated. 5. Cutaneous disorders favorably affected by HD were as follows: easy bruisability(3/ 24), xerostomia(3/33), gingival friability(2/7), and hypotrichosis(5/17). 6. Poor wound healing and xerosis were not improved by HD. 7. Among 58 pruritic patients, 7 patients were improved after HD, 7 patients became worse during each HD, and 3 patients were not pruritic only for 2 to 3 days after each HD. Four patients experinced pruritus only during each HD. 8. There was no significant statistical difference between the frequency of pruritus and xerosis and the levels of blood urea nitrogen, calcium, phosphorus, and parathyroid hormone. (Kor J Dermetol 20(3): 313 321,1901)

건선 표피에서의 p53, pRb, p16, p21 및 PCNA에 대한 면역조직화학적 고찰

장성은(Sung Eun Chang),성경제(Kyung Jeh Sung),문기찬(Kee Chan Moon),고재경(Jai Kyoung Koh),최지호(Jeo Ho Choi) 대한피부과학회 1999 대한피부과학회지 Vol.37 No.9

N/A Background : Psoriasis is a chronic disorder which is characterized by hyperproliferation and abnormal differentiation of keratinocytes with T-cell infiltration. There is increasing evidence that inactivation of tumor-suppressor genes can promote uncontrolled cell proliferation and tumor formation. The products of p53, Rb, p16, p21 genes are examples of the molecules the cell growth cycle. Objective : In this study, immunhistochemical analysis of cell cycle-related molecules (p53, pRb, p16, p21Wafl, and PCNA) was done to elucidate the roles and interactions of those molecules in the pathogenesis of psoriatic keratinocytes. Materials and methods : Formalin-fixed skin samples composed of 20 chronic plaque type psoriatic, 20 nonlesional and 20 normal epidermis, and 5 squamous cell carcinomas were included in this study. Results : Our results suggest that p53 protein accumulation is an early event in cutaneous SCC, but not in psoriasis. In our study, diffuse expression of p16 with almost negative expression of pRb in psoriatic epidermis suggest that inverse relationship is maintained. Although loss of p16 expression is frequently associated with many kinds of cancers, we speculate there is a possibility that is a strong expression of p16 is related to localized cancer as well as in benign hyperproliferative lesions. In psoriasis, p21 may be overexpressed via a p53-independent pathway. The number of PCNA positive cells was higher in the psoriatic epidermis in contrast to scattered staining in the psoriatic nonlesional and normal epidermis Conclusion : The proliferative status of psoriatic leratinocytes dose not implicate Rb gene and may not implicate the p53 gene significantly. It is associated with the strong expression of PCNA and moderately increased expressions of p16 and p21. (Korean J Dermatol 1999;37(9) : 1286∼1295)